Changes of oxidative stress markers after treatment of diabetic neuropathy with alpha-lipoic acid

AbstractAlpha lipoic acid is an antioxidant substance used for the pathogenic treatment of diabetic neuropathy, oxidative stress being a central mechanism in diabetic microvascular complications. Our study included 24 diabetes mellitus patients with diabetic neuropathy and 20 healthy subjects. Diabetes patients were given alpha lipoic acid 600 mg intravenously for 10 days and then per os for 30 days.Significant improvements were observed concerning oxidative stress evaluated by measuring serum malondyaldehide and ceruloplasmin. The clinical characteristic of neuropathy improved, both the level of pain decreased and the vibration perception threshold increased. Our study demonstrated a two times higher level of oxidative stress in patients with diabetes compared to healthy subjects, and that by influencing oxidative stress we could influence the clinical aspects of neuropathy. Further investigations need to be done to explore the pleiotropic effects of alpha lipoic acid on other mechanisms that are implicated in the pathogenies of diabetic neuropathy.

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Lipoic acid and moderate swimming improves the estrous cycle and oxidative stress in Wistar rats

Applied Physiology Nutrition and Metabolism ◽

10.1139/h11-074 ◽

2011 ◽

Vol 36 (5) ◽

pp. 693-697 ◽

Cited By ~ 8

Author(s):

Rand Randall Martins ◽

Ulisvaldo Brunno de Oliveira Macedo ◽

Lúcia Dantas Leite ◽

Adriana Augusto Rezende ◽

José Brandão-Neto ◽

...

Keyword(s):

Oxidative Stress ◽

Estrous Cycle ◽

Antioxidant System ◽

Lipoic Acid ◽

Wistar Rats ◽

Cycle Duration ◽

Oxidative Stress Markers ◽

Stress Markers ◽

Group 4 ◽

Group 2

The generation of reactive oxygen species resulting from physical activity may trigger adaptive processes at the reproductive level and in the antioxidant defense system itself. The objective of this study was to investigate the effects of moderate daily swimming and lipoic acid (LA) supplementation on estrous cycle duration and pro-oxident and antioxidant markers in young Wistar rats. Animals were submitted to daily swimming (for 1 h) for 30 days, between 1300 h and 1400 h. The following study groups were formed: group 1, sedentary; group 2, submitted to swimming; group 3, sedentary supplemented with 100 mg·kg–1·day–1 of LA; and group 4, submitted to swimming and supplementation with 100 mg·kg–1·day–1 of LA. The estrous cycle of the animals was evaluated daily, and the following oxidative stress markers were measured: plasma thiobarbituric acid reactive substances (TBARS) and glutathione (GSH), erythrocyte superoxide dismutase (SOD), GSH peroxidase (GPx) and catalase (CAT) activity. The exercise protocol increased estrous cycle duration in group 2, especially in the diestrous phase. There was also a decrease in lipoperoxidation, with enhanced antioxidant activity of SOD and GPx. Group 4 showed no alteration in estrous cycle duration and maintained the beneficial effects on the antioxidant system observed in group 2. The increase in estrous cycle duration and improved oxidative stress markers may be an adaptive response to moderate exercise. LA impeded any exercise-induced alteration in the cycle but preserved improvements in the antioxidant system.

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Oxidative stress markers in cognitively intact patients with diabetic neuropathy

Brain Research Bulletin ◽

10.1016/j.brainresbull.2019.06.001 ◽

2019 ◽

Vol 150 ◽

pp. 196-200 ◽

Cited By ~ 3

Author(s):

Isaac Etienne ◽

Lucas Vilas Boas Magalhães ◽

Silvia Almeida Cardoso ◽

Rodrigo Barros de Freitas ◽

Guilherme Pereira de Oliveira ◽

...

Keyword(s):

Oxidative Stress ◽

Diabetic Neuropathy ◽

Oxidative Stress Markers ◽

Stress Markers ◽

Cognitively Intact

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Association of Oxidative Stress Markers with Vascular Stiffness Parameters in Patients with Diabetic Neuropathy

BioMed ◽

10.3390/biomed2010001 ◽

2021 ◽

Vol 2 (1) ◽

pp. 1-12

Author(s):

Dominika Mačáková ◽

Markéta Plchová ◽

Lubica Cibičková ◽

Ondřej Krystyník ◽

David Karásek ◽

...

Keyword(s):

Oxidative Stress ◽

Diabetic Neuropathy ◽

Lipoic Acid ◽

Vascular Stiffness ◽

Infusion Therapy ◽

Control Group ◽

Alpha Lipoic Acid ◽

Complications Of Diabetes ◽

Oxidative Markers ◽

Wall Stiffness

Introduction: One of the most common chronic complications of diabetes mellitus is diabetic neuropathy. The aim of the study was to elucidate the association between selected markers of oxidative stress and markers of vascular stiffness and to contribute to the understanding of the pathophysiological links between oxidative stress and micro- and macrovascular complications of diabetes. Methods: We enrolled patients with type 2 DM (n = 49), with moderate to severe diabetic polyneuropathy of lower extremities, and a control group without microvascular complications (n = 29). The neuropathy group received alpha-lipoic acid infusion therapy. Sampling was performed before and after treatment to determine the level of oxidative markers (advanced glycation end-products—AGEs, glycation products of AOPP proteins, MDA malondialdehyde and oxidized LDL), parameters of metabolic control and parameters of vascular wall stiffness were measured by sphygmomanometry. Results: After the administration of alpha-lipoic acid, we demonstrated a significant reduction in the level of three selected oxidation markers (AOPP: p < 0.001, AGE: p < 0.001, oxLDL: p < 0.05). In contrast, the level of MDA did not change significantly (p = 0.83). Throughout the group, oxLDL was significantly correlated with central BP (SBP and DBP in the aorta, p < 0.05 and <0.01) and with the augmentation index (AiX/75 bpm, p < 0.01). AOPP significantly correlated with systolic BP in the aorta (p < 0.05). We did not find significant associations in the remaining oxidation markers. Conclusion: In our study, we demonstrated a reduction in the level of oxidative markers after alpha-lipoic acid administration and also an association between markers of oxidative damage to lipids and proteins (oxLDL and AOPP) and some parameters of vascular stiffness.

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