scholarly journals An integrative investigation on the efficacy of Plantaginis semen based on UPLC-QTOF-MS metabolomics approach in hyperlipidemic mice

2019 ◽  
Vol 115 ◽  
pp. 108907 ◽  
Author(s):  
Xiaomeng Sun ◽  
Jiping Lan ◽  
Renchao Tong ◽  
Haoyue Zhang ◽  
Shuai Sun ◽  
...  
Keyword(s):  
Plantaginis Semen

Lipidomics Study of the Therapeutic Mechanism of Plantaginis Semen in Potassium Oxonate-Induced Hyperuricemia Rat

2020 ◽  
Author(s):  
Wenjun Shi ◽  
Fei Yang ◽  
Liting Wang ◽  
Nankun Qin ◽  
Chengxiang Wang ◽  
...  
Keyword(s):  
Male Rats ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
High Dose ◽  
Intragastric Administration ◽  
Group Model ◽  
Model Group ◽  
Tnf Α ◽  
Plantaginis Semen ◽  
Potassium Oxonate

Abstract BackgroundPlantaginis semen has been widely used as folk medicine and health care food against hyperuricemia (HUA) and gout, but little was known about its pharmacological mechanism. MethodsThe model was established by potassium oxonate intragastric administration. 42 Sprague-Dawley (SD) male rats were randomly divided into the control group, model group, benzbromarone group (10 mg/kg) and three Plantaginis semen groups (n = 7). The Plantaginis semen groups were treated orally with Plantaginis semen at 0.9375, 1.875 and 3.75 g/kg for 28 days. The levels of serum uric acid (UA), creatinine (Cr), triacylglycerol (TG) and tumor necrosis factor-α (TNF-α) were detected using enzyme-linked immunosorbent assay kits. Ultra performance liquid chromatography quadrupole time of flight mass spectrometry (UPLC-Q-TOF/MS) was used as the basis for serum lipidomics analysis, and orthogonal partial least squares discriminant analysis (OPLS-DA) was carried out for the pattern recognition and characteristic metabolites identification. The relative levels of critical regulatory factors of urate anion transporter 1(URAT1) and phosphatidylinositol 3-kinase/ protein kinases B (PI3K/Akt) were determined by quantitative real-time polymerase chain reaction (RT-qPCR). ResultsCompared with the model group, the levels of serum UA, Cr, and TG were significantly (p<0.01) decreased in benzbromarone and three Plantaginis semen groups and the level of serum TNF-α was significantly (p<0.05) decreased in benzbromarone and low dose of Plantaginis semen group. With lipidomics analysis, significant lipid metabolic perturbations were observed in HUA rats, 13 metabolites were identified as potential biomarkers and glycerophospholipid metabolism pathway was mostly affected. These perturbations can be partially restored via treatment of benzbromarone and Plantaginis semen. Additionally, the URAT1 and PI3K/Akt mRNA expression levels were significantly decreased (p<0.05) after treatment with benzbromarone and high dose of Plantaginis semen. ConclusionsPlantaginis semen had significant anti-HUA, anti-inflammatory and renal protection effects and could attenuate potassium oxonate-induced HUA through regulation of lipid metabolism disorder. Trial registrationNot applicable

Monographie 41: Plantaginis semen (Cheqianzi 车前子)

2018 ◽  
pp. 361-359
Author(s):  
C. Hu ◽  
R. Nögel ◽  
J. Hummelsberger ◽  
U. Engelhardt
Keyword(s):  
Plantaginis Semen

scholarly journals Search of anti-allodynic compounds from Plantaginis Semen, a crude drug ingredient of Kampo formula “Goshajinkigan”

2019 ◽  
Vol 73 (4) ◽  
pp. 761-768 ◽  
Author(s):  
Kazufumi Toume ◽  
Zhiyan Hou ◽  
Huanhuan Yu ◽  
Mitsuru Kato ◽  
Miki Maesaka ◽  
...  
Keyword(s):  
Crude Drug ◽  
Drug Ingredient ◽  
Plantaginis Semen

scholarly journals Effects of Plantaginis Semen & Poria (CJB) Water Extract on Histomorphometrical Aspects and Histopathological Changes in High Fat Diet-induced Obese Rats

