Effects of spawn, supplement and phase II compost additions and time of re-casing second break compost on mushroom (Agaricus bisporus) yield and biological efficiency

2009 ◽  
Vol 100 (21) ◽  
pp. 5277-5282 ◽  
Author(s):  
Daniel J. Royse ◽  
William Chalupa
2021 ◽  
Author(s):  
Maria Luisa Tello ◽  
Rebeca Lavega ◽  
Margarita Pérez ◽  
Antonio J. Pérez ◽  
Michael Thon ◽  
...  

Abstract The cultivation of edible mushroom is an emerging sector with a potential yet to be discovered. Unlike plants, it is a less developed agriculture where many studies are lacking to optimize the cultivation. Mushrooms are a source of resources still to be revealed, which have applications not only in food, but in many other sectors such as health, industry and biotechnology. Mushroom cultivation consists of the development of selective substrates through composting where the mushroom grows via solid fermentation process. In case of Agaricus bisporus, the compost fully colonized by mycelium hardly produces mushrooms and it is necessary to apply a casing layer with certain physical, chemical and biological characteristics to shift from the vegetative mycelium to the reproductive one, where the native microbiota plays crucial roles. Currently, the industry faces a challenge to substitute the actual peat based casing materials due to the limited natural resources and the impact on the peatlands where peat is extracted.In this work we have employed high-throughput techniques by next generation sequencing to screen the microbial structure of casing soil employed in mushroom cultivation while sequencing V3-V4 of the 16S rRNA gene for bacteria and the ITS2 region of rRNA for fungi in an Illumina MiSeq. In addition, the microbiome dynamics and evolution (bacterial and fungal communities) in peat based casing along the process of incubation of Agaricus bisporus have been studied, while comparing the effect of fungicidal treatment (Chlorothalonil and Metrafenone). Statistically significant changes in populations of bacteria and fungi were observed. Microbial composition differed significantly based on incubation day, changing radically from the original communities to a specific microbial composition adapted to enhance the A. bisporus mycelium growth. Chlorothalonil treatment seems to delay casing colonization by A. bisporus. Proteobacteria and Bacteroidota appeared as the most dominant bacterial phyla. We observed a great change in the structure of the bacteria populations between day 0 and the following days. Fungi populations changed more gradually, A. bisporus displacing the rest of the species as the cultivation cycle progresses. A better understanding of the microbial communities in the casing will hopefully allow us to increase the biological efficiency during production as well as possibly help us to have a clearer view of the microbial community-pathogen relationships as they are directly related to disease development.


2016 ◽  
Vol 8 (1) ◽  
pp. 454-458
Author(s):  
Rakhi Murmu ◽  
Abhilasha A. Lal

This study was carried out during 2012-2014 to determine the effect of locally available casing materials with association of bio-inoculant Pseudomonas putida. Six different combinations of casing mixtures were amended for evaluating its effect on yield, biological efficiency, protein and carbohydrate contents of Agaricus bisporus. A mixture of FYM + GLS + Vermi-compost + P. putida gave higher mushroom yield, biological efficiency, protein and carbohydrate content. It gave better yield (1306 g), biological efficiency (28.7%), protein (34.07%) and carbohydrate content (5.07%) respectively when compared with other treatments. In addition, waste tea leaves took minimum period (33.00 days) for initiation of pin head when compared with others. Locally available casing materials along with P. putida incorporated in the casing soil can be an important factor to obtain maximum and assured yield in mushroom cultivation.


2021 ◽  
Vol 12 (5) ◽  
pp. 532-540
Author(s):  
Manisha Saini ◽  
◽  
R. S. Jarial ◽  
Kumud Jarial ◽  
◽  
...  

