DaimonDNA: A portable, low-cost loop-mediated isothermal amplification platform for naked-eye detection of genetically modified organisms in resource-limited settings

2019 ◽  
Vol 141 ◽  
pp. 111409 ◽  
Author(s):  
Doğukan Kaygusuz ◽  
Sümeyra Vural ◽  
Ali Özhan Aytekin ◽  
Stuart James Lucas ◽  
Meltem Elitas
2021 ◽  
Vol 21 (4) ◽  
pp. 2123-2131
Author(s):  
Alagan Jeevika ◽  
Dhesingh Ravi Shankaran

A simple, low-cost and highly selective nanosensor was developed for naked-eye detection of mercury ions (Hg2+) based on Eosin/silver nanocubes (Eosin/AgNCbs). Silver nanocubes (AgNCbs) were synthesized by polyol assisted chemical method. HR-TEM result shows the formed AgNCbs have a mean diameter of 84±0.005 nM (diagonally measured) and edge length of 55±0.01 nM. XRD result confirms that the AgNCbs are single crystalline in nature with a phase structure of face centered cubic (FCC) of silver. On interaction of Hg2+, AgNCbs exhibits a color change from gray to black up to 16.67 μM of Hg2+ owed to the formation of solid like bimetallic complex of Ag/Hg amalgam. The selectivity of AgNCbs was evaluated with several other toxic metal ions including, Mg2+, Ba2+, Ca4+, Pb2+, Cd4+, Zn2+, Co2+, Cu2+, K+ and Ni2+ and found good selectivity towards Hg2+. The sensitivity of the AgNCbs sensor system was tuned by using Eosin as a co-staining agent. The Eosin/AgNCbs showed a limit of detection of 60±0.050 nM with the color change from orange to purple. The results suggests that the Eosin/AgNCbs nanosensor exhibits good selectivity, sensitivity, repeatability and rapid response, which could be explored for real-time detection of Hg2+ in environmental and biological samples.


The Analyst ◽  
2015 ◽  
Vol 140 (3) ◽  
pp. 931-937 ◽  
Author(s):  
Sharifun Nahar ◽  
Minhaz Uddin Ahmed ◽  
Mohammadali Safavieh ◽  
Annie Rochette ◽  
Carla Toro ◽  
...  

Effective viral detection is a key goal in the development of point of care (POC) diagnostic devices.


2017 ◽  
Vol 22 (4) ◽  
pp. 406-412 ◽  
Author(s):  
Jessica T. Wen ◽  
Polrit Viravathana ◽  
Brian Ingel ◽  
Caroline Roper ◽  
Hideaki Tsutsui

This study presents a sensor strip for user-friendly, naked-eye detection of Xylella fasitdiosa, the bacterial causal agent of Pierce’s disease in grapevine. This sensor uses anti– X. fastidiosa antibodies conjugated to a polydiacetylene layer on a polyvinylidene fluoride strip to generate specific color transitions and discriminate levels of the pathogen. The detection limit of the sensor is 0.8 × 108 cells/mL, which is similar to bacterial load in grapevine 18 days following bacterial inoculation. This sensor enables equipment-free detection that is highly desirable for in-field diagnostic tools in resource-limited settings.


Author(s):  
Aron Hakonen ◽  
Niklas Strömberg

Drinking water contamination of lead from various environmental sources, leaching consumer products and intrinsic water-pipe infrastructure is still today a matter of great concern. Therefore, new highly sensitive and convenient Pb2+ measurement schemes are necessary, especially for in-situ measurements at a low-cost. Within this work dye/ionophore/Pb2+ co-extraction and effective water phase de-colorization was utilized for highly sensitive lead measurements and sub-ppb naked-eye detection. A low-cost ionophore Benzo-18-Crown-6-ether was used, and a simple test-tube mix and separate procedure was developed. Instrumental detection limits were in the low ppt region (LOD=3, LOQ=10), and naked-eye detection was 500 ppt. Note, however, that this sensing scheme still has improvement potential as concentrations of fluorophore and ionophore were not optimized. Artificial tap-water samples, leached by a standardized method, demonstrated drinking water application. Implications for this method are convenient in-situ lead ion measurements.


2019 ◽  
Vol 7 (36) ◽  
pp. 5461-5464 ◽  
Author(s):  
Haiyang Lin ◽  
Xin Wang ◽  
Jiahui Wu ◽  
Haiyin Li ◽  
Feng Li

Dopamine-functionalized cellulose paper with low cost, portable, and disposable features was applied for an equipment-free and naked-eye detection of transduction factor based on the enzyme-assisted signal amplification/transduction reaction.


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