Membrane filter-assisted surface enhanced Raman spectroscopy for the rapid detection of E. coli O157:H7 in ground beef

2015 ◽  
Vol 64 ◽  
pp. 171-176 ◽  
Author(s):  
Il-Hoon Cho ◽  
Pushpak Bhandari ◽  
Pradip Patel ◽  
Joseph Irudayaraj
Keyword(s):  
Raman Spectroscopy ◽  
Rapid Detection ◽  
Membrane Filter ◽  
Ground Beef ◽  
Surface Enhanced Raman Spectroscopy ◽  
E Coli ◽  
Surface Enhanced ◽  
Surface Enhanced Raman

Bacterial detection and differentiation via direct volatile organic compound sensing with surface enhanced Raman spectroscopy

2017 ◽  
Author(s):  
Caitlin S. DeJong ◽  
David I. Wang ◽  
Aleksandr Polyakov ◽  
Anita Rogacs ◽  
Steven J. Simske ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Bacterial Infections ◽  
Direct Detection ◽  
Point Of Care ◽  
Surface Enhanced Raman Spectroscopy ◽  
E Coli ◽  
Recent Emergence ◽  
Surface Enhanced ◽  
Volatile Organic ◽  
Surface Enhanced Raman

Through the direct detection of bacterial volatile organic compounds (VOCs), via surface enhanced Raman spectroscopy (SERS), we report here a reconfigurable assay for the identification and monitoring of bacteria. We demonstrate differentiation between highly clinically relevant organisms: <i>Escherichia coli</i>, <i>Enterobacter cloacae</i>, and <i>Serratia marcescens</i>. This is the first differentiation of bacteria via SERS of bacterial VOC signatures. The assay also detected as few as 10 CFU/ml of <i>E. coli</i> in under 12 hrs, and detected <i>E. coli</i> from whole human blood and human urine in 16 hrs at clinically relevant concentrations of 10<sup>3</sup> CFU/ml and 10<sup>4</sup> CFU/ml, respectively. In addition, the recent emergence of portable Raman spectrometers uniquely allows SERS to bring VOC detection to point-of-care settings for diagnosing bacterial infections.

Understanding the competitive interactions in aptamer–gold nanoparticle based colorimetric assays using surface enhanced Raman spectroscopy (SERS)

2016 ◽  
Vol 8 (7) ◽  
pp. 1602-1608 ◽  
Author(s):  
Shintaro Pang ◽  
Lili He
Keyword(s):  
Raman Spectroscopy ◽  
Gold Nanoparticle ◽  
Rapid Detection ◽  
Surface Enhanced Raman Spectroscopy ◽  
Competitive Interactions ◽  
Detection Methods ◽  
Color Changes ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Colorimetric Assays

Aptamer–gold nanoparticle (AuNP) based colorimetric assays have become increasingly popular as viable rapid detection methods, but the molecular interactions governing the mechanism and successful interpretation of color changes have not been explored well.

Rapid Detection of Carbapenemase-Producing Enterobacteriacae Based on Surface-Enhanced Raman Spectroscopy with Gold Nanostars

2020 ◽  
Vol 6 (5) ◽  
pp. 947-953 ◽  
Author(s):  
Yen Lynn Wong ◽  
Wei Cherng Malvin Kang ◽  
Miguel Reyes ◽  
Jeanette Woon Pei Teo ◽  
James Chen Yong Kah
Keyword(s):  
Raman Spectroscopy ◽  
Rapid Detection ◽  
Surface Enhanced Raman Spectroscopy ◽  
Gold Nanostars ◽  
Surface Enhanced ◽  
Surface Enhanced Raman

Rapid Detection of an Anthrax Biomarker by Surface-Enhanced Raman Spectroscopy

2005 ◽  
Vol 127 (12) ◽  
pp. 4484-4489 ◽  
Author(s):  
Xiaoyu Zhang ◽  
Matthew A. Young ◽  
Olga Lyandres ◽  
Richard P. Van Duyne
Keyword(s):  
Raman Spectroscopy ◽  
Rapid Detection ◽  
Surface Enhanced Raman Spectroscopy ◽  
Surface Enhanced ◽  
Surface Enhanced Raman
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