Circadian misalignment leads to changes in cortisol rhythms, blood biochemical variables and serum miRNA profiles

Abstract Background: Hucul horses are the unique, genetically distinct breed of Carpathian Mountains. Even though they are recognized as primitive breed, many morphological differences between them and other primitive horses have been reported. Neither hematological nor blood biochemical studies in this breed have been conducted so far. Objectives: The aim of this study was to establish the reference intervals for basic hematological and selected biochemical variables and to compare them with other breeds. Material and Methods: Blood samples were collected from 168 Hucul horses and the analyses were performed using routine methods. Mainly nonparametric method was used to establish reference intervals. Results: The following reference intervals have been established (rounded to two significant digits): RBC: 7.0-13×1012/l; HGB: 106.1-195.8 g/l; HCT: 0.3-0.6 l/l; MCV: 35-50 fl; MCH 11.9-17.1 pg; MCHC: 31.9-34.8 g/dl; WBC: 7.5-22×109/l, bands: 0-0.5×109/l; segmented neutrophils: 3.3-10×109/l; eosinophils: 0-1.1×109/l; basophils: 0-0.3×109/l; lymphocytes: 1.9-12×109/l; monocytes: 0-0.2×109/l; PLT 95-350×109/l; MPV 5.2-7.0; ALP: 98-425 U/l; AST: 220-470 U/l; GGT: 9.1-31 U/l; total bilirubin: 6.5-29 μmol/l; CPK: 120-640 U/l; triglycerides: 0.1-0.9 mmol/l; urea: 3.8-11 mmol/l; creatinine: 44 -140 μmol/l; serum amyloid A: 130-5200 μg/l. Conclusions: Hematological and biochemical variables in Hucul horses were closer to hot-blooded then to cold-blooded and primitive horses or wild equidae. The reference intervals presented in this study pose clinically useful tool for evaluation of blood check-up in Hucul horses.

Download Full-text

REFERENCE INTERVAL OF BLOOD BIOCHEMICAL VARIABLES IN RUSTIPOLLOS RAISED UNDER CONDITIONS OF SUBTROPICAL-HUMID CLIMATE

Compendio de Ciencias Veterinarias ◽

10.18004/compend.cienc.vet.2018.08.02.13-19 ◽

2018 ◽

Vol 8 (2) ◽

pp. 13-19

Author(s):

L Castro ◽

M Méndez ◽

G Gómez ◽

R Pedrozo

Keyword(s):

Reference Interval ◽

Humid Climate ◽

Biochemical Variables ◽

Blood Biochemical

Download Full-text

Effects of trailer transport duration on body weight and blood biochemical variables of horses

Pferdeheilkunde Equine Medicine ◽

10.21836/pem19960413 ◽

1996 ◽

Vol 12 (4) ◽

pp. 435-437 ◽

Cited By ~ 8

Author(s):

M A Foss ◽

A Lindner

Keyword(s):

Body Weight ◽

Biochemical Variables ◽

Blood Biochemical

Download Full-text

Disturbance of Circadian Rhythms Extensively Affects Blood Biochemical Parameters and Serum miRNA Profiles

10.21203/rs.3.rs-95425/v1 ◽

2020 ◽

Author(s):

Guo-Bin Huang ◽

Huan Ma ◽

Xi-Hui Gan ◽

Si-Lin Li ◽

Xiao-Hong Ma ◽

...

