Human normal T lymphocytes and lymphoid cell lines do express alternative splicing variants of human telomerase reverse transcriptase (hTERT) mRNA

2007 ◽  
Vol 353 (4) ◽  
pp. 999-1003 ◽  
Author(s):  
Marit Jalink ◽  
Zheng Ge ◽  
Cheng Liu ◽  
Magnus Björkholm ◽  
Astrid Gruber ◽  
...  
Keyword(s):  
Alternative Splicing ◽  
T Lymphocytes ◽  
Reverse Transcriptase ◽  
Lymphoid Cell ◽  
Telomerase Reverse Transcriptase ◽  
Htert Mrna ◽  
Human Telomerase Reverse Transcriptase ◽  
Splicing Variants ◽  
Human Telomerase ◽  
Lymphoid Cell Lines

Identification of human telomerase reverse transcriptase–derived peptides that induce HLA-A24–restricted antileukemia cytotoxic T lymphocytes

Blood ◽  
2001 ◽  
Vol 97 (9) ◽  
pp. 2903-2907 ◽  
Author(s):  
Junko Arai ◽  
Masaki Yasukawa ◽  
Hideki Ohminami ◽  
Miki Kakimoto ◽  
Atsuhiko Hasegawa ◽  
...  
Keyword(s):  
T Lymphocytes ◽  
Reverse Transcriptase ◽  
Cytotoxic T Lymphocytes ◽  
Leukemia Cells ◽  
Telomerase Reverse Transcriptase ◽  
Malignant Cells ◽  
Human Telomerase Reverse Transcriptase ◽  
European Descent ◽  
Ctl Epitopes ◽  
Human Telomerase

Abstract Human telomerase reverse transcriptase (hTERT) is considered a potential target for cancer immunotherapy because it is preferentially expressed in malignant cells. hTERT-derived peptides carrying motifs for HLA-A24 (HLA-A*2402), the most common allele among Japanese and also frequently present in persons of European descent, were examined for their capacity to elicit antileukemia cytotoxic T lymphocytes (CTLs). Two of the 5 peptides tested, VYAETKHFL and VYGFVRACL, appeared capable of generating hTERT peptide-specific and HLA-A24–restricted CTLs. The CD8+ CTL clones specific for these hTERT peptides exerted cytotoxicity against leukemia cells in an HLA-A24–restricted manner. This cytotoxicity was inhibited by the addition of hTERT peptide-loaded autologous cells, suggesting that hTERT is naturally processed in leukemia cells and that hTERT-derived peptides are expressed on these cells and are recognized by CTLs in the context of HLA-A24. Taken together with the currently identified HLA-A2–restricted CTL epitopes derived from hTERT, identification of new CTL epitopes presented by HLA-A24 increases the feasibility of immunotherapy for leukemia using hTERT-derived peptides.

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