Interferometric Fluorescent Cross Correlation Spectroscopy

Cross Correlation ◽

Single Photon ◽

Theoretical Framework ◽

Aqueous Samples ◽

Cellular Membranes ◽

Fluorescent Molecules

AbstractWe present a method utilizing single photon interference and fluorescence correlation spectroscopy (FCS) to simultaneously measure transport of fluorescent molecules within aqueous samples. Our method, within seconds, measures transport in thousands of homogenous voxels (100 nm)3 and eliminates photo-physical artifacts associated with blinking of fluorescent molecules. A comprehensive theoretical framework is presented and validated by measuring transport of quantum dots, associated with VSV-G receptor along cellular membranes as well as within viscous gels.

Effect of Capping Agent and Medium on Light-Induced Variation of the Luminescence Properties of CdTe Quantum Dots: A Study Based on Fluorescence Correlation Spectroscopy, Steady State and Time-Resolved Fluorescence Techniques

The Journal of Physical Chemistry C ◽

10.1021/jp5048216 ◽

2014 ◽

Vol 118 (31) ◽

pp. 18187-18196 ◽

Cited By ~ 15

Author(s):

Satyajit Patra ◽

Anunay Samanta

Keyword(s):

Quantum Dots ◽

Steady State ◽

Cdte Quantum Dots ◽

Capping Agent ◽

Time Resolved Fluorescence ◽

Time Resolved ◽

Fluorescence Techniques

1P508 Detection of prion protein immune complex using Fluorescence Correlation Spectroscopy and Fluorescence Cross-Correlation Spectroscopy(25. New methods and tools (I),Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)

Seibutsu Butsuri ◽

10.2142/biophys.46.s273_4 ◽

2006 ◽

Vol 46 (supplement2) ◽

pp. S273

Author(s):

Fumihiko Fujii ◽

Motohiro Horiuchi ◽

Masayoshi Ueno ◽

Hiroshi Sakata ◽

Issei Nagao ◽

...

Keyword(s):

Prion Protein ◽

Immune Complex ◽

Cross Correlation ◽

Poster Session ◽

New Methods ◽

Meeting Program

Diffusion of fluorescent molecules and quantum dot nanoparticles in viscous solvents investigated by fluorescence correlation spectroscopy

Vietnam Journal of Chemistry ◽

10.1002/vjch.201800035 ◽

2018 ◽

Vol 56 (4) ◽

pp. 488-492

Author(s):

Nguyen Thi Thanh Bao ◽

Dinh Van Trung ◽

Nguyen Thanh Binh ◽

Vu Thi Bich ◽

Ung Thi Dieu Thuy ◽

...

Keyword(s):

Quantum Dot ◽

Viscous Solvents ◽

Fluorescent Molecules

‘True’ single-molecule molecule observations by fluorescence correlation spectroscopy and two-color fluorescence cross-correlation spectroscopy

Experimental and Molecular Pathology ◽

10.1016/j.yexmp.2006.12.002 ◽

2007 ◽

Vol 82 (2) ◽

pp. 147-155 ◽

Cited By ~ 8

Author(s):

Zeno Földes-Papp

Keyword(s):

Single Molecule ◽

Cross Correlation ◽

Detection of prion protein immune complex for bovine spongiform encephalopathy diagnosis using fluorescence correlation spectroscopy and fluorescence cross-correlation spectroscopy

Analytical Biochemistry ◽

10.1016/j.ab.2007.07.018 ◽

2007 ◽

Vol 370 (2) ◽

pp. 131-141 ◽

Cited By ~ 28

Author(s):

Fumihiko Fujii ◽

Motohiro Horiuchi ◽

Masayoshi Ueno ◽

Hiroshi Sakata ◽

Issei Nagao ◽

...

Keyword(s):

Bovine Spongiform Encephalopathy ◽

Prion Protein ◽

Immune Complex ◽

Cross Correlation ◽

Spongiform Encephalopathy ◽

Studies on Interaction of CdTe Quantum Dots with Bovine Serum Albumin Using Fluorescence Correlation Spectroscopy

Journal of Fluorescence ◽

10.1007/s10895-008-0396-0 ◽

2008 ◽

Vol 19 (1) ◽

pp. 151-157 ◽

Cited By ~ 34

Author(s):

Liwen Shao ◽

Chaoqing Dong ◽

Fuming Sang ◽

Huifeng Qian ◽

Jicun Ren

Keyword(s):

Quantum Dots ◽

Bovine Serum Albumin ◽

Serum Albumin ◽

Bovine Serum ◽

Cdte Quantum Dots ◽

Dual-Color Single Plane Illumination Fluorescence Correlation Spectroscopy (SPIM-FCS) using a Single Photon Detector and Hardware Based Image Processing

Biophysical Journal ◽

10.1016/j.bpj.2011.11.1130 ◽

2012 ◽

Vol 102 (3) ◽

pp. 207a ◽

Cited By ~ 1

Author(s):

Jan Buchholz ◽

Jan W. Krieger ◽

Agata Pernus ◽

Edoardo Charbon ◽

Udo Kebschull ◽

...

Keyword(s):

Image Processing ◽

Single Photon ◽

Photon Detector ◽

Single Photon Detector ◽

Single Plane ◽

Dual Color

Size determination of quantum dots with fluorescence correlation spectroscopy

10.1117/12.874660 ◽

2011 ◽

Cited By ~ 1

Author(s):

D. Hill ◽

C. Ast ◽

H.-G. Löhmannsröben ◽

A. Zulqurnain ◽

W. Parak ◽

...

Keyword(s):

Quantum Dots ◽

Size Determination ◽

Fluorescence correlation spectroscopy and fluorescence cross-correlation spectroscopy reveal the cytoplasmic origination of loaded nuclear RISC in vivo in human cells

Nucleic Acids Research ◽

10.1093/nar/gkn693 ◽

2008 ◽

Vol 36 (20) ◽

pp. 6439-6449 ◽

Cited By ~ 121

Author(s):

Thomas Ohrt ◽

Jörg Mütze ◽

Wolfgang Staroske ◽

Lasse Weinmann ◽

Julia Höck ◽

...

Keyword(s):

Cross Correlation ◽

Human Cells ◽

Development of a fluorescence correlation spectroscopy instrument and its application in sizing quantum dot nanoparticles

Communications in Physics ◽

10.15625/0868-3166/25/1/5670 ◽

2015 ◽

Vol 25 (1) ◽

pp. 59 ◽

Cited By ~ 1

Author(s):

Nguyen Thi Thanh Bao ◽

Dinh Van Trung

Keyword(s):

Quantum Dots ◽

Dynamic Properties ◽

Fluorescence Signal ◽

Statistical Fluctuations ◽

Measurement Results ◽

Particle Level ◽

Microscope Imaging

Fluorescence correlation spectroscopy is a relatively new technique to measure and quantify the statistical fluctuations of the fluorescence signal from the measurement volume. Combining with sensitive detection method and confocal microscopy, the FCS technique has become a powerful tool in studying the dynamic properties of nanoparticles at single particle level. In this paper we present the construction of a highly sensitive FCS instrument and the measurement results from a sample of semiconductor quantum dots. We provide the analysis procedure for determining the hydrodynamic radius of the quantum dots and compare the results with that obtained directly from electron microscope imaging. The good agreement indicates the reliability of the FCS technique and open the way for further applications of this technique in studying nanoparticles.