Deficiency of vitamin D receptor in keratinocytes augments dermal fibrosis and inflammation in a mouse model of HOCl-induced scleroderma

2021 ◽  
Author(s):  
Yicheng Ge ◽  
Jing Luo ◽  
Dan Li ◽  
Chenxi Li ◽  
Junkai Huang ◽  
...  
Keyword(s):  
Vitamin D ◽  
Mouse Model ◽  
Vitamin D Receptor ◽  
Dermal Fibrosis

scholarly journals Impact of Vitamin D Receptor‐Mediated Signaling and Epithelial‐Stromal Interactions on Tumor Progression in the LPB‐Tag Mouse Model of Prostate Cancer

2009 ◽  
Vol 23 (S1) ◽  
Author(s):  
Sarah Elizabeth Mordan‐McCombs ◽  
Ann‐Christin Gaupel ◽  
JoEllen Welsh ◽  
Martin Tenniswood
Keyword(s):  
Prostate Cancer ◽  
Vitamin D ◽  
Mouse Model ◽  
Tumor Progression ◽  
Vitamin D Receptor

scholarly journals The selective vitamin D receptor agonist, elocalcitol, reduces endometriosis development in a mouse model by inhibiting peritoneal inflammation

2012 ◽  
Vol 27 (7) ◽  
pp. 2010-2019 ◽  
Author(s):  
M. Mariani ◽  
P. Vigano ◽  
D. Gentilini ◽  
B. Camisa ◽  
E. Caporizzo ◽  
...  
Keyword(s):  
Vitamin D ◽  
Mouse Model ◽  
Vitamin D Receptor ◽  
Receptor Agonist ◽  
Peritoneal Inflammation

scholarly journals Presence of a Truncated Form of the Vitamin D Receptor (VDR) in a Strain of VDR-Knockout Mice

Endocrinology ◽  
2005 ◽  
Vol 146 (12) ◽  
pp. 5581-5586 ◽  
Author(s):  
Craig M. Bula ◽  
Johanna Huhtakangas ◽  
Christopher Olivera ◽  
June E. Bishop ◽  
Anthony W. Norman ◽  
...  
Keyword(s):  
Vitamin D ◽  
Mouse Model ◽  
Vitamin D Receptor ◽  
Dissociation Constants ◽  
Specific Binding ◽  
Pcr Analysis ◽  
Wild Type ◽  
Truncated Form ◽  
Vdr Gene ◽  
Exon 2

As part of our studies on the membrane-initiated actions of 1α,25-dihydroxyvitamin D3 [1α,25(OH)2D3] and its localization in caveolae membrane fractions, we used a vitamin D receptor (VDR)-knockout (KO) mouse model to study the binding of [3H]-1α,25(OH)2D3 in the presumed absence of the VDR. In this mouse model, known as the Tokyo strain, the second exon of the VDR gene, which encodes the first of the two zinc fingers responsible for DNA binding, was removed, and the resulting animals have been considered to be VDR-null mice. To our surprise, several tissues in these KO mice showed significant (5–50% of that seen in wild-type animals) specific binding of [3H]-1α,25(OH)2D3 in nuclear and caveolae membrane fractions. The dissociation constants of this binding in samples from VDR-KO and wild-type mice were indistinguishable. RT-PCR analysis of intestinal mRNA from the VDR-KO animals revealed an mRNA that lacks exon 2 but contains exons 3–9 plus two 5′-untranslated exons. Western analysis of intestinal extracts from VDR-KO mice showed a protein of a size consistent with the use of Met52 as the translational start site. Transfection of a plasmid construct containing the sequence encoding the human analog of this truncated form of the receptor, VDR(52-C), into Cos-1 cells showed that this truncated form of the receptor retains full [3H]-1α,25(OH)2D3 binding ability. This same construct was inactive in transactivation assays using the osteocalcin promoter in CV1 cells. Thus, we have determined that this widely used strain of the VDR-KO mouse can express a form of the VDR that can bind ligand but not activate gene transcription.

