Reference intervals for selected hematological and biochemical variables in Hucul horses

Abstract Background: Hucul horses are the unique, genetically distinct breed of Carpathian Mountains. Even though they are recognized as primitive breed, many morphological differences between them and other primitive horses have been reported. Neither hematological nor blood biochemical studies in this breed have been conducted so far. Objectives: The aim of this study was to establish the reference intervals for basic hematological and selected biochemical variables and to compare them with other breeds. Material and Methods: Blood samples were collected from 168 Hucul horses and the analyses were performed using routine methods. Mainly nonparametric method was used to establish reference intervals. Results: The following reference intervals have been established (rounded to two significant digits): RBC: 7.0-13×1012/l; HGB: 106.1-195.8 g/l; HCT: 0.3-0.6 l/l; MCV: 35-50 fl; MCH 11.9-17.1 pg; MCHC: 31.9-34.8 g/dl; WBC: 7.5-22×109/l, bands: 0-0.5×109/l; segmented neutrophils: 3.3-10×109/l; eosinophils: 0-1.1×109/l; basophils: 0-0.3×109/l; lymphocytes: 1.9-12×109/l; monocytes: 0-0.2×109/l; PLT 95-350×109/l; MPV 5.2-7.0; ALP: 98-425 U/l; AST: 220-470 U/l; GGT: 9.1-31 U/l; total bilirubin: 6.5-29 μmol/l; CPK: 120-640 U/l; triglycerides: 0.1-0.9 mmol/l; urea: 3.8-11 mmol/l; creatinine: 44 -140 μmol/l; serum amyloid A: 130-5200 μg/l. Conclusions: Hematological and biochemical variables in Hucul horses were closer to hot-blooded then to cold-blooded and primitive horses or wild equidae. The reference intervals presented in this study pose clinically useful tool for evaluation of blood check-up in Hucul horses.

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Therapeutic Effects of Mutian® Xraphconn on 141 Client-Owned Cats with Feline Infectious Peritonitis Predicted by Total Bilirubin Levels

Veterinary Sciences ◽

10.3390/vetsci8120328 ◽

2021 ◽

Vol 8 (12) ◽

pp. 328

Author(s):

Masato Katayama ◽

Yukina Uemura

Keyword(s):

Serum Amyloid A ◽

Total Bilirubin ◽

Viral Gene Expression ◽

Viral Gene ◽

Fatal Disease ◽

Feline Infectious Peritonitis ◽

Acid Glycoprotein ◽

Amyloid A ◽

Survivor Group ◽

Pre Treatment

Feline infectious peritonitis (FIP) is a fatal disease caused by feline coronavirus or its variant, referred to as the FIP virus. Recently, favorable treatment outcomes of the anti-viral drug Mutian® Xraphconn (Mutian X) were noted in cats with FIP. Thus, the therapeutic efficacy of Mutian X in cats with FIP must be explored, although the predictors of therapeutic success remain unknown. In the present study, we administered Mutian X to 141 pet cats with effusive FIP following initial veterinarian examinations. Of these, 116 cats survived but the remaining 25 died during treatment. Pre-treatment signalment, viral gene expression, and representative laboratory parameters for routine FIP diagnosis (i.e., hematocrit, albumin-to-globulin ratio, total bilirubin, serum amyloid-A, and α1-acid glycoprotein) were statistically compared between the survivor and non-survivor groups. The majority of these parameters, including hematocrit, albumin-to-globulin ratio, serum amyloid-A, α1-acid glycoprotein, and viral gene expression, were comparable between the two groups. Interestingly, however, total bilirubin levels in the survivor group were significantly lower than those in the non-survivor group (p < 0.0001). Furthermore, in almost all surviving cats with effusive FIP (96.6%, 28/29), the pre-treatment total bilirubin levels were below 0.5 mg/dL; however, the survival rate decreased drastically (14.3%, 1/7) when the pre-treatment total bilirubin levels exceeded 4.0 mg/dL. Thus, circulating total bilirubin levels may act as a prognostic risk factor for severe FIP and may serve as the predictor of the therapeutic efficacy of Mutian X against this fatal disease.

