Accelerated biomethane production from lignocellulosic biomass: Pretreated by mixed enzymes secreted by Trichoderma viride and Aspergillus sp.

Anthropogenic forcing has a huge impact on the formation of the ecosystem of modern cities, including the composition of the mycobiota of the urban environment. The paper presents the results of mycological studies of soil samples taken in the city of Kazan in areas divided into recreational and transport functional areas with different levels of anthropogenic load. More than 60 species of fungi belonging to 15 genera, including 20 species common to soils in both zones were identified. Penicillium sp., Fusarium sp., Rhizomucor sp., Trichoderma viride were shown to dominate in undisturbed natural areas, and in soils of the urban environment there was much more often found the presence of Alternaria sp., Aspergillus sp., Candida sp., Rhizopus sp., Trichophyton sp. In areas with good natural light the percentage of samples with low contamination by fungi was established to be significantly higher than in shaded areas: 16.7% and 7.1% in the recreational area; and 12.5% and 0% in the transport zone correspondingly. Potentially pathogenic species of fungi were detected in 80% of samples of recreational and in 95% of samples of transport areas. Dark-colored species (including Aspergillus, Alternaria, etc.) were shown to be more common in soils of transport zone, and Trichophyton sp. and Scopulariopsis sp. - in soils of recreational zone. The evaluation of mycological hazard index allowed to estimate the condition of the soil of Kazan as satisfactory for 80% of sites of recreational and 65% - of the transport area. The average value of the mycological hazard index for recreational areas was 2.7 and for the transport areas - 4.2. Results indicate to regular mycological monitoring would be desirable for the normalization of the mycological environment, significantly reducing the risk of mycosis, mycotoxicosis and mycogenic allergies, and should help to ensure sanitary-epidemiological welfare of the population.

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Mycoflora associated with bipolaris leaf blight of different wheat varieties in Bangladesh

Dhaka University Journal of Biological Sciences ◽

10.3329/dujbs.v28i1.46490 ◽

2019 ◽

Vol 28 (1) ◽

pp. 21-35

Author(s):

Mst Selina Momtaz ◽

Shamim Shamsi ◽

Tapan Kumar Dey

Keyword(s):

New Record ◽

Trichoderma Viride ◽

Fungal Species ◽

Leaf Blight ◽

Rhizopus Stolonifer ◽

Wheat Varieties ◽

Syncephalastrum Racemosum ◽

Aspergillus Sp ◽

Planting Method ◽

Nigrospora Oryzae

Thirty five fungal species, representing 20 genera were found to be associated with Bipolaris leaf blight infected leaves of 21 wheat varieties, collected from eight districts (Dhaka, Gazipur, Dinajpur, Joypurhat, Pabna, Sirajgonj, Kushtia and Chuadanga) of Bangladesh. Fungi were isolated following tissue planting method. The isolated fungi were Alternaria alternata, A. triticina, Arthirinium sp., Aspergillus flavus, A. fumigatus, A. niger, A. terreus, Aspergillus sp., Bipolaris cynodontis, B. oryzae, B. sorokiniana, B. tetramera, B. victoriae, Bispora antenata, Chaetomium globosum, Chaetophoma sp., Cladosporium cladosporioides, Coniothyrium sp., Curvularia affinis, C. lunata, C. pallescens, Drechslera dematioidea, D. hawaiiensis, Epicoccum purpurascens, Eurotium sp., Fusarium moniliforme, F. nivale, F. semitectum, Nigrospora oryzae, N. sacchari, Penicillium digitatum, Pestalotiopsis guepinii, Rhizopus stolonifer, Syncephalastrum racemosum and Trichoderma viride. Bispora antenata is new record for Bangladesh. Dhaka Univ. J. Biol. Sci. 28(1): 21-35, 2019 (January)

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Evaluation of Different Pretreatment Processes of Lignocellulosic Biomass for Enhanced Biomethane Production

Energy & Fuels ◽

10.1021/acs.energyfuels.7b02045 ◽

2017 ◽

Vol 31 (10) ◽

pp. 10335-10347 ◽

Cited By ~ 26

Author(s):

Samer Dahadha ◽

Zeid Amin ◽

Amir Abbas Bazyar Lakeh ◽

Elsayed Elbeshbishy

Keyword(s):

