A method employing SPE, MRM LC-MS/MS and a THF–water solvent system for the simultaneous determination of five antiretroviral drugs in human blood plasma

2017 ◽  
Vol 9 (3) ◽  
pp. 450-458 ◽  
Author(s):  
Elliott Mwando ◽  
Amos Massele ◽  
Enoch Sepako ◽  
Kwenga Sichilongo
Keyword(s):  
Blood Plasma ◽  
Simultaneous Determination ◽  
Human Blood ◽  
Multiple Reaction Monitoring ◽  
Solvent System ◽  
Antiretroviral Drugs ◽  
Human Blood Plasma ◽  
Liquid Chromatography Mass Spectrometry ◽  
Water Solvent

A multiple reaction monitoring liquid chromatography – mass spectrometry method for the simultaneous determination of five antiretroviral drugs in human blood plasma.

scholarly journals Optimization of a method for the determination of a mustard gas biomarker in human blood plasma by liquid chromatography–mass spectrometry

2017 ◽  
Vol 1 (3) ◽  
pp. 7-17
Keyword(s):  
Blood Plasma ◽  
Human Blood ◽  
Standard Addition ◽  
Proteinase K ◽  
Human Blood Plasma ◽  
Mustard Gas ◽  
Liquid Chromatography Mass Spectrometry ◽  
Tandem Mass Spectrometric ◽  
Hydrolysis Of

A method for the determination of a mustard gas biomarker (an S-hydroxyethylthioethyl adduct with albumin) in blood plasma was optimized with the use of HPLC with tandem mass-spectrometric detection. This method is based on the hydrolysis of this adduct by the proteinase K enzyme with the formation of the following stable tripeptide with cysteine, proline, and phenylalanine: S-HETE-Cys-Pro-Phe. The detection limit of mustard gas in human blood plasma was 2 ng/ml. The approach proposed was tested in the analysis of human blood plasma samples by the standard addition technique and also within the framework of the first official biomedical test carried out by the Organization for the Prohibition of Chemical Weapons (OPCW) in 2016, and it exhibited a good accuracy, reproducibility, and specificity of determination.

Simultaneous determination of aromatic amino acids in human blood plasma by capillary electrophoresis with UV-absorption detection

2015 ◽  
Vol 38 (10) ◽  
pp. 1794-1799 ◽  
Author(s):  
Mauro Forteschi ◽  
Salvatore Sotgia ◽  
Stefano Assaretti ◽  
Dionigia Arru ◽  
Debora Cambedda ◽  
...  
Keyword(s):  
Amino Acids ◽  
Capillary Electrophoresis ◽  
Blood Plasma ◽  
Simultaneous Determination ◽  
Human Blood ◽  
Aromatic Amino Acids ◽  
Uv Absorption ◽  
Human Blood Plasma

Simultaneous determination of CRP and D-dimer in human blood plasma samples with White Light Reflectance Spectroscopy

2016 ◽  
Vol 84 ◽  
pp. 89-96 ◽  
Author(s):  
Georgios Koukouvinos ◽  
Panagiota Petrou ◽  
Konstantinos Misiakos ◽  
Dimitris Drygiannakis ◽  
Ioannis Raptis ◽  
...  
Keyword(s):  
Blood Plasma ◽  
Simultaneous Determination ◽  
Human Blood ◽  
White Light ◽  
Reflectance Spectroscopy ◽  
Human Blood Plasma ◽  
Plasma Samples ◽  
Light Reflectance ◽  
D Dimer

scholarly journals Development and validation of an improved HPLC-UV method for simultaneous determination of lamotrigine and oxcarbazepine and its active metabolite 10,11-dihydro-10-hydroxycarbazepine in human blood plasma and comparison with an UHPLC-MS/MS method

2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Siyao Jin ◽  
Qing Zhao ◽  
Dongjie Zhang ◽  
Zhigang Zhao ◽  
Shenghui Mei
Keyword(s):  
Liquid Chromatography ◽  
Blood Plasma ◽  
Simultaneous Determination ◽  
Human Blood ◽  
High Performance ◽  
Internal Standard ◽  
Ultra Performance Liquid Chromatography ◽  
Human Blood Plasma ◽  
Elution Time

AbstractLamotrigine (LTG) and oxcarbazepine (OXC) are first-line drugs for epilepsy treatment. Their large pharmacokinetics variabilities and relations between efficacy and toxicity and blood plasma concentration require routine monitoring for dose adjustment. In this study, we developed and validated a simple, accurate, and reliable method for simultaneous determination of LTG, OXC and 10,11-dihydro-10-hydroxycarbazepine (MHD) in human blood plasma by high-performance liquid chromatography-ultraviolet detection (HPLC-UV) with a simple one-step protein precipitation using methanol (1% acetic acid) and 15 min elution time under isocratic elution at 1 mL/min. Calibration range was 2.4 to 120 mg/L for LTG, OXC, and MHD. The intra-day and inter-day bias were − 8.84 to 4.18%, and the imprecision was less than 8.08% for all analytes. The internal standard (fluconazole) normalized recovery was 96.30 to 107.69% for LTG, 98.51 to 111.04% for MHD, and 95.04 to 109.86% for OXC. A total of 186 LTG samples and 25 MHD samples were used to evaluate the agreement between HPLC-UV and ultra-performance liquid chromatography-mass spectrometry (UHPLC-MS/MS) by Passing-Bablok regression and Bland-Altman plot. The mean bias and the 95% limits of agreement (95% LOA) of the two measurements were 0.575 mg/L and − 1.238 to 2.387 mg/L for LTG (n = 186) and − 1.222 mg/L and − 8.271 to 5.827 mg/L for MHD (n = 25), which indicated the UV method was comparable with the MS method for LTG and MHD analysis.

scholarly journals A novel LC–ESI-MS method for the simultaneous determination of etravirine, darunavir and ritonavir in human blood plasma

Talanta ◽  
2009 ◽  
Vol 79 (5) ◽  
pp. 1372-1378 ◽  
Author(s):  
Naser L. Rezk ◽  
Nicole R. White ◽  
Steven H. Jennings ◽  
Angela D.M. Kashuba
Keyword(s):  
Blood Plasma ◽  
Simultaneous Determination ◽  
Human Blood ◽  
Human Blood Plasma ◽  
Esi Ms
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