Pyrogenic transformation of Nannochloropsis oceanica into fatty acid methyl esters without oil extraction for estimating total lipid content

2016 ◽  
Vol 212 ◽  
pp. 55-61 ◽  
Author(s):  
Jieun Kim ◽  
Jong-Min Jung ◽  
Jechan Lee ◽  
Ki-Hyun Kim ◽  
Tae O Choi ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Total Lipid ◽  
Lipid Content ◽  
Fatty Acid Methyl Esters ◽  
Methyl Esters ◽  
Total Lipid Content ◽  
Oil Extraction ◽  
Nannochloropsis Oceanica ◽  
Acid Methyl ◽  
Pyrogenic Transformation

scholarly journals Enhanced fatty acid methyl esters recovery through a simple and rapid direct transesterification of freshly harvested biomass of Chlorella vulgaris and Messastrum gracile

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Saw Hong Loh ◽  
Mee Kee Chen ◽  
Nur Syazana Fauzi ◽  
Ahmad Aziz ◽  
Thye San Cha
Keyword(s):  
Fatty Acid ◽  
Chlorella Vulgaris ◽  
Fatty Acid Methyl Esters ◽  
Methyl Esters ◽  
Oil Extraction ◽  
Direct Transesterification ◽  
Microalgae Culture ◽  
Cell Biomass ◽  
Acid Methyl ◽  
Fatty Acid Accumulation

AbstractConventional microalgae oil extraction applies physicochemical destruction of dry cell biomass prior to transesterification process to produce fatty acid methyl esters (FAMEs). This report presents a simple and rapid direct transesterification (DT) method for FAMEs production and fatty acid profiling of microalgae using freshly harvested biomass. Results revealed that the FAMEs recovered from Chlorella vulgaris were 50.1 and 68.3 mg with conventional oil-extraction-transesterification (OET) and DT method, respectively. While for Messastrum gracile, the FAMEs recovered, were 49.9 and 76.3 mg, respectively with OET and DT methods. This demonstrated that the DT method increased FAMEs recovery by 36.4% and 53.0% from C. vulgaris and M. gracile, respectively, as compared to OET method. Additionally, the DT method recovered a significantly higher amount of palmitic (C16:0) and stearic (C18:0) acids from both species, which indicated the important role of these fatty acids in the membranes of cells and organelles. The DT method performed very well using a small volume (5 mL) of fresh biomass coupled with a shorter reaction time (~ 15 min), thus making real-time monitoring of FAMEs and fatty acid accumulation in microalgae culture feasible.

scholarly journals Augmenting Fremyella diplosiphon Cellular Lipid Content and Unsaturated Fatty Acid Methyl Esters Via Sterol Desaturase Gene Overexpression

2019 ◽  
Vol 189 (4) ◽  
pp. 1127-1140 ◽  
Author(s):  
Somayeh Gharaie Fathabad ◽  
AnithaChristy S. Arumanayagam ◽  
Behnam Tabatabai ◽  
Huan Chen ◽  
Jie Lu ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Lipid Content ◽  
Unsaturated Fatty Acid ◽  
Fatty Acid Methyl Esters ◽  
Methyl Esters ◽  
Gene Overexpression ◽  
Cellular Lipid ◽  
Desaturase Gene ◽  
Fremyella Diplosiphon ◽  
Acid Methyl

scholarly journals Prospecting for Oleaginous and Robust Chlorella spp. for Coal-Fired Flue-Gas-Mediated Biodiesel Production

Energies ◽  
2018 ◽  
Vol 11 (8) ◽  
pp. 2026 ◽  
Author(s):  
Bohwa Kim ◽  
Ramasamy Praveenkumar ◽  
Eunji Choi ◽  
Kyubock Lee ◽  
Sang Jeon ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Lipid Content ◽  
Flue Gas ◽  
Fatty Acid Methyl Esters ◽  
Methyl Esters ◽  
International Standards ◽  
Biodiesel Production ◽  
Acid Methyl ◽  
G Cell ◽  
Mixotrophic Conditions

Prospecting for robust and high-productivity strains is a strategically important step in the microalgal biodiesel process. In this study, 30 local strains of Chlorella were evaluated in photobioreactors for biodiesel production using coal-fired flue-gas. Three strains (M082, M134, and KR-1) were sequentially selected based on cell growth, lipid content, and fatty acid composition under autotrophic and mixotrophic conditions. Under autotrophic conditions, M082 and M134 showed comparable lipid contents (ca. 230 mg FAME [fatty acid methyl esters derived from microalgal lipids]/g cell) and productivities (ca. 40 mg FAME/L·d) versus a reference strain (KR-1) outdoors with actual flue-gas (CO2, 13%). Interestingly, under mixotrophic conditions, M082 demonstrated, along with maximal lipid content (397 mg FAME/g cell), good tolerance to high temperature (40 °C). Furthermore, the fatty acid methyl esters met important international standards under all of the tested culture conditions. Thus, it was concluded that M082 can be a feedstock of choice for coal-fired, flue-gas-mediated biodiesel production.

scholarly journals In Situ Enzymatic Conversion of Nannochloropsis oceanica IMET1 Biomass into Fatty Acid Methyl Esters

2016 ◽  
Vol 10 (2) ◽  
pp. 438-448 ◽  
Author(s):  
Yao Wang ◽  
Yi-Ying Lee ◽  
Tonya M. Santaus ◽  
Charles E. Newcomb ◽  
Jin Liu ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Fatty Acid Methyl Esters ◽  
Methyl Esters ◽  
Enzymatic Conversion ◽  
Nannochloropsis Oceanica ◽  
Acid Methyl
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