A novel label-free electrochemical immunosensor based on the enhanced catalytic currents of oxygen reduction by AuAg hollow nanocrystals for detecting carbohydrate antigen 199

2017 ◽  
Vol 96 ◽  
pp. 152-158 ◽  
Author(s):  
Rui Wang ◽  
Jiu-Ju Feng ◽  
Wei-Dong Liu ◽  
Liu-Ying Jiang ◽  
Ai-Jun Wang
Keyword(s):  
Oxygen Reduction ◽  
Carbohydrate Antigen ◽  
Electrochemical Immunosensor ◽  
Label Free

scholarly journals A Label-Free Electrochemical Immunosensor for Detection of the Tumor Marker CA242 Based on Reduced Graphene Oxide-Gold-Palladium Nanocomposite

Nanomaterials ◽  
2019 ◽  
Vol 9 (9) ◽  
pp. 1335 ◽  
Author(s):  
Xin Du ◽  
Xiaodi Zheng ◽  
Zhenhua Zhang ◽  
Xiaofan Wu ◽  
Lei Sun ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Tumor Marker ◽  
High Performance ◽  
Synthesis Method ◽  
Carbohydrate Antigen ◽  
Electrochemical Immunosensor ◽  
Redox Activity ◽  
Label Free ◽  
Early Cancer Diagnosis ◽  
Reduction Cycle

As a tumor marker, carbohydrate antigen 24-2 (CA242) is a highly accurate and specific diagnostic indicator for monitoring pancreatic and colorectal cancers. The goal of this study was to create a novel label-free electrochemical immunosensor using a nanocomposite glassy carbon electrode for the detection of CA242. Graphene oxide (GO) and polyvinyl pyrrolidone were chosen as the dopants for the preparation of a high-performance reduced-GO-gold-palladium (rGO-Au-Pd) nanocomposite. RGO-Au-Pd was characterized using X-ray diffraction and transmission electron microscopy, revealing that the material exhibited superior electrochemical redox activity and electron transfer ability. The effects of the synthesis method, material concentration, reduction cycle, and pH were investigated to optimize the performance of the immunosensor. As a result of the catalytic activity and biocompatibility of rGO-Au-Pd, the prepared CA242 immunosensor displayed a wide linear range of detection from 0.001 U/mL to 10,000 U/mL with a detection limit of 1.54 × 10−3 U/mL and a sensitivity of 4.24 μA (log10CCA242)−1. More importantly, the immunosensor exhibited satisfactory reproducibility and selectivity when detected CA242 in PBS or human serum. The results of our study provide a platform for the development of novel bioassays for use in early cancer diagnosis and promote the application of biosensing technology in the medical field.

scholarly journals Construction of a Label-Free Electrochemical Immunosensor Based on Zn-Co-S/Graphene Nanocomposites for Carbohydrate Antigen 19-9 Detection

Nanomaterials ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1475
Author(s):  
Chia-Wei Su ◽  
Jia-Hao Tian ◽  
Jin-Jia Ye ◽  
Han-Wei Chang ◽  
Yu-Chen Tsai
Keyword(s):  
Transition Metal ◽  
Limit Of Detection ◽  
Direct Reduction ◽  
Metal Sulfide ◽  
Carbohydrate Antigen ◽  
Electrochemical Immunosensor ◽  
Label Free ◽  
Graphene Nanocomposites ◽  
Transition Metal Sulfide ◽  
Electrochemical Signal

Nanocomposites of the binary transition metal sulfide Zn-Co-S/graphene (Zn-Co-S@G) were synthesized through a one-step hydrothermal method. They may be useful in the construction of an electrochemical immunosensor for carbohydrate antigen 19-9 (CA19-9) detection. Zn-Co-S dot-like nanoparticles uniformly covered the surface of graphene to form an interconnected conductive network, ensuring strong interaction between transition metal sulfide and graphene, which can expose numerous electroactive sites leading to the improvement of the amplified electrochemical signal toward a direct reduction of H2O2. Thus, the construction of an electrochemical immunosensor using Zn-Co-S@G nanocomposites showed outstanding sensing properties for detecting CA19-9. The constructed electrochemical immunosensor exhibited a good linear relationship in the range of 6.3 U·mL−1–300 U·mL−1, with the limit of detection at 0.82 U·mL−1, which makes it a promising candidate for an electrochemical immunosensor.

