Proteomic profiling of a mouse model of acute intestinal Apc deletion leads to identification of potential novel biomarkers of human colorectal cancer (CRC)

2013 ◽  
Vol 440 (3) ◽  
pp. 364-370 ◽  
Author(s):  
Abeer Hammoudi ◽  
Fei Song ◽  
Karen R. Reed ◽  
Rosalind E. Jenkins ◽  
Valerie S. Meniel ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Mouse Model ◽  
Proteomic Profiling ◽  
Human Colorectal Cancer ◽  
Novel Biomarkers

scholarly journals Protein Expression Profiling Identifies Key Proteins and Pathways Involved in Growth Inhibitory Effects Exerted by Guggulsterone in Human Colorectal Cancer Cells

Cancers ◽  
2019 ◽  
Vol 11 (10) ◽  
pp. 1478 ◽  
Author(s):  
Leo ◽  
Therachiyil ◽  
Siveen ◽  
Uddin ◽  
Kulinski ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Cancer Cells ◽  
P53 Protein ◽  
Colorectal Cancer Cell Line ◽  
Proteomic Profiling ◽  
Human Colorectal Cancer ◽  
Altered Expression ◽  
Apoptosis Pathway ◽  
Intrinsic Apoptosis Pathway ◽  
Hct 116

Colorectal cancer (CRC) is a leading killer cancer worldwide and one of the most common malignancies with increasing incidences of mortality. Guggulsterone (GS) is a plant sterol used for treatment of various ailments such as obesity, hyperlipidemia, diabetes, and arthritis. In the current study, anti-cancer effects of GS in human colorectal cancer cell line HCT 116 was tested, potential targets identified using mass spectrometry-based label-free shotgun proteomics approach and key pathways validated by proteome profiler antibody arrays. Comprehensive proteomic profiling identified 14 proteins as significantly dysregulated. Proteins involved in cell proliferation/migration, tumorigenesis, cell growth, metabolism, and DNA replication were downregulated while the protein with functional role in exocytosis/tumor suppression was found to be upregulated. Our study evidenced that GS treatment altered expression of Bcl-2 mediated the mitochondrial release of cytochrome c which triggered the formation of apoptosome as well as activation of caspase-3/7 leading to death of HCT 116 cells via intrinsic apoptosis pathway. GS treatment also induced expression of p53 protein while p21 expression was unaltered with no cell cycle arrest. In addition, GS was found to inhibit NF-kB signaling in colon cancer cells by quelling the expression of its regulated gene products Bcl-2, cIAP-1, and survivin.

Curcumin sensitizes human colorectal cancer to capecitabine by modulation of cyclin D1, COX‐2, MMP‐9, VEGF and CXCR4 expression in an orthotopic mouse model

2009 ◽  
Vol 125 (9) ◽  
pp. 2187-2197 ◽  
Author(s):  
Ajaikumar B. Kunnumakkara ◽  
Parmeswaran Diagaradjane ◽  
Preetha Anand ◽  
Harikumar B. Kuzhuvelil ◽  
Amit Deorukhkar ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Mouse Model ◽  
Cyclin D1 ◽  
Cxcr4 Expression ◽  
Human Colorectal Cancer ◽  
Orthotopic Mouse Model ◽  
Cox 2
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