Studies were conducted to evaluate leached sawdust as a casing substrate for the cultivation of Agaricus bisporus either alone or in combination with other substrates at College of Horticulture and Forestry, Neri, Hamirpur, Himachal Pradesh, India during the year 2018–2020. Out of seven casing media evaluated alone, coconut coir pith performed best in terms of all production parameters like time for spawn run (20.3 days) and pinning (15.7 days), sporocarps’ number (55.33), yield (0.679 kg 5 kg-1 compost) and biological efficiency (33.95%). The efficiency of sawdust- leached improved with the addition of other casing substrates. In commercial trial, combination of sawdust - leached with coconut coir pith, spent compost and farmyard manure (1:1v/v) performed better as compared to control (FYM+local soil), but a combination of sawdust- leached+coconut coir pith (1:1v/v) proved to be the best in terms of sporocarps’ number (88.00) and yield (1.271 kg 10 kg-1 compost) despite of the delay in time for spawn run (26 days) and pinning (24.3 days). Casing media also affected the biological efficiency of compost which varied from 10.50% in local soil to 34.05% in sawdust- leached+farmyard manure (1:1v/v) in different experiments. Cap diameter was recorded to be maximum (41.37 mm) in 2 years old spent compost and minimum (34. 27 mm) in sawdust- leached while, stipe length ranged from 14.17 mm in vermicompost-leached to 22.90 mm in sawdust- leached+farmyard manure (1:1v/v). However, fruit bodies harvested from all the casing treatments including standard check were found to be silky smooth and firm to compact.


1995 ◽  
Vol 73 (S1) ◽  
pp. 1019-1024 ◽  
Author(s):  
Gerben Straatsma ◽  
Tineke W. Olijnsma ◽  
Jan P. G. Gerrits ◽  
Leo J. L. D. Van Griensven ◽  
Robert A. Samson ◽  
...  

Agaricus bisporus mushrooms are cultivated on straw compost prepared in the sequence phase I and phase II. Phase I is a process at 70–80 °C for several days up to 2 weeks. Phase II is a controlled process at 45 °C for 6 days. In phase II the thermophilic fungus Scytalidium thermophilum becomes abundant. Its density in finished compost is correlated with the yield of mushrooms. It stimulates the growth rate of mushroom mycelium. Scytalidium thermophilum was inoculated in batches of 1 tonne of pasteurized compost for experimental phase II. Mushroom yields on these composts were twice those of controls (pasteurized, not inoculated). This indicated that S. thermophilum is important for the conversion of straw to compost. The essential actions for mushroom crop productivity of high temperatures during phase I and of S. thermophilum during phase II are unclear. Key words: Agaricus bisporus, button mushroom, Scytalidium thermophilum, solid-state fermentation, processing, review.


HortScience ◽  
2010 ◽  
Vol 45 (11) ◽  
pp. 1649-1653 ◽  
Author(s):  
Diego Cunha Zied ◽  
Marli Teixeira Almeida Minhoni ◽  
J. Emilio Pardo-González ◽  
Arturo Pardo-Giménez

The aim of this study was to assess the effect of the addition of different rates of colonized compost and formaldehyde treatment on production parameters (yield, biological efficiency, mushroom number, mushroom weight, and earliness) in a Dutch commercial casing using Phase III bulk compost as a base substrate for growing Agaricus bisporus. Four rates of spawn-run compost (0 g·m−2, 200 g·m−2, 400 g·m−2, and 600 g·m−2) and formaldehyde treatment (with or without) were used. The experiment was designed as a double factorial in a randomized complete block design. Each block contained six replicates per treatment (a compost bag with 20 kg). The compost added to casing (CACing) technique when applied properly and combined with the use of Phase III compost had significant benefits, including increased mushroom yield, weight, and reduced the growing cycle, especially with the rate of 600 g of spawn-run compost/m2 of casing. On the other hand, the application of formalin reduced yield with or without the CACing technique, which implies the need for an alternative treatment for disinfection. An alternative method may either be thermal or chemical treatments to the casing such as using formaldehyde before mixing the casing with spawn-run compost and before the addition of casing above the colonized compost.


Author(s):  
K. S. McCarty ◽  
R. F. Weave ◽  
L. Kemper ◽  
F. S. Vogel

During the prodromal stages of sporulation in the Basidiomycete, Agaricus bisporus, mitochondria accumulate in the basidial cells, zygotes, in the gill tissues prior to entry of these mitochondria, together with two haploid nuclei and cytoplasmic ribosomes, into the exospores. The mitochondria contain prominent loci of DNA [Fig. 1]. A modified Kleinschmidt spread technique1 has been used to evaluate the DNA strands from purified whole mitochondria released by osmotic shock, mitochondrial DNA purified on CsCl gradients [density = 1.698 gms/cc], and DNA purified on ethidium bromide CsCl gradients. The DNA appeared as linear strands up to 25 u in length and circular forms 2.2-5.2 u in circumference. In specimens prepared by osmotic shock, many strands of DNA are apparently attached to membrane fragments [Fig. 2]. When mitochondria were ruptured in hypotonic sucrose and then fixed in glutaraldehyde, the ribosomes were released for electron microscopic examination.


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