A novel herbal product based on Piper betle and Sphaeranthus indicus essential oils: Toxicity, repellent activity and impact on detoxifying enzymes GST and CYP450 of Aedes aegypti Liston (Diptera: Culicidae)

Light green essential oils extracted from the leaves of Eupatorium odoratum (Asterace) from Himalaya region (India) were screened for repellent activity against Anopheles stephensi, Aedes aegypti and Culex quinquifaciatus mosquitoes. The repellent activity was evaluated in door condition. Four concentrations 2.5%, 5%, 7.5% and 10% (v/v) in ethanol were considered in this study. When the test oil was applied at 10% concentration were found most effective and provided more than 2hr of complete protection against all three mosquito species. The complete protection time (Mean ± S.D) were recorded as 160.2 ± 2.77min, 139.4 ± 1.14min and 125.6 ± 3.13min against Anopheles stephensi, Aedes aegypti and Culex quinquifaciatus respectively. There was no bite within 125min and 160min confirm due to action of essential oil and thus 100% protection from the bite of mosquitoes achieved. No adverse effects of the human volunteers were observed during the exposure and through three months after the application.Therefore, the essential oil of Eupatorium odoratum (Asterace) may prove useful in the development of mosquito natural repellents as an effective personal protection measure against mosquito bites.

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Chemical Composition and Repellent Activity against Mosquito Aedes Aegypti of Pelargonium radula, Syzygiumaromaticum and Citrus aurantifolia Essential Oils

Materials Science Forum ◽

10.4028/www.scientific.net/msf.981.253 ◽

2020 ◽

Vol 981 ◽

pp. 253-257

Author(s):

Hazrulrizawati Abd Hamid ◽

Nishantini Silvarajoo ◽

Nurulhusna Ab. Hamid

Keyword(s):

Chemical Composition ◽

Essential Oil ◽

Aedes Aegypti ◽

Essential Oils ◽

Dengue Fever ◽

Yellow Fever ◽

Repellent Activity ◽

Mosquito Repellent ◽

Citrus Aurantifolia ◽

Frequent Use

The mosquito Aedes aegypti is an epidemic vector of several diseases such as dengue fever and yellow fever. Several pesticides are used to control the mosquito population. Because of their frequent use, some mosquitoes have developed resistance. In the present study, we evaluated the synergistic mosquito-repellent activity of essential oils from Pelargonium radula, Syzgium aromaticum and Citrus aurantifolia against Aedes aegypti by using Y-tube olfactometer. The oils was subsequently analyzed by using GC–MS. These results clearly reveal that the essential oil of C. aurantifolia served as the most potent repellent agent against Aedes aegypti . The results indicate that three constituents; limonene (19.58%) followed by β–pinene (17.12%), geraniol (13.23%) which comprise a large proportion of the C. aurantifolia are likely responsible for the observed repellent activity.

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Repellent activity of three essential oils against dengue and filarial vector mosquitoes

IOP Conference Series Earth and Environmental Science ◽

10.1088/1755-1315/918/1/012001 ◽

2021 ◽

Vol 918 (1) ◽

pp. 012001

Author(s):

D Meisyara ◽

D Tarmadi ◽

A Zulfitri ◽

A Fajar ◽

S K Himmi ◽

...

Keyword(s):

Aedes Aegypti ◽

Essential Oils ◽

Insecticidal Activity ◽

Culex Quinquefasciatus ◽

Citrus Sinensis ◽

Repellent Activity ◽

Syzygium Aromaticum ◽

Dengue Vector ◽

Protection Index ◽

Filarial Vector

Abstract Cymbopogon nardus, Syzygium aromaticum, and Citrus sinensis have been well known to possess insecticidal activity. Here, we investigated the repellent activity of individual of three essential oils (C. nardus, S. aromaticum, C. sinensis) and a combination of essential oils (C. nardus + C. sinensis, C. nardus + S. aromaticum, and C. sinensis + S. aromaticum) against a dengue vector, Aedes aegypti and a filarial vector, Culex quinquefasciatus. The essential oils (1 mg) were smeared evenly on the surface of the left arm (three volunteers) and then subjected to 25 females of A. aegypti and C. quinquefasciatus in the cage. The number of landed mosquitos was observed every hour for 7 hours to determine the protection index (PI). The result showed that individuals of three essential oils and a combination of essential oils generated > 90% of PI until 7 hours of application for both mosquitos species tested. Overall, the data demonstrated that individual of three essential oils, i.e., C. nardus, S. aromaticum, C. sinensis, and various combinations of essential oils (C. nardus + C. sinensis; C. nardus + S. aromaticum; C. sinensis + S. aromaticum) are promising as repellent agents against A. aegypti and C. quinquefasciatus.

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Characterization and Comparison of Five Eucalyptus Essential Oils and their Repellent Activity Against Aedes aegypti

Planta Medica ◽

10.1055/s-0030-1251811 ◽

2010 ◽

Vol 76 (05) ◽

Author(s):

N Tabanca ◽

EK Blythe ◽

UR Bernier ◽

B Demirci ◽

KHC Baser ◽

...

Keyword(s):

Aedes Aegypti ◽

Essential Oils ◽

Repellent Activity

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Repellent activity of selected essential oils against Aedes aegypti

Fitoterapia ◽

10.1016/j.fitote.2007.02.006 ◽

2007 ◽

Vol 78 (5) ◽

pp. 359-364 ◽

Cited By ~ 75

Author(s):

W. Choochote ◽

U. Chaithong ◽

K. Kamsuk ◽

A. Jitpakdi ◽

P. Tippawangkosol ◽

...

Keyword(s):

Aedes Aegypti ◽

Essential Oils ◽

Repellent Activity

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UJI EFEKTIFITAS FORMULA SPRAY DARI MINYAK ATSIRI HERBA KEMANGI (Ocimum Sanctum L ) SEBAGAI REPELLENT NYAMUK Aedes aegypti

Jurnal Ilmiah Manuntung ◽

10.51352/jim.v2i2.66 ◽

2017 ◽

Vol 2 (2) ◽

pp. 189

Author(s):

Resmi Aini ◽

Rina Widiastuti ◽

Nuha Afra Nadhifa

Keyword(s):

Essential Oil ◽

Aedes Aegypti ◽

Essential Oils ◽

Negative Control ◽

Repellent Activity ◽

Ocimum Sanctum ◽

Control Group ◽

Control Group Design ◽

A Value ◽

Post Test

Dengue fever is transmitted by the bite of Aedes aegypti mosquitoes infected with denguevirus. Preparations antinyamuk much use of harmful chemicals such as DEET (diethylmetatoluamide) and permethrin that can lead to pollution of the environment, leavingno residue and cause resistance to the drug. To determine the activity of essential oils from herbs basil (Ocimum Sanctum L) in the preparation of spray as a mosquito repellent and determine the effective concentrationof the spray.This type of research is True experiment with design Post Test Only Control Group Design. Repellent activity was observed by means of spray is sprayed on the back of the hand probandus, then tested on female mosquitoes with the introduction into the cage containing20 mosquitoes each treatment. Spray essential oils showed repellent activity of Aedes aegypti with a value of correlation r = 0.964 higher concentration of essential oils the fewer mosquitoes that land. Spray herbal essential oil of basil (Ocimum basilicum L) has a repellent activitycompared to the negative control. The effectiveness of the herb basil essential oil sprayobtainedat 74.131 ppm lC50 and lC90 of 775.711 ppm

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