Effect of age dependent cold storage of factitious host Corcyra cephalonica (Stainton) (Lepidoptera: Pyralidae) for their continuous production and Trichogramma chilonis (Ishii) (Hymenoptera: Trichogrammatidae) rearing

2017 ◽  
Vol 20 (3) ◽  
pp. 928-934 ◽  
Author(s):  
Enakshi Ghosh ◽  
Chandish R. Ballal
2007 ◽  
Vol 7 (1) ◽  
pp. 30-38
Author(s):  
F.X. Susilo ◽  
Desta S. Romli ◽  
Sunaryo Sunaryo ◽  
M. Solikhin

Early Detection of Trichogramma chilonis sexes using the egg color and size of its factitious host Corcyra cephalonica. This study was aimed to detect the sexes of Trichogramma chilonis Ishii (Hymenoptera: Trichogrammatidae) prior to their emergence using the egg color and size of their factitious host, Corcyra cephalonica (Lepidoptera: Pyralidae). A gray scale of 1 – 7 was used to rank the egg color while a micrometer was used to observe the egg size under a dissecting microscope. Ten of newly laid C. cephalonica eggs were glued in two rows (0.5 cm x 0.4 cm distance) on a piece of oviposition paper. Five replications of such batches were exposed in a test tube to a gravid T. chilonis female for 24 hours under 100 watts of illumination.  Following the withdrawal from the oviposition arena the eggs were each observed daily for their color and size and the sex of the emerged T. chilonis progeny was documented.  Binomial Z-test at 0.05 level of significance was used to validate the predicted proportion of T. chilonis sexes from five batches of 50 parasitized C. cephalonica eggs. The results showed as predicted that more T. chilonis female progeny emerged from light-colored C. cephalonica eggs (scale 2 – 5) following oviposition by parent parasitoid or from larger C. cephalonica eggs (> 500 µm).


2019 ◽  
Vol 4 (01) ◽  
pp. 49-54
Author(s):  
Ravi Kumar ◽  
Ajay Kumar ◽  
Rajendra Singh ◽  
Joginder Singh ◽  
Ankit Kumar ◽  
...  

The present investigations were undertaken in laboratory on “Study on factitious host Corcyra cephalonica (Stainton) against storage of egg parasitoid Trichogramma chilonis (Ishii) cards under laboratory conditions” during the year 2018 in the bio-control laboratory, Department of Agricultural Entomology, College of Agriculture, Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut (U. P.) India. The effects of different rearing media on some biological parameters of factitious host, C. cephalonica are based on results are most effective performance with T3 (Sorghum + Groundnut + Yeast powder, 50:50 + 5g Yeast), treatments i.e. larval, pupal, male and female moths emergence, and egg production in cc and followed by 17530, 16667, 15131,14875, 13545 and 11830 i.e. T3 (Sorghum + Groundnut + Yeast powder, 50:50 + 5g Yeast), T5 (Sorghum + Soyabeen + Yeast powder, 50:50 + 5g Yeast), T6 (Sorghum + Cowpea + Yeast powder, 50:50 + 5g Yeast), T4 (Sorghum + Gram powder + Yeast powder, 50:50 + 5g Yeast), T2 (Sorghum + Green Gram + Yeast powder, 50:50 +5g Yeast), T1 (Sorghum + Black gram + Yeast powder, 50:50 + 5g Yeast) and T7 (Sorghum alone (check) eggs per cc of Sorghum + Groundnut + Yeast powder, 50:50 + 5g Yeast and wheal), respectively.


2017 ◽  
Vol 107 (6) ◽  
pp. 791-798 ◽  
Author(s):  
A.P. Queiroz ◽  
A. de Freitas Bueno ◽  
A. Pomari-Fernandes ◽  
M.L.M. Grande ◽  
O.C. Bortolotto ◽  
...  

