scholarly journals Diagnosis of Streptococcus canis periprosthetic joint infection: the utility of next-generation sequencing

2018 ◽  
Vol 4 (1) ◽  
pp. 20-23 ◽  
Author(s):  
Majd Tarabichi ◽  
Abtin Alvand ◽  
Noam Shohat ◽  
Karan Goswami ◽  
Javad Parvizi
Keyword(s):  
Next Generation Sequencing ◽  
Periprosthetic Joint Infection ◽  
Joint Infection ◽  
Next Generation ◽  
Streptococcus Canis ◽  
Generation Sequencing

Next-generation sequencing not superior to culture in periprosthetic joint infection diagnosis

2021 ◽  
Vol 103-B (1) ◽  
pp. 26-31
Author(s):  
Beau J. Kildow ◽  
Sean P. Ryan ◽  
Richard Danilkowicz ◽  
Alexander L. Lazarides ◽  
Colin Penrose ◽  
...  
Keyword(s):  
Next Generation Sequencing ◽  
Periprosthetic Joint Infection ◽  
Joint Infection ◽  
Next Generation ◽  
Sample Collection ◽  
Tissue Samples ◽  
Musculoskeletal Infection ◽  
Conventional Culture ◽  
Significant Difference ◽  
Generation Sequencing

Aims Use of molecular sequencing methods in periprosthetic joint infection (PJI) diagnosis and organism identification have gained popularity. Next-generation sequencing (NGS) is a potentially powerful tool that is now commercially available. The purpose of this study was to compare the diagnostic accuracy of NGS, polymerase chain reaction (PCR), conventional culture, the Musculoskeletal Infection Society (MSIS) criteria, and the recently proposed criteria by Parvizi et al in the diagnosis of PJI. Methods In this retrospective study, aspirates or tissue samples were collected in 30 revision and 86 primary arthroplasties for routine diagnostic investigation for PJI and sent to the laboratory for NGS and PCR. Concordance along with statistical differences between diagnostic studies were calculated. Results Using the MSIS criteria to diagnose PJI as the reference standard, the sensitivity and specificity of NGS were 60.9% and 89.9%, respectively, while culture resulted in sensitivity of 76.9% and specificity of 95.3%. PCR had a low sensitivity of 18.4%. There was no significant difference based on sample collection method (tissue swab or synovial fluid) (p = 0.760). There were 11 samples that were culture-positive and NGS-negative, of which eight met MSIS criteria for diagnosing infection. Conclusion In our series, NGS did not provide superior sensitivity or specificity results compared to culture. PCR has little utility as a standalone test for PJI diagnosis with a sensitivity of only 18.4%. Currently, several laboratory tests for PJI diagnosis should be obtained along with the overall clinical picture to help guide decision-making for PJI treatment. Cite this article: Bone Joint J 2021;103-B(1):26–31.

scholarly journals Metagenomic next-generation sequencing of synovial fluid demonstrates high accuracy in prosthetic joint infection diagnostics

2020 ◽  
Vol 9 (7) ◽  
pp. 440-449 ◽  
Author(s):  
Zida Huang ◽  
Wenbo Li ◽  
Gwo-Chin Lee ◽  
Xinyu Fang ◽  
Li Xing ◽  
...  
Keyword(s):  
Next Generation Sequencing ◽  
Synovial Fluid ◽  
Prosthetic Joint Infection ◽  
Joint Infection ◽  
High Accuracy ◽  
Next Generation ◽  
Primary Arthroplasty ◽  
Prosthetic Joint ◽  
Culture Positive ◽  
Generation Sequencing

