Quality control of Telenomus remus (Hymenoptera: Platygastridae) reared on the factitious host Corcyra cephalonica (Lepidoptera: Pyralidae) for successive generations

2017 ◽  
Vol 107 (6) ◽  
pp. 791-798 ◽  
Author(s):  
A.P. Queiroz ◽  
A. de Freitas Bueno ◽  
A. Pomari-Fernandes ◽  
M.L.M. Grande ◽  
O.C. Bortolotto ◽  
...  

AbstractEgg parasitoid rearing on factitious hosts is an important step to reduce costs and increase availability of the biocontrol agent but it requires quality control to achieve success in field conditions. To this end, this study evaluated the quality of Telenomus remus (Hymenoptera: Platygastridae) reared on Corcyra cephalonica (Lepidoptera: Pyralidae) for until 45 generations. In the first bioassay, we evaluated the body size of the laboratory-produced parasitoids. In the second bioassay, flight activity was examined, measuring the percentage of ‘flyers’, ‘walkers’ and ‘deformed’ parasitoids. The third bioassay assessed parasitism on Spodoptera frugiperda (Lepidoptera: Noctuidae) eggs. Our data indicate that the laboratory-reared parasitoid neither lost its ability to fly nor to parasitize S. frugiperda eggs. In conclusion, quality did not decrease significantly during 45 generations, and therefore rearing of T. remus on C. cephalonica as factitious host promises to be successful.

2019 ◽  
Vol 4 (01) ◽  
pp. 49-54
Author(s):  
Ravi Kumar ◽  
Ajay Kumar ◽  
Rajendra Singh ◽  
Joginder Singh ◽  
Ankit Kumar ◽  
...  

The present investigations were undertaken in laboratory on “Study on factitious host Corcyra cephalonica (Stainton) against storage of egg parasitoid Trichogramma chilonis (Ishii) cards under laboratory conditions” during the year 2018 in the bio-control laboratory, Department of Agricultural Entomology, College of Agriculture, Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut (U. P.) India. The effects of different rearing media on some biological parameters of factitious host, C. cephalonica are based on results are most effective performance with T3 (Sorghum + Groundnut + Yeast powder, 50:50 + 5g Yeast), treatments i.e. larval, pupal, male and female moths emergence, and egg production in cc and followed by 17530, 16667, 15131,14875, 13545 and 11830 i.e. T3 (Sorghum + Groundnut + Yeast powder, 50:50 + 5g Yeast), T5 (Sorghum + Soyabeen + Yeast powder, 50:50 + 5g Yeast), T6 (Sorghum + Cowpea + Yeast powder, 50:50 + 5g Yeast), T4 (Sorghum + Gram powder + Yeast powder, 50:50 + 5g Yeast), T2 (Sorghum + Green Gram + Yeast powder, 50:50 +5g Yeast), T1 (Sorghum + Black gram + Yeast powder, 50:50 + 5g Yeast) and T7 (Sorghum alone (check) eggs per cc of Sorghum + Groundnut + Yeast powder, 50:50 + 5g Yeast and wheal), respectively.


2021 ◽  
Vol 21 (2) ◽  
pp. 158-165
Author(s):  
Adha Sari ◽  
Damayanti Buchori ◽  
Ihsan Nurkomar

Effect of host-larval diet on the host acceptance and host suitability of the egg parasitoid Telenomus remus Nixon (Hymenoptera: Scelionidae) on Spodoptera frugiperda J. E. Smith (Lepidoptera: Noctuidae). The life history of parasitoids is an important factor that can determine their ability to attack a host. The type of food consumed by the host can affect the ability of parasitoids such as host searching behavior, host suitability and host acceptance. In this research, we evaluate the effect of the S. frugiperda larvae diet on its suitability of the eggs produced by the adults for the egg parasitoid Telenomus remus. The research was studied on two types of egg masses of S. frugiperda that obtained from the moths that fed with natural or artificial diet during their larval stages. Parasitoid was reared from both types of hosts. An egg mass consisting of 50 S. frugiperda eggs from both types of hosts was exposed to one egg parasitoid female for 24 hours. S. frugiperda eggs then were reared until the parasitoid adult emerged. Each experiment was repeated 20 times. Host acceptance was observed through the host parasitism rate and its parasitization. Meanwhile, the host suitability was observed through the sex ratio of the emerging parasitoids. The results showed that S. frugiperda eggs reared using artificial diet had a higher parasitism rate (99.33%) than those of natural diet (82.53%). In contrast, the level of parasitization of S. frugiperda eggs reared using natural diet was higher (78.30%) than those of artificial diet (48.34%) because the number of emerging T. remus from S. frugiperda eggs reared using natural diet was also higher than those of artificial diet. However, the sex ratio (F:M) of emerging T. remus from S. frugiperda eggs reared using both of diet was female biased.


2014 ◽  
Vol 104 (6) ◽  
pp. 724-730 ◽  
Author(s):  
O.C. Bortolotto ◽  
G.V. Silva ◽  
A. de Freitas Bueno ◽  
A.F. Pomari ◽  
S. Martinelli ◽  
...  

