Influence of varying concentrations of chitosan coating on the pore wall of polycaprolactone based porous scaffolds for tissue engineering application

Current strategies of regenerative medicine are focused on the restoration of pathologically altered tissue architectures by transplantation of cells in combination with supportive scaffolds and biomolecules. In recent years, considerable interest has been given to biologically active scaffolds which are based on similar analogs of the extracellular matrix that have induced synthesis of tissues and organs. To restore function or regenerate tissue, a scaffold is necessary that will act as a temporary matrix for cell proliferation and extracellular matrix deposition, with subsequent ingrowth until the tissues are totally restored or regenerated. Scaffolds have been used for tissue engineering such as bone, cartilage, ligament, skin, vascular tissues, neural tissues, and skeletal muscle and as vehicle for the controlled delivery of drugs, proteins, and DNA. Various technologies come together to construct porous scaffolds to regenerate the tissues/organs and also for controlled and targeted release of bioactive agents in tissue engineering applications. In this paper, an overview of the different types of scaffolds with their material properties is discussed. The fabrication technologies for tissue engineering scaffolds, including the basic and conventional techniques to the more recent ones, are tabulated.

Download Full-text

Fabrication of porous scaffolds with decellularized cartilage matrix for tissue engineering application

Biologicals ◽

10.1016/j.biologicals.2017.05.008 ◽

2017 ◽

Vol 48 ◽

pp. 39-46 ◽

Cited By ~ 15

Author(s):

Bita Nasiri ◽

Shohreh Mashayekhan

Keyword(s):

Tissue Engineering ◽

Engineering Application ◽

Cartilage Matrix ◽

Porous Scaffolds ◽

Tissue Engineering Application

Download Full-text

Optimization and evaluation of silk fibroin-chitosan freeze-dried porous scaffolds for cartilage tissue engineering application

Journal of Biomaterials Science Polymer Edition ◽

10.1080/09205063.2016.1148303 ◽

2016 ◽

Vol 27 (7) ◽

pp. 657-674 ◽

Cited By ~ 29

Author(s):

Varshini Vishwanath ◽

Krishna Pramanik ◽

Amit Biswas

Keyword(s):

Tissue Engineering ◽

Silk Fibroin ◽

Cartilage Tissue Engineering ◽

Engineering Application ◽

Cartilage Tissue ◽

Porous Scaffolds ◽

Tissue Engineering Application ◽

Freeze Dried

Download Full-text

Effects of Hydroxyapatite/Silk Fibroin/Chitosan Ratio on Physical Properties of Scaffold for Tissue Engineering Application

Materials Science Forum ◽

10.4028/www.scientific.net/msf.872.261 ◽

2016 ◽

Vol 872 ◽

pp. 261-265 ◽

Cited By ~ 1

Author(s):

Wassanai Wattanutchariya ◽

Atitaya Oonjai ◽

Kittiya Thunsiri

Keyword(s):

Tissue Engineering ◽

Physical Properties ◽

Pore Structure ◽

Silk Fibroin ◽

Engineering Application ◽

Porous Scaffolds ◽

Tissue Engineering Application ◽

Pass Through ◽

Near Future ◽

Interconnected Pore

This study reports the effects of the mixing ratio of hydroxyapatite (HA), silk fibroin (SF) and chitosan (CS) on the physical properties of the scaffold used in tissue engineering. Experimental design based on mixture design was implemented to investigate the degradation rate of the scaffolds fabricated from various ratios of those biomaterials. Furthermore, pore morphology and pore size were evaluated to confirm the compatibility of the scaffold topography for cell growth and adhesion. The results from the study showed that all ratios, except pure HA solution, can be fabricated into porous scaffolds with an interconnected pore structure and appropriate pore sizes to allow all types of human cells to pass through. Furthermore, the scaffold solutions with high CS ratio resulted in a uniform pore structure and lower rates of biodegradation. Therefore, CS is recommended as the main structure because it provides the highest resistance to biodegradation. The scaffolds from various ratios may be applied for different tissue replacements in the near future.

Download Full-text

Electrospun Nanofibers and their Application in Tissue Repair and Engineering

Journal of Shahid Sadoughi University of Medical Sciences ◽

10.18502/ssu.v27i11.2491 ◽

2020 ◽

Author(s):

Sareh Arjmand ◽

Alireza Partovi Baghdadeh ◽

Amin Hamidi ◽

Seyed Omid Ranaei Siadat

Keyword(s):

Tissue Engineering ◽

Extracellular Matrix ◽

High Surface ◽

Engineering Application ◽

Volume Ratio ◽

Biologically Active ◽

Dimensional Structure ◽

Porous Scaffolds ◽

Tissue Engineering Application ◽

Effective Parameters

Introduction: Tissue engineering is the repair and replacement of damaged tissues and requires a combination of cells, growth factor and porous scaffolds. Scaffolds, as one of the main components in tissue engineering, are used as a template for tissue regeneration and induction and guidance of growth of the new and biologically active tissues. An ideal scaffold in tissue engineering, imitating an extracellular matrix, provides a suitable environment for adhesion, growth and cell proliferation. Scaffolds have also been used as the carriers for the controlled delivery of drugs and proteins. Variety of porous scaffolds, fabricated from biological and synthetic materials and using different manufacturing methods, have been introduced. Among them nanofibrous scaffolds have attracted great attention due to remarkable advantages including the highly porous three-dimensional structure with interconnected cavities which enable the transportation of food and waste materials, as well as high surface to volume ratio. So far, different methods and techniques have been introduced for production of scaffolds with structures similar to the extracellular matrix. Amongst them electrospinning, due to easiness and more control over effective parameters, are preferred. The present study make a review about the used materials and various methods of nanofibrous scaffold fabrication using electrospinning technology, with emphasis on the use of tissue engineering application. It also discussed about the progress and challenges ahead and the goals and perspective presented for this approach.

Download Full-text