Keyword(s):

Circadian Rhythms ◽

Blood Serum ◽

Biochemical Variables ◽

Blood Biochemical ◽

Physiological Processes ◽

Serum Mirnas ◽

Increased Risk ◽

Shift Schedules ◽

Serum Biochemical ◽

And Performance

Abstract Background: Circadian clock plays a critical role in synchronizing the inner molecular, metabolic and physiological processes to the environmental cues with a period of 24 h. Misalignment in the circadian rhythms leads to decreased adaptation and performance and increased risk of associated disorders. The non-24-h schedules and shift schedules are commonly used in maritime operations, both of which could result in disturbance of circadian rhythms.Methods: In this study, we recruited volunteers and conducted two experiments: in one experiment 15 subjects followed an 8-h on and 4-h off schedule (non-24-h schedule), and in the second experiment 12 subjects followed a 3-d rotary schedule with consecutive shift in sleep time (phase-changing schedule). The serum/blood biochemical variables were measured and the serum miRNAs of the volunteers in the second experiment were subjected to transcriptomic miRNA sequencing.Results: The results show both of the schedules caused comprehensive changes in the blood/serum biochemical variables. Notably, significant elevation in serum phosphate was observed in both experiments: 1.210 ± 0.141 in control and 1.330 ± 0.117 in recovery (P = 0.014) in the first experiment and 1.193 ± 0.152 in control and 1.343 ± 0.099 in recovery (P = 0.007) in the second experiment. In addition, a subset of serum miRNAs targeting genes involved in circadian rhythms, sleep homeostasis, phosphate metabolism and multiple critical physiological processes or pathways were identified in the second experiments.Conclusions: This study reveals that non-24-h and shift schedules lead to changes in a large spectrum of blood/serum biochemical variables due to circadian misalignment. Schedules with frequent shift may cause remarkable changes in serum miRNAs which are involved in multiple physiological pathways. These findings would help understand the deleterious effects of shift schedules and develop optimized strategy to enhance welfare and performance of the shift workers.

Download Full-text

Effects of age and sex on blood biochemistry of dorper lambs

Semina Ciências Agrárias ◽

10.5433/1679-0359.2017v38n5p3085 ◽

2017 ◽

Vol 38 (5) ◽

pp. 3085 ◽

Cited By ~ 1

Author(s):

Rebecca Espírito Santo da Cruz ◽

Fernando Melo Rocha ◽

Cassius Vinícius Barbosa Sena ◽

Pablo Gomes Noleto ◽

Ednaldo Carvalho Guimarães ◽

...

Keyword(s):

Age Groups ◽

Gamma Glutamyl Transferase ◽

Low Density ◽

Biochemical Variables ◽

Blood Biochemical ◽

Significant Difference ◽

Calcium Phosphorus ◽

Dorper Sheep ◽

Glutamyl Transferase ◽

High Density Cholesterol

The purpose of this study was to evaluate the physiological variations and influence of age group and sex on biochemical variables of 32 male and female Dorper sheep at the age of 15 to 121 days. Four blood samples were collected from each animal, at monthly intervals, totaling 128 samples. Biochemical variables determined in each sample were total protein (TP), albumin (ALB), globulin (GLB), albumin/globulin ratio (A/G), glucose (GLU), triglycerides (TG), total cholesterol (tCHOL), high density cholesterol carrying lipoproteins (HDL-C), low density cholesterol carrying lipoproteins (LDL-C), very low density cholesterol carrying lipoproteins (VLDL-C), urea, creatinine (CRE), total calcium (tCa), ionized calcium (iCa), phosphorus (P), calcium/phosphorus ratio (Ca/P), magnesium (Mg), sodium (Na), potassium (K), chloride (Cl), alanine aminotransferase (ALT), aspartate aminotransferase (AST), gamma-glutamyl transferase (GGT) and alkaline phosphatase (ALP). The results were obtained in four moments according to age: M1 - sheep from 15 to 30 days, M2 - sheep from 45 to 61 days, M3 - sheep from 74 to 90 days and M4 - sheep from 105 to 121 days. Comparing the values of the biochemical variables among the four age groups studied a significant difference (p < 0.05) was observed in the concentrations of all the variables evaluated except for Na, Cl and ALT. Sex did not influence (p > 0.05) the values of the biochemical variables analyzed. Based on the results, it is concluded that the age groups exert a significant influence on the majority of blood biochemical variables of Dorper sheep from 15 to 121 days of age.

Download Full-text