Shotgun proteomics analysis reveals sub-lethal effects in Daphnia magna exposed to cell-bound microcystins produced by Microcystis aeruginosa

2020 ◽  
Vol 33 ◽  
pp. 100656
Author(s):  
René S. Shahmohamadloo ◽  
Denina B.D. Simmons ◽  
Paul K. Sibley
Keyword(s):  
Daphnia Magna ◽  
Microcystis Aeruginosa ◽  
Shotgun Proteomics ◽  
Proteomics Analysis ◽  
Lethal Effects

Sub-lethal effects of Microcystis aeruginosa on Daphnia magna after an acute exposure

2000 ◽  
Vol 27 (4) ◽  
pp. 2107-2110
Author(s):  
Piedade Barros ◽  
M. Leonor Fidalgo ◽  
Amadeu M. V. M. Soares
Keyword(s):  
Daphnia Magna ◽  
Microcystis Aeruginosa ◽  
Acute Exposure ◽  
Lethal Effects

scholarly journals An Improvement of Shotgun Proteomics Analysis by Adding Next-Generation Sequencing Transcriptome Data in Orange

PLoS ONE ◽  
2012 ◽  
Vol 7 (6) ◽  
pp. e39494 ◽  
Author(s):  
Jiaping Song ◽  
Renjie Sun ◽  
Dazhi Li ◽  
Fengji Tan ◽  
Xin Li ◽  
...  
Keyword(s):  
Next Generation Sequencing ◽  
Shotgun Proteomics ◽  
Transcriptome Data ◽  
Next Generation ◽  
Proteomics Analysis ◽  
Generation Sequencing

scholarly journals Shotgun Proteomics Analysis of Saliva and Salivary Gland Tissue from the Common Octopus Octopus vulgaris

2018 ◽  
Vol 17 (11) ◽  
pp. 3866-3876 ◽  
Author(s):  
Legana C. H. W. Fingerhut ◽  
Jan M. Strugnell ◽  
Pierre Faou ◽  
Álvaro Roura Labiaga ◽  
Jia Zhang ◽  
...  
Keyword(s):  
Salivary Gland ◽  
Shotgun Proteomics ◽  
Octopus Vulgaris ◽  
Proteomics Analysis ◽  
Salivary Gland Tissue ◽  
Gland Tissue ◽  
The Common ◽  
Common Octopus

Shotgun proteomics analysis of proliferating STRO-1-positive human dental pulp cell after exposure to nacreous water-soluble matrix

2014 ◽  
Vol 19 (2) ◽  
pp. 261-270 ◽  
Author(s):  
Titikan Laothumthut ◽  
Jeeraphat Jantarat ◽  
Atchara Paemanee ◽  
Sittiruk Roytrakul ◽  
Panjit Chunhabundit
Keyword(s):  
Dental Pulp ◽  
Shotgun Proteomics ◽  
Water Soluble ◽  
Proteomics Analysis ◽  
Pulp Cell ◽  
Human Dental Pulp Cell ◽  
Dental Pulp Cell ◽  
Human Dental Pulp

scholarly journals Application of Biotests in Cyanobacterial Extract Toxicity Assessment

2014 ◽  
Vol 40 (3) ◽  
pp. 115-121 ◽  
Author(s):  
Anna Sierosławska ◽  
Anna Rymuszka ◽  
Tadeusz Skowroński
Keyword(s):  
Acute Toxicity ◽  
Daphnia Magna ◽  
Microcystis Aeruginosa ◽  
Tetrahymena Thermophila ◽  
Toxicity Assessment ◽  
Taxonomic Identification ◽  
Dam Reservoir ◽  
Extract Concentration ◽  
Initial Extract ◽  
Toxicity Unit

Abstract The aim of the study was to determine the toxicity of the extract obtained from the cyanobacterial cells derived from the waters of Zemborzycki dam reservoir with use of a battery of biotests. The taxonomic identification of the bloom-forming cyanobacteria revealed high abundance of Aphanizomenon flos-aquae and Dolichospermum spp. (Anabaena spp.) and in a lower degree of Microcystis aeruginosa and Planktothrix agardhii. In the extract obtained from concentrated cyanobacterial cells, hepatotoxin microcystin-LR at a concentration of 22.89 ± 3.74 μg/L and neurotoxin Antx-a at 13.02 ± 0.01 μg/L have been detected. Toxicity of the extract was evaluated with the following assays: Daphtoxkit F magna with the crustacean Daphnia magna, Thamnotoxkit F with the crustacean Thamnocephalus platyurus, Rotoxkit F with the rotifer Brachionus calyciflorus and Protoxkit F with ciliate Tetrahymena thermophila. The most sensitive organism among all studied was T. platyurus for which EC50 was estimated to be 1.2% of the initial extract concentration. On the basis of the highest obtained value of the toxicity unit (TU = 83) the studied sample was classified to the IV class, which is of high acute toxicity. Additionally, it was found that reactivity on cyanobacterial products differs greatly among organisms used in bioassays, which indicate the need for using a set of biotests.

Optimizing Shotgun Proteomics Analysis for a Confident Protein Identification and Quantitation in Orphan Plant Species: The Case of Holm Oak (Quercus ilex)

2020 ◽  
pp. 157-168
Author(s):  
Isabel Gómez-Gálvez ◽  
Rosa Sánchez-Lucas ◽  
Bonoso San-Eufrasio ◽  
Luis Enrique Rodríguez de Francisco ◽  
Ana M. Maldonado-Alconada ◽  
...  
Keyword(s):  
Plant Species ◽  
Protein Identification ◽  
Quercus Ilex ◽  
Shotgun Proteomics ◽  
Holm Oak ◽  
Proteomics Analysis ◽  
Protein Identification And Quantitation

Shotgun proteomics analysis reveals new unsuspected molecular effectors of nitrogen-containing bisphosphonates in osteocytes

2011 ◽  
Vol 74 (7) ◽  
pp. 1113-1122 ◽  
Author(s):  
Nicoletta Bivi ◽  
Paola Picotti ◽  
Lukas N. Müller ◽  
Milena Romanello ◽  
Luigi Moro ◽  
...  
Keyword(s):  
Shotgun Proteomics ◽  
Proteomics Analysis

scholarly journals Acute toxic effects of the cyanobacterium Microcystis aeruginosa on Daphnia magna 1

1986 ◽  
Vol 31 (3) ◽  
pp. 497-502 ◽  
Author(s):  
Shulamit Nizan ◽  
Chanan Dimentman ◽  
Moshe Shilo
Keyword(s):  
Daphnia Magna ◽  
Microcystis Aeruginosa ◽  
Toxic Effects ◽  
Acute Toxic
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