Effect of cereal extracts and cereal fiber on viability of Lactobacillus plantarum under gastrointestinal tract conditions

2006 ◽  
Vol 28 (1) ◽  
pp. 73-78 ◽  
Author(s):  
Haruhito Michida ◽  
Sriappareddy Tamalampudi ◽  
Severino S. Pandiella ◽  
Colin Webb ◽  
Hideki Fukuda ◽  
...  
Keyword(s):  
Gastrointestinal Tract ◽  
Lactobacillus Plantarum ◽  
Cereal Fiber

Viability of microencapsulated Akkermansia muciniphila and Lactobacillus plantarum during freeze-drying, storage and in vitro simulated upper gastrointestinal tract passage

2018 ◽  
Vol 9 (11) ◽  
pp. 5868-5879 ◽  
Author(s):  
Martín Sebastián Marcial-Coba ◽  
Tomasz Cieplak ◽  
Thiago Barbosa Cahú ◽  
Andreas Blennow ◽  
Susanne Knøchel ◽  
...  
Keyword(s):  
Gastrointestinal Tract ◽  
Lactobacillus Plantarum ◽  
Freeze Drying ◽  
Storage Stability ◽  
Upper Gastrointestinal Tract ◽  
Akkermansia Muciniphila ◽  
Freeze Dried ◽  
Upper Gastrointestinal

Microencapsulated and subsequently freeze-dried cells showed acceptable storage stability and enhanced survival during in vitro upper gastrointestinal tract passage.

scholarly journals Survival and persistence of Lactobacillus plantarum 4.1 and Lactobacillus reuteri 3S7 in the gastrointestinal tract of pigs

2007 ◽  
Vol 123 (1-3) ◽  
pp. 133-144 ◽  
Author(s):  
Maria De Angelis ◽  
Sonya Siragusa ◽  
Leonardo Caputo ◽  
Adriano Ragni ◽  
Roberto Burzigotti ◽  
...  
Keyword(s):  
Gastrointestinal Tract ◽  
Lactobacillus Plantarum ◽  
Lactobacillus Reuteri

scholarly journals Genetic Characterization of the Bile Salt Response in Lactobacillus plantarum and Analysis of Responsive Promoters In Vitro and In Situ in the Gastrointestinal Tract

2004 ◽  
Vol 186 (23) ◽  
pp. 7829-7835 ◽  
Author(s):  
Peter A. Bron ◽  
Maria Marco ◽  
Sally M. Hoffer ◽  
Esther Van Mullekom ◽  
Willem M. de Vos ◽  
...  
Keyword(s):  
Gastrointestinal Tract ◽  
Lactobacillus Plantarum ◽  
Reverse Transcription Pcr ◽  
Genome Wide ◽  
Associated Functions ◽  
Maximal Growth Rate ◽  
Genetic Responses ◽  
In Cells

ABSTRACT In this paper we describe the growth, morphological, and genetic responses of Lactobacillus plantarum WCFS1 to bile. Growth experiments revealed that a stepwise increase in the porcine bile concentration led to a gradual decrease in the maximal growth rate. Moreover, the final density reached by an L. plantarum culture growing in MRS containing 0.1% bile was approximately threefold lower than that in MRS lacking bile. The morphology of the cells grown in MRS containing 0.1% bile was investigated by scanning electron microscopy, which revealed that cells clumped together and had rough surfaces and that some of the cells had a shrunken and empty appearance, which clearly contrasted with the characteristic rod-shaped, smooth-surface morphology of L. plantarum cells grown in MRS without bile. An alr complementation-based genome-wide promoter screening analysis was performed with L. plantarum, which led to identification of 31 genes whose expression was potentially induced by 0.1% porcine bile. Remarkably, 11 membrane- and cell wall-associated functions appeared to be induced by bile, as were five functions involved in redox reactions and five regulatory factors. Moreover, the lp_0237 and lp_0775 genes, identified here as genes that are inducible by bile in vitro, were previously identified in our laboratory as important for L. plantarum in vivo during passage in the mouse gastrointestinal tract (P. A. Bron, C. Grangette, A. Mercenier, W. M. de Vos, and M. Kleerebezem, J. Bacteriol. 186:5721-5729, 2004). A quantitative reverse transcription-PCR approach focusing on these two genes confirmed that the expression level of lp_0237 and lp_0775 was significantly higher in cells grown in the presence of bile and cells isolated from the mouse duodenum than in cells grown on laboratory medium without bile.

scholarly journals Potential Thermotolerant Lactobacilli Isolated from Chicken Gastrointestinal Tract for Probiotic Use in Poultry Feeds

2020 ◽  
Vol 36 (2) ◽  
pp. 63-68
Author(s):  
M Mizanur Rahaman ◽  
M Nur Hossain ◽  
Nantu Chandra Das ◽  
Monzur Morshed Ahmed ◽  
M Mahfuzul Hoque
Keyword(s):  
Gastrointestinal Tract ◽  
Salt Tolerance ◽  
Lactobacillus Plantarum ◽  
Lactobacillus Acidophilus ◽  
Poultry Feed ◽  
Penicillin G ◽  
Lactobacillus Species ◽  
Klebsiella Pneumonia ◽  
Chloride Salt ◽  
Potential Candidate

This research was carried out to isolate and identify thermotolerant lactobacilli from broiler chicken gastrointestinal tract from Dhaka, Bangladesh. Twenty five thermotolerant Lactobacillus strains were isolated and identified as Lactobacillus species based on cultural characteristics, biochemical tests, and sugar fermentation. The probiotic are a live microbial feed supplements which positively affects the health of the host animal by improving its intestinal balance.The results showed only four among twenty five isolates were highly responded to probiotic criteria such as low pH tolerance (2.5), bile salt tolerance (2%) and sodium chloride salt tolerance (5%) in vitro, and were identified strains as Lactobacillus plantarum img-02, Lactobacillus plantarum img-08, Lactobacillus plantarum img-10 and Lactobacillus acidophilus img-14 according to Bergey’s Manual of Systematic Bacteriology. Four isolates grow enough at 45°C and two isolates (Lactobacillus plantarum img-10 and Lactobacillus acidophilus img-14) heat stable at 60°C for 30 min. All the strains were showed protease activity on skim milk agar. These strains were resistance to commercial antibiotic ciprofloxacin, gentamycin, imipenem and penicillin G but sensitive to ampicillin, doxycycline, erythromycin and tetracycline; also have antimicrobial activity against common pathogen such as ATCC of Salmonella enteritidis, Staphylococcus aureus, Listeria monocytogenes, Shigella flexneri, Bacillus cereus, Klebsiella pneumonia, Escherichia coli (environmental isolates), Aspergillus flavus and Candida albicans. This study concludes that these isolates may be used as potential candidate as probiotic poultry feed. Bangladesh J Microbiol, Volume 36 Number 2 December 2019, pp 63-68

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