scholarly journals Dual-Color Single Plane Illumination Fluorescence Correlation Spectroscopy (SPIM-FCS) using a Single Photon Detector and Hardware Based Image Processing

2012 ◽  
Vol 102 (3) ◽  
pp. 207a ◽  
Author(s):  
Jan Buchholz ◽  
Jan W. Krieger ◽  
Agata Pernus ◽  
Edoardo Charbon ◽  
Udo Kebschull ◽  
...  
2014 ◽  
Vol 22 (23) ◽  
pp. 28783 ◽  
Author(s):  
Taro Yamashita ◽  
Dengkuan Liu ◽  
Shigehito Miki ◽  
Johtaro Yamamoto ◽  
Tokuko Haraguchi ◽  
...  

2019 ◽  
Author(s):  
Ipsita Saha ◽  
Saveez Saffarian

AbstractWe present a method utilizing single photon interference and fluorescence correlation spectroscopy (FCS) to simultaneously measure transport of fluorescent molecules within aqueous samples. Our method, within seconds, measures transport in thousands of homogenous voxels (100 nm)3 and eliminates photo-physical artifacts associated with blinking of fluorescent molecules. A comprehensive theoretical framework is presented and validated by measuring transport of quantum dots, associated with VSV-G receptor along cellular membranes as well as within viscous gels.


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