scholarly journals Artesunate interacts with the vitamin D receptor to reverse sepsis‐induced immunosuppression in a mouse model via enhancing autophagy

2020 ◽  
Vol 177 (18) ◽  
pp. 4147-4165 ◽  
Author(s):  
Shenglan Shang ◽  
Jiaqi Wu ◽  
Xiaoli Li ◽  
Xin Liu ◽  
Pan Li ◽  
...  
Keyword(s):  
Vitamin D ◽  
Mouse Model ◽  
Vitamin D Receptor

scholarly journals EB1089, a vitamin D receptor agonist, reduces proliferation and decreases tumor growth rate in a mouse model of hormone-induced mammary cancer

2005 ◽  
Vol 229 (2) ◽  
pp. 205-215 ◽  
Author(s):  
Erin L. Milliken ◽  
Xiaoxue Zhang ◽  
Chris Flask ◽  
Jeffrey L. Duerk ◽  
Paul N. MacDonald ◽  
...  
Keyword(s):  
Vitamin D ◽  
Growth Rate ◽  
Mouse Model ◽  
Tumor Growth ◽  
Vitamin D Receptor ◽  
Receptor Agonist ◽  
Mammary Cancer ◽  
Tumor Growth Rate

scholarly journals Brain Endothelial P-Glycoprotein Level Is Reduced in Parkinson’s Disease via a Vitamin D Receptor-Dependent Pathway

2020 ◽  
Vol 21 (22) ◽  
pp. 8538
Author(s):  
Hyojung Kim ◽  
Jeong-Yong Shin ◽  
Yun-Song Lee ◽  
Seung Pil Yun ◽  
Han-Joo Maeng ◽  
...  
Keyword(s):  
Parkinson’S Disease ◽  
Vitamin D ◽  
Parkinson's Disease ◽  
Endothelial Cells ◽  
Mouse Model ◽  
Vitamin D Receptor ◽  
Target Genes ◽  
Umbilical Vein ◽  
Dopamine Neurons ◽  
P Glycoprotein

The progressive neurodegeneration in Parkinson’s disease (PD) is accompanied by neuroinflammation and endothelial vascular impairment. Although the vitamin D receptor (VDR) is expressed in both dopamine neurons and brain endothelial cells, its role in the regulation of endothelial biology has not been explored in the context of PD. In a 6-hydroxydopamine (6-OHDA)-induced PD mouse model, we observed reduced transcription of the VDR and its downstream target genes, CYP24 and MDR1a. The 6-OHDA-induced transcriptional repression of these genes were recovered after the VDR ligand—1α,25-dihydroxyvitamin D3 (1,25(OH)2D3) treatment. Similarly, reduced vascular protein expression of P-glycoprotein (P-gp), encoded by MDR1a, after 6-OHDA administration was reversed by 1,25(OH)2D3. Moreover, marked reduction of endothelial P-gp expression with concomitant α-synuclein aggregation was found in a combinatorial AAV-αSyn/αSyn preformed fibril (PFF) injection mouse model and postmortem PD brains. Supporting the direct effect of α-synuclein aggregation on endothelial biology, PFF treatment of human umbilical vein endothelial cells (HUVECs) was sufficient to induce α-synuclein aggregation and repress transcription of the VDR. PFF-induced P-gp downregulation and impaired functional activity in HUVECs completely recovered after 1,25(OH)2D3 treatment. Taken together, our results suggest that a dysfunctional VDR-P-gp pathway could be a potential target for the maintenance of vascular homeostasis in PD pathological conditions.

Skeletal characterization of an osteoblast-specific vitamin D receptor transgenic (ObVDR-B6) mouse model

2016 ◽  
Vol 164 ◽  
pp. 331-336 ◽  
Author(s):  
Rahma Triliana ◽  
Nga N. Lam ◽  
Rebecca K. Sawyer ◽  
Gerald J. Atkins ◽  
Howard A. Morris ◽  
...  
Keyword(s):  
Vitamin D ◽  
Mouse Model ◽  
Vitamin D Receptor
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