Aims The aim of this study was to evaluate the performance of metagenomic next-generation sequencing (mNGS) in detecting pathogens from synovial fluid of prosthetic joint infection (PJI) patients. Methods A group of 75 patients who underwent revision knee or hip arthroplasties were enrolled prospectively. Ten patients with primary arthroplasties were included as negative controls. Synovial fluid was collected for mNGS analysis. Optimal thresholds were determined to distinguish pathogens from background microbes. Synovial fluid, tissue, and sonicate fluid were obtained for culture. Results A total of 49 PJI and 21 noninfection patients were finally included. Of the 39 culture-positive PJI cases, mNGS results were positive in 37 patients (94.9%), and were consistent with culture results at the genus level in 32 patients (86.5%) and at the species level in 27 patients (73.0%). Metagenomic next-generation sequencing additionally identified 15 pathogens from five culture-positive and all ten culture-negative PJI cases, and even one pathogen from one noninfection patient, while yielding no positive findings in any primary arthroplasty. However, seven pathogens identified by culture were missed by mNGS. The sensitivity of mNGS for diagnosing PJI was 95.9%, which was significantly higher than that of comprehensive culture (79.6%; p = 0.014). The specificity is similar between mNGS and comprehensive culture (95.2% and 95.2%, respectively; p = 1.0). Conclusion Metagenomic next-generation sequencing can effectively identify pathogens from synovial fluid of PJI patients, and demonstrates high accuracy in diagnosing PJI. Cite this article: Bone Joint Res 2020;9(7):440–449.

scholarly journals Metagenomic next-generation sequencing contribution in identifying prosthetic joint infection due to Parvimonas micra: a case report

2019 ◽  
Vol 4 (1) ◽  
pp. 50-55 ◽  
Author(s):  
Zida Huang ◽  
Chongjing Zhang ◽  
Wenbo Li ◽  
Xinyu Fang ◽  
Qijin Wang ◽  
...  
Keyword(s):  
Case Report ◽  
Next Generation Sequencing ◽  
Prosthetic Joint Infection ◽  
Joint Infection ◽  
Next Generation ◽  
Prosthetic Joint ◽  
Parvimonas Micra ◽  
Culture Independent ◽  
Generation Sequencing

Abstract. Identifying fastidious pathogens in patients with prosthetic joint infection (PJI) by culture is challenging. Metagenomic next-generation sequencing (mNGS) is a novel culture-independent approach that is associated with a higher likelihood for identifying pathogens. We present a case where mNGS was implemented to identify Parvimonas micra, a rarely reported and difficult-to-culture PJI pathogen.

scholarly journals Diagnostic value of next-generation sequencing to detect periprosthetic joint infection

2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Han Yin ◽  
Duliang Xu ◽  
Dawei Wang
Keyword(s):  
Next Generation Sequencing ◽  
Blood Cell ◽  
Periprosthetic Joint Infection ◽  
Bacterial Culture ◽  
Joint Infection ◽  
Diagnostic Value ◽  
Infection Group ◽  
Serological Biomarkers ◽  
Generation Sequencing ◽  
D Dimer

Abstract Background We herein compared the diagnostic value of next-generation sequencing (NGS), bacterial culture, and serological biomarkers to detect periprosthetic joint infection (PJI) after joint replacement. Methods According to the diagnostic criteria of the Musculoskeletal Infection Society, 35 patients who underwent joint revision surgery were divided into infection (15 cases) and non-infection (20 cases) groups, and were routinely examined preoperatively for erythrocyte sedimentation rate (ESR), C-reactive protein (CRP), procalcitonin (PCT), interleukin-6 (IL-6), and D-dimer levels. All patients underwent arthrocentesis preoperatively. Synovial fluid was used for white blood cell count, white blood cell classification, bacterial culture, and NGS. Furthermore, we calculated the area under the curve (AUC) of the receiver operating characteristic curve (ROC) for ESR, CRP, PCT, IL-6, and D-dimer. Data were assessed by comparing diagnostic accuracy, sensitivity, and specificity. Results Fourteen patients showed positive results by NGS and seven showed positive bacterial culture results in the infection group; further, 18 showed negative results by NGS in the non-infection group. The AUC of ESR, D-dimer, CRP, IL-6, and PCT was 0.667, 0.572, 0.827, 0.767, and 0.808, respectively. The accuracy of NGS, bacterial culture, CRP, IL-6, and PCT was 0.91, 0.74, 0.77, 0.74, and 0.83, respectively. When comparing NGS with CRP, IL-6, PCT, and bacterial culture, differences in overall test results and those in sensitivity were statistically significant, and compared with CRP, differences in specificity were also statistically significant. In comparison with IL-6, PCT, and bacterial culture, the specificity of NGS was statistically insignificant. Conclusions Our results indicated that NGS had higher accuracy and sensitivity than the bacterial culture method and commonly used serological biomarkers for diagnosing PJI.

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