Electrochemical Immunosensors Based on Nanostructured Materials for Sensing of Prostate-Specific Antigen: A Review

2020 ◽  
Vol 28 ◽  
Author(s):  
Hayati Filik ◽  
Asiye Aslıhan Avan ◽  
Mustafa Özyürek
Keyword(s):  
Prostate Specific Antigen ◽  
Nanostructured Materials ◽  
Specific Antigen ◽  
Electrochemical Immunosensor ◽  
Label Free ◽  
Electrochemical Immunosensors ◽  
Metal Metal ◽  
Different Types ◽  
Sandwich Type ◽  
Carbon Based Nanomaterials

: The prostate-specific antigen (PSA) has been considered a crucial serological marker for distinguishing prostate based cancer. This surveys recent progress in the construction of nanomaterial-based electrochemical immunosensors for a PSA. This review (from 2015 to 2020) reports the latest progress in PSA sensing based on the employ of different types of nanostructured materials. The most popular used nanostructured materials are metal, metal oxide, carbon-based nanomaterials, and their hybrid architectures utilized for distinct amplification protocols. In this review, the electrochemical immunosensors for prostate-specific antigen sensing are classified into three categories such as sandwich type@labeled, label free@nonlabeled and aptamer-based electrochemical immunosensor.

scholarly journals Ultrasensitive and label free electrochemical immunosensor for detection of ROR1 as an oncofetal biomarker using gold nanoparticles assisted LDH/rGO nanocomposite

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Rozita Abolhasan ◽  
Balal Khalilzadeh ◽  
Hadi Yousefi ◽  
Sahar Samemaleki ◽  
Forough Chakari-Khiavi ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Flexible Structures ◽  
Modified Electrodes ◽  
Electrochemical Immunosensor ◽  
Lymphocytic Leukemia ◽  
Orphan Receptor ◽  
Detection Sensitivity ◽  
Label Free ◽  
Serum Samples ◽  
Limit Of Quantification

AbstractIn the present article, we developed a highly sensitive label-free electrochemical immunosensor based on NiFe-layered double hydroxides (LDH)/reduced graphene oxide (rGO)/gold nanoparticles modified glassy carbon electrode for the determination of receptor tyrosine kinase-like orphan receptor (ROR)-1. In this electrochemical immunoassay platform, NiFe-LDH/rGO was used due to great electron mobility, high specific surface area and flexible structures, while Au nanoparticles were prepared and coated on the modified electrodes to improve the detection sensitivity and ROR1 antibody immobilizing (ROR1Ab). The modification procedure was approved by using cyclic voltammetry and differential pulse voltammetry based on the response of peak current to the step by step modifications. Under optimum conditions, the experimental results showed that the immunosensor revealed a sensitive response to ROR1 in the range of 0.01–1 pg mL−1, and with a lower limit of quantification of 10 attogram/mL (10 ag mL−1). Furthermore, the designed immunosensor was applied for the analysis of ROR1 in several serum samples of chronic lymphocytic leukemia suffering patients with acceptable results, and it also exhibited good selectivity, reproducibility and stability.

Construction of electrochemical immunosensor based on redox hydrogel for ultrasensitive detection of carcinoembryonic antigen

2021 ◽  
Author(s):  
Xiangrong Huang ◽  
Na Wu ◽  
Wenxiu Liu ◽  
Yazhuo Shang ◽  
Honglai Liu ◽  
...  
Keyword(s):  
Methylene Blue ◽  
Carcinoembryonic Antigen ◽  
Electrochemical Detection ◽  
Electrochemical Immunosensor ◽  
Ultrasensitive Detection ◽  
Label Free

In this work, a novel redox hydrogel was proposed for ultrasensitive label-free electrochemical detection of carcinoembryonic antigen (CEA). The redox hydrogel composed by cellulose nanocrystalline (CNC), methylene blue (MB), multi-walled...

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