Lobster hepatopancreatic epithelial single cell suspensions as models for electrogenic sodium–proton exchange

The development of high efficient stacks is critical for the wide spread application of proton exchange membrane fuel cells (PEMFCs) in transportation and stationary power plant. Currently, the favorable operation conditions of PEMFCs are with single cell voltage between 0.65 and 0.7 V, corresponding to energy efficiency lower than 57%. For the long term, PEMFCs need to be operated at higher voltage to increase the energy efficiency and thus promote the fuel economy for transportation and stationary applications. Herein, PEMFC single cell was investigated to demonstrate its capability to working with voltage and energy efficiency higher than 0.8 V and 65%, respectively. It was demonstrated that the PEMFC encountered a significant performance degradation after the 64 h operation. The cell voltage declined by more than 13% at the current density of 1000 mA cm−2, due to the electrode de-activation. The high operation potential of the cathode leads to the corrosion of carbon support and then causes the detachment of Pt nanoparticles, resulting in significant Pt agglomeration. The catalytic surface area of cathode Pt is thus reduced for oxygen reduction and the cell performance decreased. Therefore, electrochemically stable Pt catalyst is highly desirable for efficient PEMFCs operated under cell voltage higher than 0.8 V.

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Generation of three-dimensional pannus-like tissues in vitro from single cell suspensions of synovial fluid cells from arthritis patients

Rheumatology International ◽

10.1007/s00296-003-0333-0 ◽

2004 ◽

Vol 24 (2) ◽

pp. 71-76 ◽

Cited By ~ 1

Author(s):

Samuel Solomon ◽

Madhan Masilamani ◽

Subhasis Mohanty ◽

J�rg E. Schwab ◽

Eva-Maria Boneberg ◽

...

Keyword(s):

Synovial Fluid ◽

Single Cell ◽

Three Dimensional ◽

Cell Suspensions ◽

Synovial Fluid Cells

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Sodium/Proton Exchange in the Maintenance of Intracellular pH in FRTL-5 Thyroid Cells*

Endocrinology ◽

10.1210/endo-123-4-1705 ◽

1988 ◽

Vol 123 (4) ◽

pp. 1705-1711 ◽

Cited By ~ 7

Author(s):

CLAUDIO MARCOCCI ◽

EVELYN F. GROLLMAN

Keyword(s):

Intracellular Ph ◽

Proton Exchange ◽

Thyroid Cells ◽

Sodium Proton Exchange

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Intracellular pH recovery and lactate efflux in mouse soleus muscles stimulatedin vitro:the involvement of sodium/proton exchange and a lactate carrier

Acta Physiologica Scandinavica ◽

10.1111/j.1748-1716.1988.tb08340.x ◽

1988 ◽

Vol 132 (3) ◽

pp. 363-371 ◽

Cited By ~ 67

Author(s):

C. JUEL

Keyword(s):

Intracellular Ph ◽

Proton Exchange ◽

Sodium Proton Exchange

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Preparation of Single Cell Suspensions of the Intra-Epithelial Layer and Lamina Propria from Human Intestinal Tissue v1

10.17504/protocols.io.bwq7pdzn ◽

2021 ◽

Author(s):

Steven B. Wells ◽

Pranay Dogra ◽

Josh Gray ◽

Peter A. Szabo ◽

Daniel Caron ◽

...

Keyword(s):

Single Cell ◽

Lamina Propria ◽

Intestinal Tract ◽

Distal Colon ◽

Cell Suspensions ◽

Epithelial Layer ◽

Dilution Factor ◽

Human Gut ◽

Intestinal Tissue ◽

Defined Media

This protocol describes a method for the isolation of the immune cells, structural and epithelial cells, and progenitors from the epithelial layer and the lamina propria of human gut sections of about one gram of tissue. By providing defined media formulations, volumes at each step, and a defined dilution factor for density centrifugation, it yields consistent single-cell suspensions across samples. This protocol can be used for any section of the intestinal tract from duodenum to distal colon.

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Pancreatic beta-cell electrical activity: the role of anions and the control of pH

AJP Cell Physiology ◽

10.1152/ajpcell.1983.244.3.c188 ◽

1983 ◽

Vol 244 (3) ◽

pp. C188-C197 ◽

Cited By ~ 31

Author(s):

G. T. Eddlestone ◽

P. M. Beigelman

Keyword(s):

Membrane Potential ◽

Cell Membrane ◽

Beta Cell ◽

Pancreatic Beta Cell ◽

Proton Exchange ◽

Disulfonic Acid ◽

Control Mechanisms ◽

Exchange System ◽

Sodium Proton Exchange

The influence of chloride on the mouse pancreatic beta-cell membrane potential and the cell membrane mechanisms controlling intracellular pH (pHi) have been investigated using glass microelectrodes to monitor the membrane potential. It has been shown that chloride is distributed passively across the beta-cell membrane such that chloride potential is equal to the membrane potential. Withdrawal of perifusate chloride or bicarbonate and the application of the drugs 4-acetamido-4'-isethiocyanostilbene-2,2'-disulfonic acid (SITS) and probenecid, both blockers of transmembrane anion movement, have been used to establish that a chloride-bicarbonate exchange system is operative in the cell membrane and that it is one of the control mechanisms of pHi. Amiloride, a specific blocker of the transmembrane sodium proton exchange, has been used to demonstrate that this mechanism is also operative in the beta-cell membrane in the control of pHi. The hypothesis that the calcium-activated potassium permeability is proton sensitive at an intracellular site, a fall in pHi causing a fall in permeability and an increase in pHi causing an increase in permeability, has been used to explain many of the effects observed in this study.

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