Light and carbon sources addition alter microbial community in biofloc-based Litopenaeus vannamei culture systems

Aquaculture ◽  
2020 ◽  
Vol 515 ◽  
pp. 734572 ◽  
Author(s):  
Wenwen Jiang ◽  
Wenjing Ren ◽  
Li Li ◽  
Shuanglin Dong ◽  
Xiangli Tian
Keyword(s):  
Microbial Community ◽  
Litopenaeus Vannamei ◽  
Carbon Sources ◽  
Culture Systems

scholarly journals The Effects of Different Carbon Sources on the Production Environment and Breeding Parameters of Litopenaeus vannamei

Water ◽  
2021 ◽  
Vol 13 (24) ◽  
pp. 3584
Author(s):  
Yiming Xue ◽  
Li Li ◽  
Shuanglin Dong ◽  
Qinfeng Gao ◽  
Xiangli Tian
Keyword(s):  
Water Quality ◽  
Growth Rate ◽  
Microbial Community ◽  
Litopenaeus Vannamei ◽  
Carbon Sources ◽  
Ammonia Removal ◽  
Total Alkalinity ◽  
Production Environment ◽  
Microbial Composition ◽  
Linear Discriminant

This study investigated the effect of different carbon sources on water quality, ammonia removal pathways, the bacterial community, and the production of Litopenaeus vannamei in outdoor culture tanks. Three systems were established: a clear water system (CW) and biofloc technology (BFT) systems with added molasses (M-BF) or poly (3-hydroxybutyric acid-co-3-hydrovaleric acid) (PHBV) (P-BF). The average pH, total alkalinity, total organic carbon, biofloc volume, chlorophyll a, nitrite, nitrate, total nitrogen, and nitrification rate were significantly different among the treatments. Microbial composition varied and different dominant taxa were identified in the treatments by linear discriminant analysis effect size. Redundancy analysis indicated that the water quality parameters affected the distribution of the microbial community. Moreover, the genus Leucothrix was closely related to the M-BF treatment. Chemoheterotrophy and aerobic chemoheterotrophy were the most abundant functions in all treatments. A comparison of functions using BugBase indicated that the relative abundance of several functions such as biofilm formation, stress tolerance and functions related to anaerobic processes increased in the M-BF treatment. The specific growth rate, growth rate, and survival rate of shrimp were significantly higher in the P-BF system than in the CW system and the feed conversion ratio in the BFT treatments was significantly lower than that in the CW system. Overall, adding carbon sources affected water quality, microbial community, and shrimp performance. The results show that PHBV is a good alternative to carbon sources.

Assessment of different ionic adjustment strategies in low‐salinity water on the growth of Litopenaeus vannamei and microbial community stoichiometry in a synbiotic nursery system

2021 ◽  
Author(s):  
Otávio Augusto Lacerda Ferreira Pimentel ◽  
Valdemir Queiroz Oliveira ◽  
Caio Rubens do Rêgo Oliveira ◽  
William Severi ◽  
Alfredo Olivera Gálvez ◽  
...  
Keyword(s):  
Microbial Community ◽  
Litopenaeus Vannamei ◽  
Low Salinity ◽  
Low Salinity Water ◽  
Salinity Water ◽  
Adjustment Strategies

scholarly journals A metaproteomics method to determine carbon sources and assimilation pathways of species in microbial communities

2018 ◽  
Author(s):  
Manuel Kleiner ◽  
Xiaoli Dong ◽  
Tjorven Hinzke ◽  
Juliane Wippler ◽  
Erin Thorson ◽  
...  
Keyword(s):  
Microbial Community ◽  
Microbial Communities ◽  
Carbon Isotope ◽  
Isotope Ratio ◽  
Single Cells ◽  
Stable Carbon Isotope ◽  
Carbon Sources ◽  
Food Sources ◽  
Individual Species ◽  
Stable Carbon

AbstractMeasurements of the carbon stable isotope ratio (δ13C) are widely used in biology to address major questions regarding food sources and metabolic pathways used by organisms. Measurement of these so called stable carbon isotope fingerprints (SIFs) for microbes involved in biogeochemical cycling and microbiota of plants and animals have led to major discoveries in environmental microbiology. Currently, obtaining SIFs for microbial communities is challenging as the available methods either only provide limited taxonomic resolution, such as with the use of lipid biomarkers, or are limited in throughput, such as NanoSIMS imaging of single cells.Here we present “direct Protein-SIF” and the Calis-p software package (https://sourceforge.net/projects/calis-p/), which enable high-throughput measurements of accurate δ13C values for individual species within a microbial community. We benchmark the method using 20 pure culture microorganisms and show that the method reproducibly provides SIF values consistent with gold standard bulk measurements performed with an isotope ratio mass spectrometer. Using mock community samples, we show that SIF values can also be obtained for individual species within a microbial community. Finally, a case study of an obligate bacteria-animal symbiosis showed that direct Protein-SIF confirms previous physiological hypotheses and can provide unexpected new insights into the symbionts’ metabolism. This confirms the usefulness of this new approach to accurately determine δ13C values for different species in microbial community samples.SignificanceTo understand the roles that microorganisms play in diverse environments such as the open ocean and the human intestinal tract, we need an understanding of their metabolism and physiology. A variety of methods such as metagenomics and metaproteomics exist to assess the metabolism of environmental microorganisms based on gene content and gene expression. These methods often only provide indirect evidence for which substrates are used by a microorganism in a community. The direct Protein-SIF method that we developed allows linking microbial species in communities to the environmental carbon sources they consume by determining their stable carbon isotope signature. Direct Protein-SIF also allows assessing which carbon fixation pathway is used by autotrophic microorganisms that directly assimilate CO2.

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