Changes on structural properties of biomass pretreated by combined deacetylation with liquid hot water and its effect on enzymatic hydrolysis

In this work, liquid hot water pretreatment (autohydrolysis) was used to improve enzymatic hydrolysis of a commonly consumed vegetable waste in Spain, Italian green pepper, to finally produce fermentable sugars. Firstly, the effect of temperature and contact time on sugar recovery during pretreatment (in insoluble solid and liquid fraction) was studied in detail. Then, enzymatic hydrolysis using commercial cellulase was performed with the insoluble solid resulting from pretreatment. The objective was to compare results with and without pretreatment. The results showed that the pretreatment step was effective to facilitate the sugars release in enzymatic hydrolysis, increasing the global sugar yield. This was especially notable when pretreatment was carried out at 180 °C for 40 min for glucose yields. In these conditions a global glucose yield of 61.02% was obtained. In addition, very low concentrations of phenolic compounds (ranging from 69.12 to 82.24 mg/L) were found in the liquid fraction from enzymatic hydrolysis, decreasing the possibility of fermentation inhibition produced by these components. Results showed that Italian green pepper is an interesting feedstock to obtain free sugars and prevent the enormous quantity of this food waste discarded annually.

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Tissue-specific biomass recalcitrance in corn stover pretreated with liquid hot-water: Enzymatic hydrolysis (part 1)

Biotechnology and Bioengineering ◽

10.1002/bit.23337 ◽

2011 ◽

Vol 109 (2) ◽

pp. 390-397 ◽

Cited By ~ 62

Author(s):

Meijuan Zeng ◽

Eduardo Ximenes ◽

Michael R. Ladisch ◽

Nathan S. Mosier ◽

Wilfred Vermerris ◽

...

Keyword(s):

Enzymatic Hydrolysis ◽

Corn Stover ◽

Hot Water ◽

Biomass Recalcitrance ◽

Tissue Specific ◽

Liquid Hot Water ◽

Specific Biomass

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Changes in poplar (Populus trichocarpa) wood porous structure after liquid hot water (LHW) pretreatment

Annals of WULS, Forestry and Wood Technology ◽

10.5604/01.3001.0014.8861 ◽

2020 ◽

Vol 112 ◽

pp. 71-78

Author(s):

Florentyna Akus-Szyblerg ◽

Jan Szadkowski ◽

Andrzej Antczak ◽

Janusz Zawadzki

Keyword(s):

Enzymatic Hydrolysis ◽

Porous Structure ◽

Populus Trichocarpa ◽

Hot Water ◽

Size Exclusion ◽

Bioethanol Production ◽

Poplar Wood ◽

Liquid Hot Water ◽

Exclusion Chromatography ◽

Pretreatment Conditions

Changes in poplar (Populus trichocarpa) wood porous structure after liquid hot water (LHW) pretreatment. The aim of this research was to investigate the effect of applying different hydrothermal pretreatment conditions on the porous structure of poplar wood. Porosity is recognised as an important factor considering efficiency of an enzymatic hydrolysis as a step of bioethanol production. Native poplar wood as well as solid fractions after pretreatment performed at different temperatures (160 °C, 175 °C and 190 °C) were analysed. Porous structure was examined with an inverse size-exclusion chromatography (ISEC) method. Results indicated a significant development of the porous structure of the biomass with increasing porosity along with the growing temperature of the LHW process. The temperature of 190 °C was chosen as the most promising condition of poplar wood LHW pretreatment in terms of the efficiency of the subsequent steps of bioethanol production. The obtained results were consistent with the previous experimental data procured during analysis of the LHW pretreated poplar wood and its subsequent enzymatic hydrolysis yield.

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