Rational design of a culture medium for the intensification of lipid storage in Chlorella sp. Performance evaluation in air-lift bioreactor

2014 ◽  
Vol 158 ◽  
pp. 269-277 ◽  
Author(s):  
Pablo C. Giordano ◽  
Alejandro J. Beccaria ◽  
Héctor C. Goicoechea
Keyword(s):  
Performance Evaluation ◽  
Rational Design ◽  
Culture Medium ◽  
Lipid Storage ◽  
Chlorella Sp ◽  
Air Lift

Performance evaluation of different cationic flocculants through pH modulation for efficient harvesting of Chlorella sp. HS2 and their impact on water reusability

2019 ◽  
Vol 136 ◽  
pp. 819-827 ◽  
Author(s):  
Manoranjan Nayak ◽  
Naim Rashid ◽  
William I. Suh ◽  
Bongsoo Lee ◽  
Yong Keun Chang
Keyword(s):  
Performance Evaluation ◽  
Chlorella Sp

scholarly journals Performance evaluation of CHO-K1 cell in culture medium supplemented with hemolymph

2005 ◽  
Vol 48 (spe) ◽  
pp. 85-95 ◽  
Author(s):  
Tássia Raffoul ◽  
Kamilla Swiech ◽  
Mabel Karina Arantes ◽  
Álvaro Paiva Braga de Sousa ◽  
Ronaldo Zucatelli Mendonça ◽  
...  
Keyword(s):  
Performance Evaluation ◽  
Culture Medium ◽  
Cell Concentration ◽  
Animal Cell ◽  
Positive Influence ◽  
Medium Supplement ◽  
Co2 Environment

The aim of this work was to evaluate the potential of hemolymph utilization as a culture medium supplement to cultivate the animal cell CHO-K1. For this purpose 1% v/v of hemolymph was added to DMEM medium containing 10% v/v of FBS and 1 or 4.5 g/L of glucose. The culture was grown in spinner flasks incubated in a 10% v/v CO2 environment, at 37ºC, with the Cytodex 1 microcarrier. Comparing the results obtained from the culture with hemolymph against those without hemolymph, a positive influence of the hemolymph was observed, as the experiment with hemolymph presented a 52% higher cell concentration and a higher productivity of up to 40%.

scholarly journals Productivity and quality of Moina sp. cultivated with various culture medium

2021 ◽  
Vol 20 (2) ◽  
pp. 148-162
Author(s):  
Taufik Shidik Adi Nugroho Shidik ◽  
Julie Ekasari ◽  
Dedi Jusadi ◽  
Mia Setiawati
Keyword(s):  
Fatty Acids ◽  
Organic Material ◽  
Culture Medium ◽  
Unsaturated Fatty Acids ◽  
Protein Quality ◽  
Culture Media ◽  
Mass Scale ◽  
Chlorella Sp ◽  
Study Results

Cultivation of Moina sp is still constrained by its quality, productivity, and sustainability. The alternative solution is the use of cultivation media materials that have high nutritional content and easily available in large quantities to support the quality and productivity of Moina sp. and meet the needs of live feed. The objective of the study was to evaluate the effect of various culture medium on the productivity and nutritional quality of Moina sp.. Five culture media were tested in laboratory scale, i.e. organic ingredient (BO), Chlorella sp. (Ch), Chlorella sp. + organic ingredients (ChBO), biofloc (BF) and biofloc + organic ingredients (BFBO). While in mass scale, four culture media were tested, i.e. Chlorella sp. (Ch), Chlorella sp. + Organic Ingredients (ChBO), Biofloc (BF) and Biofloc + Organic Ingredients (BFBO). The peaks of Moina sp. density in different treatments were achieved in different days. ChBO treatments significantly had higher productivity (P<0.05). The highest protein content was found in Moina sp. cultured with ChBO media, even higher than artemia. Moina sp. cultured with Chlorella sp. (Ch) showed the highest PUFA (Poly Unsaturated Fatty Acids) contents, while the highest MUFA (mono unsaturated fatty acids) contents was obtained from Moina sp. cultured with BFBO media lower than artemia. The study results indicates that different culture media produces different productivity and nutrient quality of Moina sp. The organic material combination of Chlorella sp. + organic material (ChBO) was the best media to improve the productivity and protein quality of Moina sp.    Keywords : Biofloc, Chlorella sp., Moina sp., organic matter, productivity, quality   ABSTRAK   Budidaya Moina sp. masih terkendala pada kualitas, produktivitas dan kestabilan dalam ketersediaannya. Untuk itu diperlukan penggunaan bahan media budidaya yang memiliki kandungan nutrisi tinggi dan mudah didapat dalam jumlah banyak untuk mendukung kualitas dan produktivitas Moina sp. demi memenuhi kebutuhan pakan hidup. Tujuan penelitian yaitu mengevaluasi pengaruh berbagai media budidaya terhadap produktivitas dan kualitas nutrisi Moina sp. Lima media kultur yang diuji dalam penelitian laboratorium yaitu Bahan Organik (BO), Chlorella sp. (Ch), Chlorella sp. + Bahan Organik (ChBO), Bioflok (BF) dan Bioflok + Bahan Organik (BFBO). Sedangkan pada penelitian skala massal diuji empat media kultur yaitu Chlorella sp. (Ch), Chlorella sp. + Bahan Organik (ChBO), Bioflok (BF) dan Bioflok + Bahan Organik (BFBO). Puncak kepadatan Moina sp. pada tiap perlakuan dicapai pada hari yang berbeda. Perlakuan ChBO memiliki produktivitas yang lebih tinggi (P<0,05). Kandungan protein Moina sp. tertinggi ditemukan pada media ChBO dan bahkan lebih tinggi dari pada artemia. Moina sp. yang dibudidayakan dengan Chlorella sp. (Ch), menunjukkan kandungan PUFA tertinggi, sedangkan kandungan MUFA yang tertinggi terdapat pada Moina sp. yang dibudidayakan dengan bahan media BFBO namun masih lebih rendah jika dibandingkan dengan kandungan pada artemia. Hasil penelitian menunjukkan media kultur yang berbeda menghasilkan produktivitas dan kualitas nutrisi moina yang berbeda. Kombinasi bahan organik Chlorella + bahan organik (ChBO) merupakan media terbaik dibandingkan dengan perlakuan lainnya untuk meningkatkan produktivitas dan kualitas nutrisi terutama protein Moina sp.   Kata kunci : Bioflok, Chlorella sp., Moina sp., bahan organik, produktivitas, kualitas