2013 ◽  
Vol 28 (1) ◽  
pp. 91-96 ◽  
Author(s):  
Jeong Soo Hwang ◽  
Jang Mi Suk ◽  
Hye min Choi ◽  
Insoon Shin ◽  
Su Jung Hwang ◽  
...  
Keyword(s):  
High Fat Diet ◽  
Water Extract ◽  
Histopathological Changes ◽  
High Fat ◽  
Obese Rats ◽  
Plantaginis Semen

scholarly journals Diuretic Effect and Metabolomics Analysis of Crude and Salt-Processed Plantaginis Semen

2020 ◽  
Vol 11 ◽  
Author(s):  
Chao Li ◽  
Rou Wen ◽  
De Wen Liu ◽  
Qiang Liu ◽  
Li Ping Yan ◽  
...  
Keyword(s):  
Clinical Application ◽  
Urine Volume ◽  
Holistic Approach ◽  
Integrated Approach ◽  
Underlying Mechanism ◽  
Diuretic Effect ◽  
Health Products ◽  
Sphingosine 1 Phosphate ◽  
Ethanol Extracts ◽  
Plantaginis Semen

Plantaginis Semen (PS) is well recognized in traditional Chinese medicine (TCM) and health products. Crude PS (CPS) and salt-processed CPS (SPS) are the two most commonly used decoction pieces of PS, and are included in the 2020 edition of Chinese Pharmacopoeia. Although they all have multiple effects, the mechanisms for treating diseases are different and remain unclear, the processing mechanism of SPS is also indeterminate, which hinders their clinical application to a certain extent. In order to solve these problems and further develop PS in the clinical application. Here, we used saline-loaded model rats for experiments, and utilized an integrated approach consisting of pharmacological methods and metabolomics, which could assess the diuretic impact of CPS and SPS ethanol extracts on saline-loaded rats and elucidate the underlying mechanism. The results showed that CPS and SPS both produced increased urine volume excretion and urine electrolyte excretion, but the levels of aldosterone (ALD) and aquaporin 2 (AQP2) were decreased. And 30 differential metabolites such as linoleic acid, lysoPC(O-18:0), sphingosine-1-phosphate, lysoPC(18:0) were found, mainly involving three metabolic pathways. In conclusion, CPS and SPS both have a diuretic effect, and that of SPS is better. This work investigated the possible diuretic mechanisms of CPS and SPS which may also be the mechanism of PS for anti-hypertension. In addition, a holistic approach provided novel and helpful insights into the underlying processing mechanisms of TCM.

scholarly journals Antigout Effects of Plantago asiatica: Xanthine Oxidase Inhibitory Activities Assessed by Electrochemical Biosensing Method

2018 ◽  
Vol 2018 ◽  
pp. 1-11 ◽  
Author(s):  
Jin-Xiang Zeng ◽  
Juan Wang ◽  
Shou-Wen Zhang ◽  
Ji-Xiao Zhu ◽  
Min Li ◽  
...  
Keyword(s):  
Electron Transfer ◽  
Myristic Acid ◽  
Enzymatic Reaction ◽  
Accurate Method ◽  
Inhibitory Effects ◽  
Electrochemical Biosensing ◽  
Plantago Asiatica ◽  
Ic50 Values ◽  
Transfer Signal ◽  
Plantaginis Semen

The XOD inhibitory effects of Plantaginis Semen, that is, the seeds of P. asiatisca, and its representative four single compounds, acteoside, 1H-indolo-3-carbaldehyde, isoacteoside, and myristic acid, were evaluated by electron transfer signal blocking activities (ETSBA), which is based on the electron transfer signal of XOD enzymatic reaction. The blocking activities were detected using an electrochemical biosensing method. Compared with control, significant effects were observed after the addition of P. asiatica extract, acteoside, and 1H-indolo-3-carbaldehyde (all p<0.05). The IC50 values of the extract and acteoside are 89.14 and 7.55 μg·mL−1, respectively. The IC20 values of the extract, acteoside, and 1H-indolo-3-carbaldehyde are 24.28, 3.88, and 16.16 μg·mL−1, respectively. Due to the relatively lower inhibitory potential of 1H-indolo-3-carbaldehyde, its IC50 was not obtained. In addition, isoacteoside and myristic acid did not show any XOD inhibitory effects. Our data demonstrated that the XOD inhibitory effects of the extract, acteoside, and 1H-indolo-3-carbaldehyde can be accurately evaluated by the ETSBA method. The results from this study indicated that Plantaginis Semen significantly inhibited XOD activities to reduce hyperuricemia and treat gout. The study also proves that measuring the electron transfer signal blocking activities is a simple, sensitive, and accurate method to evaluate the XOD inhibitory effects.

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