AbstractGenetically modified crops with insect resistance genes fromBacillus thuringiensisBerliner (Bt-plants) are increasingly being cultivated worldwide. Therefore, it is critical to improve our knowledge of their direct or indirect impact not only on target pests but also on non-target arthropods. Hence, this study evaluates comparative leaf consumption and performance ofSpodoptera eridania(Cramer), a species that is tolerant of the Cry1Ac protein, fed withBtsoybean, MON 87701×MON 89788 or its non-Btisoline. We also assessed the comparative performance of the egg parasitoidTelenomus remusNixon on eggs ofS. eridaniaproduced from individuals that fed on these two soybean isolines as larvae. Results showed thatBtsoybean reduced by 2 days larval development and increased by 3 days adult male longevity. Therefore, we conclude that the effect ofBtsoybean MON 87701×MON 89788 onS. eridaniadevelopment and reproduction is small, and favorable to pest development. These differences are less likely to directly result from the toxin presence but indirectly from unintended changes in plant characteristics caused by the insertion of the transgene. Our results should be viewed as an alert thatS. eridaniapopulations may increase inBtsoybeans, but on the other hand, no adverse effects of this technology were observed for the egg parasitoidT. remuswhich can help to preventS. eridaniaoutbreaks on these crops.


2020 ◽  
Vol 88 (1) ◽  
pp. 116-130
Author(s):  
О. Ye. Lubenchenko ◽  
А. V. Lysiuk

The requirements to the system of control over the quality of audit services are analyzed, which are specified in the Law of Ukraine “Audit of Financial Statements and Auditing” from 21.12.2017 No 2258-VIII; Directive of the Ministry of Finance of Ukraine “Approval of the Procedure for Inspection of the Quality Control of Audit Services” from 29.08.2019 No 362; Resolution of the Council for Supervision over Auditing at the State Institution “The Body of Public Supervision over Auditing” from 30.08.2019 No 6/7/13 “Information Questionnaire of Auditing Entity”. It is demonstrated that the quality control system requires corrections of the following: responsibilities of administration and staff of auditing entities for use of the adopted intra-firm provisions on the quality control system, requirements of ISQC1, IAS, the Law of Ukraine “Audit of Financial Statements and Auditing”; the requirements to staff (quality controller, reviewer, assignment partner); identification of threats to the independence and the auditor’s response on the assessed threats; the requirements to reviews of complaints, complicated issues; issues related with non-stop professional training of auditors. Special attention of auditors need to be drawn to the fact that the policies and procedures of the quality control system do not have to be confined to the audit of financial statements, but have to apply to other assignments on giving confidence and related auditor services. Besides that, assessment of threats to the independence has to be conducted for non-auditor services specified in Article 6 of the aforementioned Law of Ukraine. The studies conducted by the author allowed to elaborate procedures for quality control of auditor services; measures to stimulate staff of auditing entities to scrutinize requirements to implementation of the quality control system; obligating documents of auditing firms on assessment of ethical threats; sharing of duties among assignment group members; requirements to qualification of the key partner, quality controller; identification of auditor assignments; solutions of complicated issues and their documenting,  measures on control of the assignment at the final phase and quality control of the assignment. The proposed measures are supposed to help auditing entities revise the already operating quality control systems in a timely manner, which will facilitate supply of high quality information to all the users of auditing reports, external quality controllers from the Body of Public Supervision over Auditing and the Auditor Chamber of Ukraine.


2007 ◽  
Vol 7 (1) ◽  
pp. 30-38
Author(s):  
F.X. Susilo ◽  
Desta S. Romli ◽  
Sunaryo Sunaryo ◽  
M. Solikhin

Early Detection of Trichogramma chilonis sexes using the egg color and size of its factitious host Corcyra cephalonica. This study was aimed to detect the sexes of Trichogramma chilonis Ishii (Hymenoptera: Trichogrammatidae) prior to their emergence using the egg color and size of their factitious host, Corcyra cephalonica (Lepidoptera: Pyralidae). A gray scale of 1 – 7 was used to rank the egg color while a micrometer was used to observe the egg size under a dissecting microscope. Ten of newly laid C. cephalonica eggs were glued in two rows (0.5 cm x 0.4 cm distance) on a piece of oviposition paper. Five replications of such batches were exposed in a test tube to a gravid T. chilonis female for 24 hours under 100 watts of illumination.  Following the withdrawal from the oviposition arena the eggs were each observed daily for their color and size and the sex of the emerged T. chilonis progeny was documented.  Binomial Z-test at 0.05 level of significance was used to validate the predicted proportion of T. chilonis sexes from five batches of 50 parasitized C. cephalonica eggs. The results showed as predicted that more T. chilonis female progeny emerged from light-colored C. cephalonica eggs (scale 2 – 5) following oviposition by parent parasitoid or from larger C. cephalonica eggs (> 500 µm).


2016 ◽  
Vol 60 (2) ◽  
pp. 177-181 ◽  
Author(s):  
Aline Pomari-Fernandes ◽  
Adeney de Freitas Bueno ◽  
Sérgio Antonio De Bortoli

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