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Health reference intervals and values for common bottlenose dolphins (Tursiops truncatus), Indo-Pacific bottlenose dolphins (Tursiops aduncus), Pacific white-sided dolphins (Lagenorhynchus obliquidens), and beluga whales (Delphinapterus leucas)

PLoS ONE ◽

10.1371/journal.pone.0250332 ◽

2021 ◽

Vol 16 (8) ◽

pp. e0250332

Author(s):

Lisa K. Lauderdale ◽

Michael T. Walsh ◽

Kevin A. Mitchell ◽

Douglas A. Granger ◽

Jill D. Mellen ◽

...

Keyword(s):

Tursiops Truncatus ◽

Bottlenose Dolphins ◽

Reference Intervals ◽

Least Square ◽

Delphinapterus Leucas ◽

Blood Samples ◽

Tursiops Aduncus ◽

Beluga Whales ◽

Biochemical Variables ◽

Lagenorhynchus Obliquidens

This study reports comprehensive clinical pathology data for hematology, serum, and plasma biochemistry reference intervals for 174 apparently healthy common bottlenose dolphins (Tursiops truncatus) and reference values for 27 Indo-Pacific bottlenose dolphins (Tursiops aduncus), 13 beluga whales (Delphinapterus leucas), and 6 Pacific white-sided dolphins (Lagenorhynchus obliquidens) in zoos and aquariums accredited by the Alliance for Marine Mammal Parks and Aquariums and the Association of Zoos & Aquariums. Blood samples were collected as part of a larger study titled “Towards understanding the welfare of cetaceans in zoos and aquariums” (colloquially called the Cetacean Welfare Study). Two blood samples were collected following a standardized protocol, and two veterinarian examinations were conducted approximately six months apart between July to November 2018 and January to April 2019. Least square means, standard deviations, and 95% confidence intervals were calculated for hematology, serum, and plasma biochemical variables. Comparisons by age, gender, and month revealed statistically significant differences (p < 0.01) for several variables. Reference intervals and values were generated for samples tested at two laboratories for up to 56 hematologic, serum, and plasma biochemical variables. To apply these data, ZooPhysioTrak, an iOS mobile software application, was developed to provide a new resource for cetacean management. ZooPhysioTrak provides species-specific reference intervals and values based on user inputs of individual demographic and sample information. These data provide a baseline from which to compare hematological, serum, and plasma biochemical values in cetaceans in zoos and aquariums.

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Targeted mass spectrometry for Serum Amyloid A (SAA) isoform profiling in sequential blood samples from experimentally Staphylococcus aureus infected pigs

Journal of Proteomics ◽

10.1016/j.jprot.2020.103904 ◽

2020 ◽

Vol 227 ◽

pp. 103904

Author(s):

Anna Barslund Leuchsenring ◽

Christofer Karlsson ◽

Louise Bundgaard ◽

Johan Malmström ◽

Peter M.H. Heegaard

Keyword(s):

Mass Spectrometry ◽

Staphylococcus Aureus ◽

Serum Amyloid A ◽

Serum Amyloid ◽

Blood Samples ◽

Amyloid A

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Comparison of Measurements of 12 Analytes in Equine Blood Samples Using the In-Practice Falcor 350 and the Reference KoneLab 30i Analysers

ISRN Veterinary Science ◽

10.5402/2012/475419 ◽

2012 ◽

Vol 2012 ◽

pp. 1-5 ◽

Cited By ~ 1

Author(s):

K. Papasouliotis ◽

K. V. Tennant ◽

S. Dodkin ◽

J. Mason

Keyword(s):

