In Vitro Characterization and Mosquito (Aedes aegypti) Repellent Activity of Essential-Oils-Loaded Nanoemulsions

ABSTRACT The aim of this work was to evaluate the insecticidal and repellency activity of the essential oil of Cinnamomum camphora var. linaloolifera Y. Fujita (Ho-Sho) and Cinnamomumcamphora (L.) J Presl.var. hosyo (Hon-Sho), against the Sitophilus zeamais in maize grains. The essential oils were obtained by hydrodistillation and analyzed by GC-MS.The insecticidal activity was determined by the toxicity of different concentrations of essential oils during 24 hours of contact with the insects, in the absence of feed substrate. The Bioassays of repellency were conducted with lethal doses (LD50,LD25,and LD12.5) obtained from insecticidal bioassay. In order to compare the treatments the preference index (PI) was employed. The analysis of the essential oils of Cinnamomum camphora leaves indicated 68% of camphor and 9% of linalool for the variation Hon-Sho and 95% of linalool to the variation Ho-Sho. The variation Ho-Sho presented greatest insecticidal activity than the variation Hon-Sho against the Sitophiluszeamais, with LD50 of 0.35 μL/cm2, whereas in the variation Hon-Sho the ratewas 0.48 μL/cm2. However, considering only the concentrations of linalool and camphor of Ho-Sho and Hon-Sho, the lethal doses’ evaluation of these compounds were similar. The values of the preference index ranged from -0.3 to -0.8 for thevariation Ho-Sho and -0.2 to -0.7 for the variation Hon-Sho. The essential oils evaluated in this work showed repellent activity against Sitophiluszeamais in vitro and in trials performed in mini-silos.

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Insecticidal and Biting Deterrent Activities of Magnolia grandiflora Essential Oils and Selected Pure Compounds against Aedes aegypti

Molecules ◽

10.3390/molecules25061359 ◽

2020 ◽

Vol 25 (6) ◽

pp. 1359

Author(s):

Abbas Ali ◽

Nurhayat Tabanca ◽

Betul Demirci ◽

Vijayasankar Raman ◽

Jane M. Budel ◽

...

Keyword(s):

Essential Oil ◽

Aedes Aegypti ◽

Essential Oils ◽

Screening Program ◽

Negative Control ◽

Magnolia Grandiflora ◽

Plant Parts ◽

Pure Compounds ◽

Index Value

In our natural products screening program for mosquitoes, we tested essential oils extracted from different plant parts of Magnolia grandiflora L. for their insecticidal and biting deterrent activities against Aedes aegypti. Biting deterrence of seeds essential oil with biting deterrence index value of 0.89 was similar to N,N-diethyl-3-methylbenzamide (DEET). All the other oils were active above the solvent control but the activity was significantly lower than DEET. Based on GC-MS analysis, three pure compounds that were only present in the essential oil of seed were further investigated to identify the compounds responsible for biting deterrent activity. 1-Decanol with PNB value of 0.8 was similar to DEET (PNB = 0.8), whereas 1-octanol with PNB value of 0.64 showed biting deterrence lower than 1-decanol and DEET. The activity of 1-heptanol with PNB value of 0.36 was similar to the negative control. Since 1-decanol, which was 3.3% of the seed essential oil, showed biting deterrence similar to DEET as a pure compound, this compound might be responsible for the activity of this oil. In in vitro A & K bioassay, 1-decanol with MED value of 6.25 showed higher repellency than DEET (MED = 12.5). Essential oils of immature and mature fruit showed high toxicity whereas leaf, flower, and seeds essential oils gave only 20%, 0%, and 50% mortality, respectively, at the highest dose of 125 ppm. 1-Decanol with LC50 of 4.8 ppm was the most toxic compound.

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Repellent activity of essential oils isolated from the leaves of Eupatorium odoratum against malaria, filaria and dengue vectors

Environment Conservation Journal ◽

10.36953/ecj.2013.141203 ◽

2013 ◽

Vol 14 (1&2) ◽

pp. 21-25

Author(s):

M. F. Alam

Keyword(s):

Essential Oil ◽

Aedes Aegypti ◽

Essential Oils ◽

Anopheles Stephensi ◽

Mosquito Species ◽

Repellent Activity ◽

Complete Protection ◽

Protection Measure ◽

Human Volunteers ◽

Dengue Vectors

Light green essential oils extracted from the leaves of Eupatorium odoratum (Asterace) from Himalaya region (India) were screened for repellent activity against Anopheles stephensi, Aedes aegypti and Culex quinquifaciatus mosquitoes. The repellent activity was evaluated in door condition. Four concentrations 2.5%, 5%, 7.5% and 10% (v/v) in ethanol were considered in this study. When the test oil was applied at 10% concentration were found most effective and provided more than 2hr of complete protection against all three mosquito species. The complete protection time (Mean ± S.D) were recorded as 160.2 ± 2.77min, 139.4 ± 1.14min and 125.6 ± 3.13min against Anopheles stephensi, Aedes aegypti and Culex quinquifaciatus respectively. There was no bite within 125min and 160min confirm due to action of essential oil and thus 100% protection from the bite of mosquitoes achieved. No adverse effects of the human volunteers were observed during the exposure and through three months after the application.Therefore, the essential oil of Eupatorium odoratum (Asterace) may prove useful in the development of mosquito natural repellents as an effective personal protection measure against mosquito bites.

