Non-ionic surfactants do not consistently improve the enzymatic hydrolysis of pure cellulose

Effects of several surfactants on the activity of acid cellulase enzyme were studied in this paper. The results show that addition of ionic surfactants to the enzyme decrease the enzymatic hydrolysis of cellulose sodium, while nonionic surfactants increase the activity of the enzyme. Among the surfactants examined, fatty amine polyoxyethylene ether (AC-1860) was the most effective. In the presence of 0.5 wt % of AC-1860, the activity of acid cellulose enzyme increased by 25.8%. This finding could lead to a reduction in the enzyme requirement for cellulose utilization.

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An Integrated Enzymatic Approach to Produce Pentyl Xylosides and Glucose/Xylose Laurate Esters From Wheat Bran

Frontiers in Bioengineering and Biotechnology ◽

10.3389/fbioe.2021.647442 ◽

2021 ◽

Vol 9 ◽

Author(s):

Chloé Jocquel ◽

Murielle Muzard ◽

Richard Plantier-Royon ◽

Caroline Rémond

Keyword(s):

Enzymatic Hydrolysis ◽

Wheat Bran ◽

Lauric Acid ◽

Integrated Approach ◽

Ionic Surfactants ◽

Alkyl Glycosides ◽

Esterification Reactions ◽

Hydrolysis Of ◽

Cellic Ctec2 ◽

Commercial Lipase

Alkyl glycosides and sugars esters are non-ionic surfactants of interest for various applications (cosmetics, food, detergency,…). In the present study, xylans and cellulose from wheat bran were enzymatically converted into pentyl xylosides and glucose and xylose laurate monoesters. Transglycosylation reaction catalyzed by the commercial enzymatic cocktail Cellic Ctec2 in the presence of pentanol led to the synthesis of pentyl β-D-xylosides from DP1 to 3 with an overall yield of 520 mg/g of xylans present in wheat bran. Enzymatic hydrolysis of wheat bran with Cellic Ctec2 and subsequent acylation of the recovered D-glucose and D-xylose catalyzed by the commercial lipase N435 in the presence of lauric acid or methyl laurate produced one D-glucose laurate monoester and one D-xylose laurate monoester. An integrated approach combining transglycosylation and (trans)esterification reactions was successfully developed to produce both pentyl xylosides and D-glucose and D-xylose laurate esters from the same batch of wheat bran.

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Non-ionic Surfactants and Non-Catalytic Protein Treatment on Enzymatic Hydrolysis of Pretreated Creeping Wild Ryegrass

Biotechnology for Fuels and Chemicals ◽

10.1007/978-1-60327-526-2_35 ◽

2007 ◽

pp. 351-368

Author(s):

Yi Zheng ◽

Zhongli Pan ◽

Ruihong Zhang ◽

Donghai Wang ◽

Bryan Jenkins

Keyword(s):

Enzymatic Hydrolysis ◽

Ionic Surfactants ◽

Hydrolysis Of ◽

Protein Treatment

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Organosolv lignin properties and their effects on enzymatic hydrolysis

BioResources ◽

10.15376/biores.15.4.8909-8924 ◽

2020 ◽

Vol 15 (4) ◽

pp. 8909-8924

Author(s):

Yang Huang ◽

Chenhuan Lai ◽

Shaolong Sun ◽

Qiang Yong ◽

Brian K. Via ◽

...

Keyword(s):

Enzymatic Hydrolysis ◽

Distribution Coefficient ◽

Chemical Properties ◽

Structural Features ◽

Hydrolysis Yield ◽

Pure Cellulose ◽

Organosolv Lignin ◽

Physico Chemical ◽

Enzyme Distribution ◽

Hydrolysis Of

Lignin plays a crucial role in enzymatic hydrolysis of lignocellulosic biomass. To evaluate the correlation between lignin properties and its effects on enzymatic hydrolysis, five organosolv lignins (OLs) were isolated from woody biomass, and their physico-chemical properties and structural features were characterized. The effects of OL addition on enzymatic hydrolysis of microcrystalline cellulose (pure cellulose) were assessed first, which showed their disparate effects. The addition of three OLs increased the 72 h hydrolysis yield by 7.4% to 10.1%, while the addition of other two OLs reduced the 72 h hydrolysis yield by 3.2% to 20.4%. A strong correlation between the enzyme distribution coefficient on lignins and the 72 h hydrolysis yields indicated that the enzyme-lignin interaction played a significant role in determining the lignin effects. More importantly, a correlation between lignin properties (hydrophobicity, zeta potential, and particle size) and the enzyme distribution coefficient was established. Identifying the key lignin properties will give insights to reduce the lignin inhibition by altering the lignin properties, thereby promoting enzymatic hydrolysis of lignocellulose.

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Comparison of the Interaction Modes Between Cellulase and Bovine Serum Albumin /Lignosulfonate /Polyethylene Glycol in Promoting Lignocellulose Saccharification

10.21203/rs.3.rs-552319/v1 ◽

2021 ◽

Author(s):

Peipei Wang ◽

Tian Liu ◽

Jiaqi Guo ◽

Yongcan Jin ◽

Huining Xiao ◽

...

