Co-culture with Sertoli cells promotes proliferation and migration of umbilical cord mesenchymal stem cells

Umbilical cord mesenchymal stem cells (UC-MSCs) are a class of multifunctional stem cells isolated and cultured from umbilical cord. They possessed the characteristics of highly self-renewal, multi-directional differentiation potential and low immunogenicity. Its application in the field of tissue engineering and gene therapy has achieved a series of results. Recent studies have confirmed their characteristics of inhibiting tumor cell proliferation and migration to nest of cancer. The ability of UC-MSCs to support hematopoietic microenvironment and suppress immune system suggests that they can improve engraftment after hematopoietic stem cell transplantation, which shows great potential in treatment of hematologic diseases. This review will focus on the latest advances in biological characteristics and mechanism of UC-MSCs in treatment of hematological diseases.

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Umbilical cord-derived mesenchymal stem cells promote proliferation and migration in MCF-7 and MDA-MB-231 breast cancer cells through activation of the ERK pathway

Oncology Reports ◽

10.3892/or.2015.4109 ◽

2015 ◽

Vol 34 (3) ◽

pp. 1469-1477 ◽

Cited By ~ 26

Author(s):

TAO LI ◽

CHUNFU ZHANG ◽

YANLING DING ◽

WEI ZHAI ◽

KUI LIU ◽

...

Keyword(s):

Breast Cancer ◽

Stem Cells ◽

Mesenchymal Stem Cells ◽

Cancer Cells ◽

Umbilical Cord ◽

Breast Cancer Cells ◽

Erk Pathway ◽

And Migration ◽

Mcf 7 ◽

Proliferation And Migration

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Hydroxyapatite-Based Colloidal Gels Facilitate the Proliferation and Migration of Chondrocytes and the Adhesion of Umbilical Cord Mesenchymal Stem Cells

International Scholarly Research Notices ◽

10.1155/2014/935689 ◽

2014 ◽

Vol 2014 ◽

pp. 1-7

Author(s):

Syed A. Jamal ◽

Qiang Ye

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Umbilical Cord ◽

Glycolic Acid ◽

Human Umbilical Cord ◽

Colloidal Gels ◽

Unidirectional Movement ◽

Colloidal Gel ◽

And Migration ◽

Proliferation And Migration

Collective movement of cells that have been delivered on biomaterials for transplantation purposes would be a desirable attribute that would promote wound healing, cell proliferation, and eventual growth and regeneration of damaged organs. We hypothesized that colloidal gels made from hydroxyapatite (HA) and poly(D,L-lactic-co-glycolic acid) (PLGA) particles will be conducive to the growth and migration of porcine chondrocytes, will allow the adhesion of human umbilical cord mesenchymal stem cells, and will have negligible effects on the cell cycle of these cells. Then, we performed experiments designed to assess the viability and migratory properties of porcine chondrocytes studded on nanosized HA/PLGA particles. Our experiments show that porcine chondrocytes migrated in and around a hydroxyapatite-based biomaterial that could be described as a colloidal gel. Cells in the colloidal gel demonstrated unidirectional movement. Cells were seen to be extending lamellae and were followed by other cells.

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Defined serum‐free three‐dimensional culture of umbilical cord‐derived mesenchymal stem cells yields exosomes that promote fibroblast proliferation and migration in vitro

The FASEB Journal ◽

10.1096/fj.202001768rr ◽

2020 ◽

Vol 35 (1) ◽

Author(s):

Farid N. Faruqu ◽

Revadee Liam‐Or ◽

Shuai Zhou ◽

Rebecca Nip ◽

Khuloud T. Al‐Jamal

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Umbilical Cord ◽

Three Dimensional ◽

Fibroblast Proliferation ◽

Serum Free ◽

Three Dimensional Culture ◽

And Migration ◽

Proliferation And Migration

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Conditioned medium from the human umbilical cord-mesenchymal stem cells stimulate the proliferation of human keratinocytes

Journal of Basic and Clinical Physiology and Pharmacology ◽

10.1515/jbcpp-2019-0283 ◽

2020 ◽

Vol 0 (0) ◽

Author(s):

Sushmitha Sriramulu ◽

Antara Banerjee ◽

Ganesan Jothimani ◽

Surajit Pathak

Keyword(s):

Stem Cells ◽

Cell Proliferation ◽

Wound Healing ◽

Mesenchymal Stem Cells ◽

Conditioned Medium ◽

Umbilical Cord ◽

Human Keratinocytes ◽

Human Umbilical Cord ◽

Hacat Cells ◽

And Migration

AbstractObjectivesWound healing is a complex process with a sequence of restoring and inhibition events such as cell proliferation, differentiation, migration as well as adhesion. Mesenchymal stem cells (MSC) derived conditioned medium (CM) has potent therapeutic functions and promotes cell proliferation, anti-oxidant, immunosuppressive, and anti-apoptotic effects. The main aim of this research is to study the role of human umbilical cord-mesenchymal stem cells (UC-MSCs) derived CM in stimulating the proliferation of human keratinocytes (HaCaT).MethodsFirstly, MSC were isolated from human umbilical cords (UC) and the cells were then cultured in proliferative medium. We prepared and collected the CM after 72 h. Morphological changes were observed after the treatment of HaCaT cells with CM. To validate the findings, proliferation rate, clonal efficiency and also gene expression studies were performed.ResultsIncreased proliferation rate was observed and confirmed with the expression of Proliferating Cell Nuclear Antigen (PCNA) after treatment with HaCaT cells. Cell-cell strap formation was also observed when HaCaT cells were treated with CM for a period of 5–6 days which was confirmed by the increased expression of Collagen Type 1 Alpha 1 chain (Col1A1).ConclusionsOur results from present study depicts that the secretory components in the CM might play a significant role by interacting with keratinocytes to promote proliferation and migration. Thus, the CM stimulates cellular proliferation, epithelialization and migration of skin cells which might be the future promising application in wound healing.

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