scholarly journals A metabolic network-based approach for developing feeding strategies for CHO cells to increase monoclonal antibody production

2019 ◽  
Author(s):  
Hamideh Fouladiha ◽  
Sayed-Amir Marashi ◽  
Fatemeh Torkashvand ◽  
Fereidoun Mahboudi ◽  
Nathan E. Lewis ◽  
...  
Keyword(s):  
Cell Culture ◽  
Monoclonal Antibody ◽  
Metabolic Network ◽  
Cho Cells ◽  
Rational Design ◽  
Culture Medium ◽  
Feeding Strategies ◽  
Cell Culture Medium ◽  
Bioprocess Optimization ◽  
A Genome

AbstractChinese hamster ovary (CHO) cells are the main workhorse in the biopharmaceutical industry for the production of recombinant proteins, such as monoclonal antibodies. To date, a variety of metabolic engineering approaches have been used to improve the productivity of CHO cells. While genetic manipulations are potentially laborious in mammalian cells, rational design of CHO cell culture medium or efficient fed-batch strategies are more popular approaches for bioprocess optimization. In this study, a genome-scale metabolic network model of CHO cells was used to design feeding strategies for CHO cells to improve monoclonal antibody (mAb) production. A number of metabolites, including threonine and arachidonate, were suggested by the model to be added into cell culture medium. The designed composition has been experimentally validated, and then optimized, using design of experiment methods. About a two-fold increase in the total mAb expression has been observed using this strategy. Our approach can be used in similar bioprocess optimization problems, in order to suggest new ways of increasing production in different cell factories.

2428 Performance Evaluation and Its Prediction of Bubble-Jet-Type Air Lift Pump

2006 ◽  
Vol 2006.2 (0) ◽  
pp. 175-176
Author(s):  
Takanao KIMURA ◽  
Junichi NAKAO ◽  
Akimamo KAWAHARA ◽  
Michio SADATOMI
Keyword(s):  
Performance Evaluation ◽  
Air Lift

scholarly journals Distillery Wastewater as a Sustainable Medium for Microalgae Production

2019 ◽  
Author(s):  
Viviana Quintero-Dallos ◽  
Janet Bibiana García-Martínez ◽  
Jefferson Eduardo Contreras-Ropero ◽  
Crisostomo Barajas-Ferrerira ◽  
Andrés Fernando Barajas-Solano ◽  
...  
Keyword(s):  
Chlorella Vulgaris ◽  
Rapid Growth ◽  
Continuous Flow ◽  
Culture Medium ◽  
Biomass Productivity ◽  
Average Value ◽  
Average Production ◽  
Distillery Wastewater ◽  
Heterotrophic Metabolism ◽  
Air Lift

This study investigates distillery wastewater, commonly known as vinasse, as a potential culture medium for the production of Chlorella vulgaris and its most relevant metabolites. The effect of vinasse concentration on the composition of the biomass (proteins, carbohydrates and lipids) was evaluated in treatments performed in 6-L tubular air-lift reactors. The reactors were operated at 25 °C for 18 days, in total darkness, under a continuous flow of air. Results showed a rapid growth of microalgae in the first ten days, when an average production of 0.87 g/L was reached. Then, the daily biomass productivity began to decrease, up to an average value of 11.8 g/L at the 16th day. For all treatments, there was a significant reduction in the concentration of most metabolites in the first eight days. This was likely due to the adaptation of the biomass to the new conditions, with a the transition from autotrophic to heterotrophic metabolism. From the 10th day, the concentration of metabolites in the biomass began to increase, reaching a nearly constant value at the 16th day. The observed maximum concentrations (%w/w) were: 48.95% proteins, 2.88% xylose, 7.82% glucose, 4.54% arabinose, 8.28% fructose and 4.82% lipids. These values were only marginally affected by the type of treatment. Overall, the results obtained suggest that vinasse is a promising and sustainable medium for the growth of C. vulgaris and the production of valuable metabolites.

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