AbstractEgg parasitoid rearing on factitious hosts is an important step to reduce costs and increase availability of the biocontrol agent but it requires quality control to achieve success in field conditions. To this end, this study evaluated the quality of Telenomus remus (Hymenoptera: Platygastridae) reared on Corcyra cephalonica (Lepidoptera: Pyralidae) for until 45 generations. In the first bioassay, we evaluated the body size of the laboratory-produced parasitoids. In the second bioassay, flight activity was examined, measuring the percentage of ‘flyers’, ‘walkers’ and ‘deformed’ parasitoids. The third bioassay assessed parasitism on Spodoptera frugiperda (Lepidoptera: Noctuidae) eggs. Our data indicate that the laboratory-reared parasitoid neither lost its ability to fly nor to parasitize S. frugiperda eggs. In conclusion, quality did not decrease significantly during 45 generations, and therefore rearing of T. remus on C. cephalonica as factitious host promises to be successful.


2018 ◽  
Vol 124 ◽  
pp. 40-45 ◽  
Author(s):  
Han Wu ◽  
Yan-Chang Huang ◽  
Ji-Xing Guo ◽  
Jian-Bo Liu ◽  
Xue-Shuang Lai ◽  
...  

2018 ◽  
Vol 17 (2) ◽  
pp. 128-138
Author(s):  
Atirach NOOSIDUM ◽  
Weerachai SOMSRI ◽  
Angsumarn CHANDRAPATYA

Habrobracon hebetor has been investigated as a successful biological control agent for larvae of pyralid moths in warehouses. Gaining new information on how to improve the storage procedure of H. hebetor under cold conditions could allow the development of approaches to maintain this parasitoid in pest management programs. This study investigated the effect of cold temperatures (10 and 15 °C) on the development of H. hebetor pupae reared on Corcyra cephalonica, which was kept for up to 3 weeks. Mortality of H. hebetor pupae during cold storage in all treatments was 4 - 19 %. Mortality of H. hebetor adults emerging during cold storage at 10 °C (28 %) and 15 °C (61 %) was significantly observed at 21 days of storage, while other treatments were less than 4 %. Numbers of emerging adults after cold storage from 7 - 14 days were 89 - 96 %, and the female percentage ranged from 13 - 39 %. The number of eggs laid per female from the emerging females after cold storage for 7 - 14 days was not significant as compared to the control treatment (43 - 53 eggs per female) after laying for 72 h. The sex ratios of emerging adults in all cold storages and the control treatment at 72 h after emerging were not significant.


2013 ◽  
Vol 104 (2) ◽  
pp. 203-212 ◽  
Author(s):  
A. Valigurová ◽  
V. Michalková ◽  
P. Koník ◽  
M.L. Dindo ◽  
M. Gelnar ◽  
...  

AbstractThe tachinid fly Exorista larvarum (L.) (Diptera: Tachinidae) is a polyphagous larval endoparasitoid that deposits its eggs on the host exoskeleton of lepidopteran and tenthredinid larvae. The attachment of larval E. larvarum and the formation of the respiratory funnel were studied during infestation in the last larval instar of the wax moth, Galleria mellonella (L.) (Lepidoptera: Pyralidae). The tachinid larvae burrow through the host integument after hatching, using their robust cephalopharyngeal skeleton, leaving a dark spot at the point of their penetration as a result of host cuticle melanization. Endoparasitoid penetration induces the host cellular defence, resulting in the formation of a haemocyte capsule consisting of multi-cellular sheaths. This enveloping capsule later undergoes melanization, which is mostly obvious towards the posterior part of the endoparasitoid. The endoparasitoid uses the host encapsulation response to build a respiratory funnel from the modified host integument, leading to the host surface. The encapsulated larva remains attached to the respiratory funnel via an anal hook and cuticular spines until fully developed. Additional immunohistochemical analyses were used to study host–parasitoid interactions. Indirect immunofluorescence showed no labelling of potential tachinid antigens and confirmed no effect on the surrounding host tissues. A simulated parasitization with coated polybead microspheres revealed the mortal impact of tachinid antigens to the host. Hosts injected with antigen-coated polybeads died as a consequence of an acute and extensive immunological response to the tachinid antigens and not due to the trauma caused by foreign objects inside their body.


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