Distinguishing cytosine methylation using electrochemical, label-free detection of DNA hybridization and ds-targets

2015 ◽  
Vol 64 ◽  
pp. 74-80 ◽  
Author(s):  
Bicheng Zhu ◽  
Marsilea A. Booth ◽  
Phillip Shepherd ◽  
Allan Sheppard ◽  
Jadranka Travas-Sejdic
Keyword(s):  
Dna Hybridization ◽  
Cytosine Methylation ◽  
Label Free ◽  
Electrochemical Label ◽  
Label Free Detection

scholarly journals Label-free detection of morpholino-DNA hybridization using a silicon photonics suspended slab micro-ring resonator

2021 ◽  
pp. 1-1
Author(s):  
Soha Yousuf ◽  
Jongmin Kim ◽  
Ajymurat Orozaliev ◽  
Marcus Dahlem ◽  
Yong-Ak Song ◽  
...  
Keyword(s):  
Silicon Photonics ◽  
Dna Hybridization ◽  
Ring Resonator ◽  
Label Free ◽  
Label Free Detection

Label-Free Detection of DNA Hybridization Utilizing Dual S-Tapered Thin-Core Fiber Interferometer

2019 ◽  
Vol 37 (11) ◽  
pp. 2762-2767 ◽  
Author(s):  
Xu Zhang ◽  
Bo Liu ◽  
Hao Zhang ◽  
Xu Zhang ◽  
Binbin Song ◽  
...  
Keyword(s):  
Dna Hybridization ◽  
Label Free ◽  
Label Free Detection ◽  
Core Fiber

scholarly journals A Graphene-Based Glycan Biosensor for Electrochemical Label-Free Detection of a Tumor-Associated Antibody

Sensors ◽  
2019 ◽  
Vol 19 (24) ◽  
pp. 5409 ◽  
Author(s):  
Filip Kveton ◽  
Anna Blsakova ◽  
Lenka Lorencova ◽  
Monika Jerigova ◽  
Dusan Velic ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Secondary Ion Mass Spectrometry ◽  
Covalent Immobilization ◽  
Label Free ◽  
Surface Plasmon Resonance Analysis ◽  
Binding Preference ◽  
Electrochemical Label ◽  
Label Free Detection

The study describes development of a glycan biosensor for detection of a tumor-associated antibody. The glycan biosensor is built on an electrochemically activated/oxidized graphene screen-printed electrode (GSPE). Oxygen functionalities were subsequently applied for covalent immobilization of human serum albumin (HSA) as a natural nanoscaffold for covalent immobilization of Thomsen-nouvelle (Tn) antigen (GalNAc-O-Ser/Thr) to be fully available for affinity interaction with its analyte—a tumor-associated antibody. The step by step building process of glycan biosensor development was comprehensively characterized using a battery of techniques (scanning electron microscopy, atomic force microscopy, contact angle measurements, secondary ion mass spectrometry, surface plasmon resonance, Raman and energy-dispersive X-ray spectroscopy). Results suggest that electrochemical oxidation of graphene SPE preferentially oxidizes only the surface of graphene flakes within the graphene SPE. Optimization studies revealed the following optimal parameters: activation potential of +1.5 V vs. Ag/AgCl/3 M KCl, activation time of 60 s and concentration of HSA of 0.1 g L−1. Finally, the glycan biosensor was built up able to selectively and sensitively detect its analyte down to low aM concentration. The binding preference of the glycan biosensor was in an agreement with independent surface plasmon resonance analysis.

Label free detection of DNA hybridization by refractive index tapered fiber biosensor

2010 ◽  
Author(s):  
M. I. Zibaii ◽  
H. Latifi ◽  
E. Ghanati ◽  
M. Gholami ◽  
S. M. Hosseini
Keyword(s):  
Refractive Index ◽  
Dna Hybridization ◽  
Label Free ◽  
Tapered Fiber ◽  
Label Free Detection

scholarly journals Silicene Quantum Capacitance Dependent Frequency Readout to a Label-free Detection of DNA Hybridization

2021 ◽  
Author(s):  
Sazzadur Rahman ◽  
Rokaia Laizu Naima ◽  
Khatuna Jannatun Shetu ◽  
Mahabub Hossain ◽  
M. Shamim Kaiser ◽  
...  
Keyword(s):  
Band Gap ◽  
Dna Hybridization ◽  
Glucose Sensor ◽  
Detection Method ◽  
Genetic Research ◽  
Ph Sensor ◽  
Detection Methods ◽  
Quantum Capacitance ◽  
Label Free ◽  
Label Free Detection

Two-dimensional silicon allotrodes– also called Sinicene– have recently experienced intensive scientific research interest due to their unique electrical, mechanical, and sensing characteristics. A novel silicene based nano-material has been enticed great amenities, partially because of its uniformity with graphene. Silicene is a highly sensitive for numerous sensors based on molecular sensing as pH sensor, gas sensor, ion sensor and biosensing are Deoxyribonucleic acid (DNA) nucleobase sensor, photonic sensor, cell-based biosensor, glucose sensor, and bioelectric nose sensor. Nowadays genetic research based on DNA hybridization, which is a vital tools for sensing material and it has various detection methods. Among of them, the detection method is frequency readout used to a label-free detection of DNA hybridization. In this paper we have compared the graphene and silicene quantum capacitance that has been proposed for a DNA hybridization detection method on wireless readout. These method shows, the strands of mismatched and complementary DNA have in different range of frequency to identify output efficiency. With respect to DNA concentration the output of silicene is almost sharply linear than graphene. In addition of field effect transistor, silicene opens a new opportunities due to its band gap whereas graphene indicates zero band gap. It can be stated that silicene is much more reliable as well as much stronger than multi-layered graphene.

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