Alkaline Phosphatase ◽

Creatine Kinase ◽

Aspartate Aminotransferase ◽

Total Bilirubin ◽

Reference Intervals ◽

Total Calcium ◽

Total Proteins ◽

Blood Samples ◽

Additional Correction ◽

Sodium And Potassium

Falcor 350 is a wet-reagent biochemistry analyser that is available for in-house use. The aim of this study was to compare the results produced by this analyser with those obtained by the KoneLab 30i that served as the reference instrument. Blood samples from 60 clinical cases were analysed for urea, creatinine, total proteins, albumin, creatine kinase, aspartate aminotransferase, alkaline phosphatase, total bilirubin, total calcium, phosphate, sodium, and potassium using both instruments. Good to excellent correlations (rs value) value) were identified for creatinine (0.88), total proteins (0.92), albumin (0.93), creatine kinase (0.98), aspartate aminotransferase (0.98), alkaline phosphatase (0.94), total bilirubin (0.98), phosphate (0.95), and potassium (0.97). The correlations for total calcium (0.71), sodium (0.68), and urea (0.64) were fair. For albumin, aspartate aminotransferase, creatine kinase, phosphate, potassium, total bilirubin, creatinine, and total proteins, the two instruments produce values that are closely related to each other and are sufficiently similar to allow them to be used interchangeably without the need for additional correction factor computations. Because of differences in the methodologies, the Falcor results for alkaline phosphatase, total calcium, and sodium cannot be used interchangeably and should be interpreted using reference intervals established from the Falcor analyser.

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Pharmacological Therapy of 141 Client-Owned Cats with Feline Infectious Peritonitis with Mutian ® Xraphconn Adenosine Nucleoside Analogue and Prognostic Prediction of Their Clinical Outcomes

10.21203/rs.3.rs-690208/v1 ◽

2021 ◽

Author(s):

Masato Katayama ◽

Yukina Uemura

Keyword(s):

Serum Amyloid A ◽

Total Bilirubin ◽

Clinical Signs ◽

Serum Amyloid ◽

Recurrent Fever ◽

Viral Gene ◽

Viral Agent ◽

Feline Infectious Peritonitis ◽

Amyloid A ◽

G Ratio

Abstract Background: Feline infectious peritonitis (FIP) is a fatal disease caused by feline coronavirus or its mutated pathogen designated as FIP virus. The most common form of FIP is wet or effusive, with non- regenerative anemia and clinical signs of mainly non-specific, such as recurrent fever, anorexia and weight loss. Recently, promising results using new anti-viral drug for treating cats with FIP were observed, but identification of rescuable FIP has been still challenging. It is highly worth to identify infected cats possible to be saved by such an anti-viral agent.Methods: At the initial veterinarian’s examination, owner inquiry-based signalments, viral gene detection by PCR and representative laboratory tests for diagnosis of FIP including hematocrit, A to G ratio, total bilirubin, serum amyloid-A and α1-acid globulin of 141 cats with effusive FIP were compared with those of 28 non-FIP disease cats. Consequently, 116 of them were rescued by administration of anti-viral drug Mutian X and the residual 25 were deceased unfortunately under treatments. Clinical and laboratory indicators observed prior to initial medication were also evaluated statistically between survived and non-survived groups.Results: Expectedly, levels for a few items of signalments (appetitive and activity scores), hematocrit, A to G ratio, total bilirubin, serum amyloid-A, α1-acid globulin and viral gene were found to be distributed distinctively between 141 FIP and 28 non-FIP cats. In the comparison between survived and non-survived FIP cats, most of their parameters including levels for hematocrit, A to G ratio, serum amyloid-A, α1-acid globulin and viral gene were not statistically different. Interestingly, total bilirubin concentrations of survived FIP cats were declined significantly than those of non-survived, and similarly, body temperatures, appetitive and activity scores appeared to be higher probably in accordance with their physical condition.Conclusions: Several clinical and laboratory indicators were informative in diagnosis of effusive FIP. We have investigated that one of the quantitative markers, total bilirubin levels, tend to be distributed characteristically in rescuable cats with effusive FIP. Elevated levels of total bilirubin may be a prognostic risk factor for severe FIP, predicting no clinical benefit obtained by using Mutian X as a therapeutic agent.

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Distribution of serum amyloid A and establishment of reference intervals in healthy adults

Journal of Clinical Laboratory Analysis ◽

10.1002/jcla.23120 ◽

2019 ◽

Vol 34 (4) ◽

Author(s):

Qian Liu ◽

Yan Li ◽

Fumeng Yang ◽

Tongdao Xu ◽

Li Yao ◽

...