Keyword(s):

Polyethylene Glycol ◽

Enzymatic Hydrolysis ◽

Bovine Serum Albumin ◽

Serum Albumin ◽

Bovine Serum ◽

Enzymatic System ◽

Promoting Effect ◽

Pure Cellulose ◽

Hydrolysis Of ◽

Nonproductive Binding

Abstract Background: Bovine serum albumin (BSA), polyethylene glycol (PEG) and lignosulfonate (LS) have been extensively employed as synergistic agents in lignocellulose saccharification, albeit it has not been fully understood how they interact with enzymes from the perspectives of molecular interactions. Herein, we attempted to unveil the promotion mechanisms of BSA, PEG and LS for lignocellulose saccharification from the perspective of their respective interaction with cellulase using Quartz Crystal Microbalance with Dissipation monitoring (QCM-D), Surface Plasmon Resonance (SPR), and Small Angle X-ray Scattering (SAXS) to investigate their respective interaction and the complex formation. In the meanwhile, we compared the effects of adding these additives into the enzymatic hydrolysis of pure cellulose (Avicel) and green liquor-pretreated lignocellulose (GL).Results: The results showed that BSA and LS could bind to cellulase to form complexes, whereas PEG did not. However, PEG had a high affinity to lignin or lignin derivatives. In term for Avicel and GL substrates, the results showed that BSA and PEG promoted the enzymatic hydrolysis of both substrates, while LS had a promoting effect for GL only and inhibited some extent for Avicel. Conclusions: This study showed that synergistic agents of LS, BSA, and PEG have different interaction modes with cellulase. BSA and LS form complexes with cellulase and the formed complexes prevent from nonproductive binding by residue lignin; whereas PEG prevents from nonproductive binding by forming a thin layer on residue lignin which actually serve as steric hindrance. This investigation will help us to understand the sophisticated interactions among the components in the complicated enzymatic system, especially the interactions between enzymes and synergistic agents. It will be helpful in the design and utilization of synergistic additives in the lignocellulose biorefinery process as well.

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Microwave-assisted enzymatic hydrolysis of starch

10.3390/ecsoc-13-00159 ◽

2009 ◽

Cited By ~ 1

Author(s):

Marcin Lukasiewicz ◽

Anna Osowiec ◽

Magdalena Marciniak

Keyword(s):

Enzymatic Hydrolysis ◽

Microwave Assisted ◽

Hydrolysis Of

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Analyses of enzymatic hydrolysis of bagasse cane of sugar for obtention of ethanol employing a cocktail of natives commercials enzymes.

10.3390/mol2net-04-05300 ◽

2018 ◽

Author(s):

Ángel Batallas ◽

Erenio González ◽

Carmen Salvador ◽

Jonathan Villavicencio ◽

Humberto González Gavilánez ◽

...

Keyword(s):

Enzymatic Hydrolysis ◽

Hydrolysis Of

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Faculty Opinions recommendation of Enzymatic hydrolysis of pneumococcal capsular polysaccharide renders the bacterium vulnerable to host defense.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.733387483.793547056 ◽

2018 ◽

Author(s):

Mattias Collin

Keyword(s):

Enzymatic Hydrolysis ◽

Host Defense ◽

Capsular Polysaccharide ◽

Hydrolysis Of

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Immobilized Nanoparticles-Mediated Enzymatic Hydrolysis of Cellulose for Clean Sugar Production: A Novel Approach

Current Nanoscience ◽

10.2174/1573413714666180611081759 ◽

2019 ◽

Vol 15 (3) ◽

pp. 296-303 ◽

Cited By ~ 5

Author(s):

Swapnil Gaikwad ◽

Avinash P. Ingle ◽

Silvio Silverio da Silva ◽

Mahendra Rai

Keyword(s):

Catalytic Activity ◽

Enzymatic Hydrolysis ◽

Immobilized Enzyme ◽

Free Enzyme ◽

Magnetic Nanomaterials ◽

Sugar Production ◽

Cellulase Enzyme ◽

Enzymatic Hydrolysis Of Cellulose ◽

Hydrolysis Of Cellulose ◽

Hydrolysis Of

Background: Enzymatic hydrolysis of cellulose is an expensive approach due to the high cost of an enzyme involved in the process. The goal of the current study was to apply magnetic nanomaterials as a support for immobilization of enzyme, which helps in the repeated use of immobilized enzyme for hydrolysis to make the process cost-effective. In addition, it will also provide stability to enzyme and increase its catalytic activity. Objective: The main aim of the present study is to immobilize cellulase enzyme on Magnetic Nanoparticles (MNPs) in order to enable the enzyme to be re-used for clean sugar production from cellulose. Methods: MNPs were synthesized using chemical precipitation methods and characterized by different techniques. Further, cellulase enzyme was immobilized on MNPs and efficacy of free and immobilized cellulase for hydrolysis of cellulose was evaluated. Results: Enzymatic hydrolysis of cellulose by immobilized enzyme showed enhanced catalytic activity after 48 hours compared to free enzyme. In first cycle of hydrolysis, immobilized enzyme hydrolyzed the cellulose and produced 19.5 ± 0.15 gm/L of glucose after 48 hours. On the contrary, free enzyme produced only 13.7 ± 0.25 gm/L of glucose in 48 hours. Immobilized enzyme maintained its stability and produced 6.15 ± 0.15 and 3.03 ± 0.25 gm/L of glucose in second and third cycle, respectively after 48 hours. Conclusion: This study will be very useful for sugar production because of enzyme binding efficiency and admirable reusability of immobilized enzyme, which leads to the significant increase in production of sugar from cellulosic materials.

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