Sensitive detection of active Shiga toxin using low cost CCD based optical detector

2015 ◽  
Vol 68 ◽  
pp. 705-711 ◽  
Author(s):  
Reuven Rasooly ◽  
Josh Balsam ◽  
Bradley J. Hernlem ◽  
Avraham Rasooly
Keyword(s):  
Shiga Toxin ◽  
Low Cost ◽  
Sensitive Detection ◽  
Optical Detector

Highly sensitive detection of Cr(vi) in groundwater by bimetallic NiFe nanoparticles

2017 ◽  
Vol 9 (6) ◽  
pp. 1031-1037 ◽  
Author(s):  
Jingtao Liu ◽  
Yu Ding ◽  
Lifei Ji ◽  
Xin Zhang ◽  
Fengchun Yang ◽  
...  
Keyword(s):  
Heavy Metal ◽  
Hexavalent Chromium ◽  
Low Cost ◽  
High Sensitivity ◽  
Sensitive Detection ◽  
Toxic Heavy Metal ◽  
Metal Pollutants ◽  
Highly Sensitive ◽  
Highly Sensitive Detection ◽  
Heavy Metal Pollutants

Hexavalent chromium (Cr(vi)) is one of the most toxic heavy metal pollutants in groundwater, and thus the detection of Cr(vi) with high sensitivity, accuracy, and simplicity and low cost is of great importance.

Highly Selective and Sensitive Detection of Arsenite Ions (III) Using a Novel Tetraphenylimidazole-based Probe

2021 ◽  
Author(s):  
Rong Song ◽  
Yeshuo Ma ◽  
Anyao Bi ◽  
Bin Feng ◽  
Liu Huang ◽  
...  
Keyword(s):  
Low Cost ◽  
Arsenic Concentration ◽  
Sensitive Detection ◽  
The World ◽  
Living Species

More than 200 million people in the world are exposed to areas where the arsenic concentration exceeds the limit allowed for living species, which urges people to develop low-cost methods...

scholarly journals Development of Low-Cost AuNP-Based Aptasensors with Truncated Aptamer for Highly Sensitive Detection of 8-Oxo-dG in Urine

ACS Omega ◽  
2020 ◽  
Vol 5 (28) ◽  
pp. 17423-17430
Author(s):  
Piyaporn Matulakul ◽  
Drusawin Vongpramate ◽  
Sirinan Kulchat ◽  
Apiwat Chompoosor ◽  
Raynoo Thanan ◽  
...  
Keyword(s):  
Low Cost ◽  
Sensitive Detection ◽  
Highly Sensitive ◽  
Highly Sensitive Detection

scholarly journals Molecular Risk Assessment and Epidemiological Typing of Shiga Toxin-ProducingEscherichia coliby Using a Novel PCR Binary Typing System

2011 ◽  
Vol 77 (7) ◽  
pp. 2458-2470 ◽  
Author(s):  
Stephanie M. Brandt ◽  
Nicola King ◽  
Angela J. Cornelius ◽  
Aruni Premaratne ◽  
Thomas E. Besser ◽  
...  
Keyword(s):  
Risk Assessment ◽  
Shiga Toxin ◽  
Diarrheal Disease ◽  
Diversity Index ◽  
Low Cost ◽  
Severe Disease ◽  
Epidemiological Studies ◽  
Health Concern ◽  
Pfge Typing ◽  
Cluster Dendrogram

ABSTRACTShiga toxin-producingEscherichia coli(STEC) is a zoonotic pathogen that causes diarrheal disease in humans and is of public health concern because of its ability to cause outbreaks and severe disease such as hemorrhagic colitis or hemolytic-uremic syndrome. More than 400 serotypes of STEC have been implicated in outbreaks and sporadic human disease. The aim of this study was to develop a PCR binary typing (P-BIT) system that could be used to aid in risk assessment and epidemiological studies of STEC by using gene targets that would represent a broad range of STEC virulence genes. We investigated the distribution of 41 gene targets in 75 O157 and non-O157 STEC isolates and found that P-BIT provided 100% typeability for isolates, gave a diversity index of 97.33% (compared with 99.28% for XbaI pulsed-field gel electrophoresis [PFGE] typing), and produced 100% discrimination for non-O157 STEC isolates. We identified 24 gene targets that conferred the same level of discrimination and produced the same cluster dendrogram as the 41 gene targets initially examined. P-BIT clustering identified O157 from non-O157 isolates and identified seropathotypes associated with outbreaks and severe disease. Numerical analysis of the P-BIT data identified several genes associated with human or nonhuman sources as well as high-risk seropathotypes. We conclude that P-BIT is a useful approach for subtyping, offering the advantage of speed, low cost, and potential for strain risk assessment that can be used in tandem with current molecular typing schema for STEC.

Versatile low‐cost digital lock‐in amplifier suitable for multichannel phase‐sensitive detection

1989 ◽  
Vol 60 (7) ◽  
pp. 2257-2259 ◽  
Author(s):  
S. Carrato ◽  
G. Paolucci ◽  
R. Tommasini ◽  
R. Rosei
Keyword(s):  
Low Cost ◽  
Sensitive Detection ◽  
Phase Sensitive ◽  
Phase Sensitive Detection ◽  
Lock In

scholarly journals Effect of Citrus Byproducts on Survival of O157:H7 and Non-O157Escherichia coliSerogroups withinIn VitroBovine Ruminal Microbial Fermentations

2013 ◽  
Vol 2013 ◽  
pp. 1-5 ◽  
Author(s):  
Heather A. Duoss-Jennings ◽  
Ty B. Schmidt ◽  
Todd R. Callaway ◽  
Jeffery A. Carroll ◽  
James M. Martin ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Shiga Toxin ◽  
General Trend ◽  
Low Cost ◽  
Nutritional Supplement ◽  
Variant Form ◽  
Fluid Media

Citrus byproducts (CBPs) are utilized as a low cost nutritional supplement to the diets of cattle and have been suggested to inhibit the growth of bothEscherichia coliO157:H7 andSalmonella. The objective of this study was to examine the effectsin vitrothat varying concentrations of CBP in the powdered or pelleted variety have on the survival of Shiga-toxinEscherichia coli(STEC) serotypes O26:H11, O103:H8, O111:H8, O145:H28, and O157:H7 in bovine ruminal microorganism media. The O26:H11, O111:H8, O145:H28, and O157:H7 serotypes did not exhibit a change in populations in media supplemented with CBP with either variety. The O103:H8 serotype displayed a general trend for an approximate1log10reduction in 5% powdered CBP and 20% pelleted CBP over 6 h. There was a trend for reductions in populations of a variant form of O157:H7 mutated in thestx1 andstx2 genes in higher concentrations of CBP. These results suggest that variations exist in the survival of these serotypes of STEC within mixed ruminal microorganism fluid media when supplemented with CBP. Further research is needed to determine why CBPs affect STEC serotypes differently.

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