Keyword(s):

Serum Amyloid A ◽

Reference Intervals ◽

Healthy Adults ◽

Serum Amyloid ◽

Amyloid A

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Determination of selected acute phase proteins during the treatment of limb diseases in dairy cows

Veterinární Medicína ◽

10.17221/1920-vetmed ◽

2008 ◽

Vol 53 (No. 4) ◽

pp. 173-183 ◽

Cited By ~ 15

Author(s):

P. Jawor ◽

S. Steiner ◽

T. Stefaniak ◽

W. Baumgartner ◽

A. Rzasa

Keyword(s):

Treatment Group ◽

Dairy Cows ◽

Acute Phase ◽

Serum Amyloid A ◽

Acute Phase Proteins ◽

Acute Phase Protein ◽

Serum Amyloid ◽

Blood Samples ◽

Amyloid A ◽

Phase Protein

The purpose of this study was to assess the diagnostic value of fibrinogen, haptoglobin, and serum amyloid A determination in the monitoring of the treatment of limb diseases in dairy cows. Fourteen lame cows were examined, while 10 clinically healthy cows constituted the control group. Blood samples from the ill animals were collected on three occasions: (1) upon arrival at the clinic, (2) between the third and sixth day after arriving, and (3) upon return to the owner. Blood samples from the control cows were collected once. Plasma levels of fibrinogen, haptoglobin, serum amyloid A, and total serum protein and its fractions (albumin, α-, β-, γ-globulins) were measured. Significantly higher fibrinogen, haptoglobin, and serum amyloid A levels were observed in the affected cows upon arrival at the clinic than in the control cows. Based on the changes in fibrinogen, haptoglobin, and serum amyloid A concentrations, the cows were divided into those with a systematic decrease in acute-phase protein levels during treatment (Group I, n = 6) and those which showed an increase in one or more acute-phase proteins despite treatment (Group II, n = 8). A stepwise decrease in the examined acute-phase proteins was observed in the first group and indicated an uncomplicated course of treatment; however, treatment of the second group did not appear to be wholly successful. A majority of the cows under treatment (n = 13) exhibited abnormal levels of the examined acute-phase proteins upon return to the owner. This indicates that these patients did not recover completely. The monitoring of plasma acute-phase protein concentrations can be a valuable complement to the clinical assessment of the treatment course and in the early detection of disease complications.

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Effect of adaptation stress on blood indices of limousine cows

Biotechnology in Animal Husbandry ◽

10.2298/bah0702009r ◽

2007 ◽

Vol 23 (5-6-2) ◽

pp. 9-15

Author(s):

A. Rząsa ◽

P. Nowakowski ◽

A. Dobicki ◽

R. Kwaśnicki ◽

R. Mordak

Keyword(s):

Serum Amyloid A ◽

Beef Cows ◽

Globulin Fraction ◽

Blood Samples ◽

Blood Indices ◽

Group Iii ◽

Amyloid A ◽

Elapsed Time ◽

Group I ◽

Whole Blood Samples

The aim of research was to assess glucose (Glu), fibrinogen (Fb) and hematocrit (Ht) in the whole blood samples, while cortisol (Cort), haptoglobin (Hp), serum amyloid A (SAA), total protein (TPt) and concentrations of protein fractions (albumins, globulins) in blood serum to monitor adaptation stress in beef cows of Limousine breed. Three groups of cows, each consisting of 8 animals shortly after parturition, were studied simultaneously after different time of adaptation to the new herd (Group I - 1 week, Group II - 3 weeks, Group III - 1 year). Group III animals were perceived as already adapted to the herd environment. Limousine cows with the shortest time of stay in the new herd characterized with elevated concentrations of Glu (P<0.01), Cort, SAA and ?-globulin fraction while concentration of ?-globulins was significantly lowered (P?0.05). These indices may be related to adaptation stress lasting ca 1 week. The most sensitive response of animals to adaptation stress lasting 1 week was exhibited in elevated Glu values. Cows with 3 weeks elapsed time from relocation exhibited only elevated Ht and SAA values.

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