Dietary calcium supplementation stimulates bone formation and inhibits bone resorption after bone marrow ablation in mice

AbstractBone marrow ablation prompts transient bone formation in nearly the entire medullary cavity before marrow regeneration occurs. Here, we establish a procedure to direct bone formation in a desired particular site within the medullary cavity for support of biomedical devices. Local intramedullary injury was performed in the tibiae of rats and parathyroid hormone (PTH), alendronate, or saline was administered. Newly generated bone in the medulla was assessed by micro-CT and histology. To evaluate the function of newly generated bone, animals received intramedullary injury in tibiae followed by daily PTH. At day-14, implants were placed in the endocortical bone and the bone response to the implants was assessed. The fate of newly generated bone was compared with and without implants. We found that neither intramedullary injury nor medication alone resulted in bone formation. However, when combined, substantial bone was generated locally inside the diaphyseal medulla. Newly formed bone disappeared without implant placement but was retained with implants. Bone was especially retained around and between the implants. This study found that local bone marrow disruption followed by PTH or alendronate generated substantial cancellous bone locally in the diaphyseal medulla. This approach offers promise as a tissue engineering tool in medicine and dentistry.

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Retardation in Bone Resorption after Bone Marrow Ablation in Klotho Mutant Mice1

Endocrinology ◽

10.1210/endo.141.1.7252 ◽

2000 ◽

Vol 141 (1) ◽

pp. 438-445 ◽

Cited By ~ 17

Author(s):

Teruhito Yamashita ◽

Hiroyuki Yoshitake ◽

Kunikazu Tsuji ◽

Nanako Kawaguchi ◽

Yo-ichi Nabeshima ◽

...

Keyword(s):

Bone Marrow ◽

Bone Resorption ◽

Bone Marrow Ablation ◽

Marrow Ablation

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Dietary Calcium Supplementation Suppresses Bone Formation in Magnesium-Deficient Rats

International Journal for Vitamin and Nutrition Research ◽

10.1024/0300-9831.76.3.111 ◽

2006 ◽

Vol 76 (3) ◽

pp. 111-116 ◽

Cited By ~ 3

Author(s):

Hiroshi Matsuzaki ◽

Misao Miwa

Keyword(s):

Bone Formation ◽

Calcium Supplementation ◽

Control Diet ◽

Formation Rate ◽

Dietary Calcium ◽

Mineral Apposition Rate ◽

Control Group ◽

Deficient Diet ◽

Bone Formation Rate ◽

Male Wistar Rats

The purpose of this study was to clarify the effects of dietary calcium (Ca) supplementation on bone metabolism of magnesium (Mg)-deficient rats. Male Wistar rats were randomized by weight into three groups, and fed a control diet (control group), a Mg-deficient diet (Mg- group) or a Mg-deficient diet having twice the control Ca concentrations (Mg-2Ca group) for 14 days. Trabecular bone volume was significantly lower in the Mg - and Mg-2Ca groups than in the control group. Trabecular number was also significantly lower in the Mg - and Mg-2Ca groups than in the control group. Mineralizing bone surface, mineral apposition rate (MAR), and surface referent bone formation rate (BFR/BS) were significantly lower in the Mg - and Mg-2Ca groups than in the control group. Furthermore, MAR and BFR/BS were significantly lower in the Mg-2Ca group than in the Mg - group. These results suggest that dietary Ca supplementation suppresses bone formation in Mg-deficient rats.

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153Sm EDTMP for bone marrow ablation prior to stem cell transplantation for haematological malignancies

Nuclear Medicine Communications ◽

10.1097/00006231-200211000-00010 ◽

2002 ◽

Vol 23 (11) ◽

pp. 1099-1106 ◽

Cited By ~ 14

Author(s):

D. J. MACFARLANE ◽

S. DURRANT ◽

M. L. BARTLETT ◽

R. ALLISON ◽

A. J. MORTON

Keyword(s):

Bone Marrow ◽

Stem Cell ◽

Stem Cell Transplantation ◽

Cell Transplantation ◽

Haematological Malignancies ◽

Bone Marrow Ablation ◽

Marrow Ablation

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Aged Mice Require Full Transcription Factor, Runx2/Cbfa1, Gene Dosage for Cancellous Bone Regeneration After Bone Marrow Ablation

Journal of Bone and Mineral Research ◽

10.1359/jbmr.040601 ◽

2004 ◽

Vol 19 (9) ◽

pp. 1481-1489 ◽

Cited By ~ 29

Author(s):

Kunikazu Tsuji ◽

Toshihisa Komori ◽

Masaki Noda

Keyword(s):

Bone Marrow ◽

Transcription Factor ◽

Bone Regeneration ◽

Cancellous Bone ◽

Gene Dosage ◽

Aged Mice ◽

Bone Marrow Ablation ◽

Marrow Ablation

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Human serum and plasma have different sources of epidermal growth factor

AJP Regulatory Integrative and Comparative Physiology ◽

10.1152/ajpregu.1990.259.3.r545 ◽

1990 ◽

Vol 259 (3) ◽

pp. R545-R548 ◽

Cited By ~ 2

Author(s):

A. Lev-Ran ◽

D. L. Hwang ◽

D. S. Snyder

Keyword(s):

Bone Marrow ◽

Platelet Count ◽

Growth Factor ◽

Epidermal Growth Factor ◽

Molecular Mass ◽

High Performance ◽

Regression Line ◽

Bone Marrow Ablation ◽

Epidermal Growth ◽

Marrow Ablation

Epidermal growth factor (EGF) was determined by radioimmunoassay in serum, plasma, and urine of 23 patients undergoing ablative therapy followed by bone marrow transplantation. The difference between the serum and plasma values reflected the amount of EGF released from the platelets at the time of blood coagulation. Platelet-derived EGF strongly correlated with platelet count (r + 0.850, P less than 0.0001), and the intercept of the regression line was very close to zero; one platelet contained approximately 2.5 x 10(-18) g EGF. Correspondingly, when the platelet count dropped after bone marrow ablation from 222 +/- 97 to 33 +/- 13 x 10(9)/l, the serum EGF decreased from 603 +/- 222 to 65 +/- 41 pg/ml (P less than 0.0001). Plasma EGF content did not correlate with the platelet count and did not change significantly after bone marrow ablation (before and after the ablation, correspondingly, 290 +/- 80 and 332 +/- 99 pg/ml, P = 0.194). High-performance liquid chromatographic fractionation of serum and plasma showed different molecular mass distribution of EGF-immunoreactive fractions. The main molecular mass components of the plasma EGF did not change after bone marrow ablation. Urine excretion remained unchanged (320 +/- 133 and 314 +/- 173 pmol EGF/mmol creatinine). We conclude that whereas platelets are the source of serum EGF, the origin of plasma EGF is different and the search of its origin is warranted.

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