Lignocellulosic Biomass ◽

Biomethane Production ◽

Pretreatment Processes

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Enzymatic Hydrolysis of Lignocellulosic Biomass Using an Optimized Enzymatic Cocktail Prepared From Secretomes of Filamentous Fungi Isolated From Amazonian Biodiversity

10.21203/rs.3.rs-460730/v1 ◽

2021 ◽

Author(s):

Pamella Santa Rosa ◽

Jessica Batista de Oliveira ◽

Spartaco Astolfi Filho ◽

Nei Pereira

Keyword(s):

Lignocellulosic Biomass ◽

Plant Biomass ◽

Protein G ◽

Enzyme Extract ◽

Cellulose Pulp ◽

Enzyme Cocktail ◽

Hydrolysis Of Cellulose ◽

Aspergillus Sp ◽

Hydrolysis Of ◽

Cassava Peel

Abstract The use of lignocellulosic biomass (LCB) has emerged as one of the main strategies for generating renewable biofuels. For the efficient use of such feedstock, pretreatments are essential. The hydrolysis of cellulose – major component of LCB - demands enzymatic cocktails with improved efficiency to generate fermentable sugars. In this scenario, lignocellulolytic fungi have enormous potential for the development of efficient enzyme platforms. In this study, two enzymatic cocktails were developed for hydrolysis of two lignocellulosic biomasses: industrial cellulose pulp and cassava peel. The solid biomass ratio in relation to the protein content of the enzyme cocktail were performed by experimental design. The optimized cocktail for the hydrolysis of cellulose pulp (AMZ 1) was composed, in protein base, by 43% of Aspergillus sp LMI03 enzyme extract and 57% of T. reesei QM9414, while the optimal enzyme cocktail for cassava peel hydrolysis (AMZ 2) was composed by 50% of Aspergillus sp LMI03 enzyme extract, 25% of the extract of P. citrinum LMI01 and 25% of T. reesei. The ratio between solids and protein loading for AMZ 1 cocktail performance was 52 g/L solids and 30mg protein/g solids, resulting in a hydrolytic efficiency of 93%. For the AMZ 2 cocktail, the hydrolytic efficiency was 78% for an optimized ratio of 78g/L solids and 19mg protein/g solids. These results indicate that cocktails formulated with enzymatic extracts of P. citrinum LMI01, Aspergillus sp LMI03 and T. reesei QM9414 are excellent alternatives for efficient hydrolysis of plant biomass and for other processes that depend on biocatalysis.

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Characterization and Application of Aspergillus tubingensis USMI03 RPf10 Fermented Solution as a Nutrient Source for Microbial Growth

South Asian Journal of Research in Microbiology ◽

10.9734/sajrm/2020/v6i230144 ◽

2020 ◽

pp. 1-9

Author(s):

Shimaa A. Amin ◽

Hemmat M. Abdelhady ◽

Khadiga A. Abou-Taleb ◽

Zahra H. Tayeb ◽

Rania F. Ahmed

Keyword(s):

Growth Rate ◽

Indole Acetic Acid ◽

Microbial Growth ◽

Trichoderma Viride ◽

Aspergillus Tubingensis ◽

Nutrient Source ◽

E Coli ◽

Aspergillus Sp ◽

Rhizopus Sp ◽

Synthetic Media

The fermented solution of this strain contains the highest concentration of citric acid, indole acetic acid (IAA) and elements as well as phosphatase activity. Using the Aspergillus tubingensis USMI03 RPf10 fermented solution as a medium led to decrease in the growth rate of tested bacterial isolates (Bacillus subtilis, Pseudomonas fluorescens and Escherichia coli) and fungal isolates (Aspergillus sp. and Penicillium sp.) except Trichoderma viride which gave the same growth on fermented solution treatments compared to control. Whereas the tested yeast isolates (Candida olivera and Saccharomyces cerevisiae) recorded the same growth rate on fermented solution treatments as whole media and higher growth rate on fermented solution + glucose treatment, compared to control. Also, the latter treatment resulting in higher growth of some tested bacteria (B. subtilis and P. fluorescens) and the same growth rate of other bacteria (E. coli), compared to synthetic media. The growth of Rhizopus sp. decreased on fermented solution treatments than control. It is suggested that, using A. tubingensis USMI03 RPf10 fermented solution as a whole medium or as a mineral could be used as a source for microbial growth which varied from one microorganism to another.

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