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Chemical Composition and Repellent Activity against Mosquito Aedes Aegypti of Pelargonium radula, Syzygiumaromaticum and Citrus aurantifolia Essential Oils

Materials Science Forum ◽

10.4028/www.scientific.net/msf.981.253 ◽

2020 ◽

Vol 981 ◽

pp. 253-257

Author(s):

Hazrulrizawati Abd Hamid ◽

Nishantini Silvarajoo ◽

Nurulhusna Ab. Hamid

Keyword(s):

Chemical Composition ◽

Essential Oil ◽

Aedes Aegypti ◽

Essential Oils ◽

Dengue Fever ◽

Yellow Fever ◽

Repellent Activity ◽

Mosquito Repellent ◽

Citrus Aurantifolia ◽

Frequent Use

The mosquito Aedes aegypti is an epidemic vector of several diseases such as dengue fever and yellow fever. Several pesticides are used to control the mosquito population. Because of their frequent use, some mosquitoes have developed resistance. In the present study, we evaluated the synergistic mosquito-repellent activity of essential oils from Pelargonium radula, Syzgium aromaticum and Citrus aurantifolia against Aedes aegypti by using Y-tube olfactometer. The oils was subsequently analyzed by using GC–MS. These results clearly reveal that the essential oil of C. aurantifolia served as the most potent repellent agent against Aedes aegypti . The results indicate that three constituents; limonene (19.58%) followed by β–pinene (17.12%), geraniol (13.23%) which comprise a large proportion of the C. aurantifolia are likely responsible for the observed repellent activity.

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IN VITRO CHARACTERIZATION OF ANTIFUNGAL ACTIVITY OF SELECTED ESSENTIAL OILS

INDIAN DRUGS ◽

10.53879/id.57.12.12466 ◽

2021 ◽

Vol 57 (12) ◽

pp. 61-64

Author(s):

C. Rath Chandi ◽

A. Samal

Keyword(s):

Antifungal Activity ◽

Essential Oils ◽

Inhibitory Concentration ◽

Inhibition Mechanism ◽

Active Components ◽

Irreversible Damage ◽

Antifungal Activities ◽

In Vitro Characterization

Antifungal activity of seven essential oils was studied primarily against C. albicans and E. floccosum. Four essential oils viz. turmeric, palmarosa, lemongrass and citronella, that revealed better antifungal activities during screening, were characterized further. Minimum Inhibitory concentration (MIC) and phenol coefficient values of the oils ranged between 0.97 to 62.5 µl/ml and 0125 to 0.75 respectively. The oils retained the antifungal activities when treated at high temperature (1000 C for 1h) and pressure (autoclaved), indicating presence of thermostable and barostable active components in them. The oils also resisted sonication (33,000Hz for 30min) and revealed antifungal activities against the two pathogens. Immediate killing of E. floccosum, is attributable to an irreversible damage to the cells. Antifungal activity of these oils as recorded during the study, could be attributable to the membrane inhibition mechanism and was observed to be fungicidal in nature.

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An in vitro ULV olfactory bioassay method for testing the repellent activity of essential oils against moths

MethodsX ◽

10.1016/j.mex.2018.03.012 ◽

2018 ◽

Vol 5 ◽

pp. 375-394

Author(s):

Petros T. Damos

Keyword(s):

Essential Oils ◽

Repellent Activity ◽

Bioassay Method

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Repellent activity of three essential oils against dengue and filarial vector mosquitoes

IOP Conference Series Earth and Environmental Science ◽

10.1088/1755-1315/918/1/012001 ◽

2021 ◽

Vol 918 (1) ◽

pp. 012001

Author(s):

D Meisyara ◽

D Tarmadi ◽

A Zulfitri ◽

A Fajar ◽

S K Himmi ◽

...

Keyword(s):

Aedes Aegypti ◽

Essential Oils ◽

Insecticidal Activity ◽

Culex Quinquefasciatus ◽

Citrus Sinensis ◽

Repellent Activity ◽

Syzygium Aromaticum ◽

Dengue Vector ◽

Protection Index ◽

Filarial Vector

Abstract Cymbopogon nardus, Syzygium aromaticum, and Citrus sinensis have been well known to possess insecticidal activity. Here, we investigated the repellent activity of individual of three essential oils (C. nardus, S. aromaticum, C. sinensis) and a combination of essential oils (C. nardus + C. sinensis, C. nardus + S. aromaticum, and C. sinensis + S. aromaticum) against a dengue vector, Aedes aegypti and a filarial vector, Culex quinquefasciatus. The essential oils (1 mg) were smeared evenly on the surface of the left arm (three volunteers) and then subjected to 25 females of A. aegypti and C. quinquefasciatus in the cage. The number of landed mosquitos was observed every hour for 7 hours to determine the protection index (PI). The result showed that individuals of three essential oils and a combination of essential oils generated > 90% of PI until 7 hours of application for both mosquitos species tested. Overall, the data demonstrated that individual of three essential oils, i.e., C. nardus, S. aromaticum, C. sinensis, and various combinations of essential oils (C. nardus + C. sinensis; C. nardus + S. aromaticum; C. sinensis + S. aromaticum) are promising as repellent agents against A. aegypti and C. quinquefasciatus.

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