Efficient removal mechanism for antibiotic resistance genes from aquatic environments by graphene oxide nanosheet

2017 ◽  
Vol 313 ◽  
pp. 836-846 ◽  
Author(s):  
Wenchao Yu ◽  
Sihui Zhan ◽  
Zhiqiang Shen ◽  
Qixing Zhou ◽  
Dong Yang
Keyword(s):  
Antibiotic Resistance ◽  
Graphene Oxide ◽  
Resistance Genes ◽  
Antibiotic Resistance Genes ◽  
Aquatic Environments ◽  
Removal Mechanism ◽  
Efficient Removal ◽  
Graphene Oxide Nanosheet

scholarly journals Characterization of Genetic Determinants Involved in Antimicrobial Resistance in Aeromonas hydrophila, Escherichia coli and Vibrio cholerae Isolated from Different Aquatic Environments

Proceedings ◽  
2020 ◽  
Vol 45 (1) ◽  
pp. 15
Author(s):  
Iroha I. Romanus ◽  
Ude Ibiam ◽  
Ejikeugwu C. Peter ◽  
Onochie C. Chike
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Resistance ◽  
Antimicrobial Resistance ◽  
16S Rrna ◽  
Resistance Genes ◽  
Aeromonas Hydrophila ◽  
Antibiotic Resistance Genes ◽  
Aquatic Environments ◽  
Genetic Determinants ◽  
Specific Primers

Aeromonas hydrophila, Escherichia coli and Vibrio cholerae are among a myriad of bacteria pathogen commonly found in natural water bodies that cause serious waterborne infection while antibiotic resistance genes are emerging contaminants posing potential worldwide human health risk. This study was designed to determined genetic determinants involved in antimicrobial resistance in bacteria isolates from aquatic environments. A total of 372 water samples, comprising of 111, 144 and 117 ponds, rivers and streams were collected from three local governments areas (Abakaliki, Ebonyi and Ikwo) of Ebonyi State Nigeria over a period of twelve (12) months. Bacteria Isolates obtained from water bodies were identified and characterized by polymerase chain reaction (PCR) analysis using 16S rRNA specific primers. The susceptibility of the isolates to different antibiotics was determined using disc diffusion technique. Total DNA was extracted and sequenced on Genetic Analyzer 3130 xl sequencer and the amplified 16S rRNA gene sequence. The presence of antibiotic resistance genes was determined by PCR using specific primers. Bacteria isolated were Aeromonas hydrophila (103), Escherichia coli (118) and Vibrio cholera (87). The isolates were susceptibility to gentamicin (96.12%), meropenem (94.17%), ciprofloxacin (89.32%) imipenem (85.44%) and chloramphenicol (84.47%) while they showed 94.17%, 93.20%, 90.29%, 89.32%, 88.35%, 86.41% and 83.5% resistance to penicillin, sulphamethoxazole, kanamycin, azithromycin, streptomycin cephalothin and cefuroxime respectively. The isolates were found to harbor the following antibiotic resistance genes, blaCTXM, blaSHV, class 1 Integron Ac, Intl1, blaTEM and TetB. These findings deserve serious attention, as the presence of bacteria coliforms in water harbouring antibiotic resistance genes are potential risk to the health of people. Keywords: aquatic environment resistant genes characterization.

scholarly journals Structure of Bacterial Community with Resistance to Antibiotics in Aquatic Environments. A Systematic Review

2021 ◽  
Vol 18 (5) ◽  
pp. 2348
Author(s):  
Ana María Sánchez-Baena ◽  
Luz Dary Caicedo-Bejarano ◽  
Mónica Chávez-Vivas
Keyword(s):  
Systematic Review ◽  
Antibiotic Resistance ◽  
Bacterial Community ◽  
Resistance Genes ◽  
Antibiotic Resistance Genes ◽  
Water Systems ◽  
Anthropogenic Activity ◽  
Aquatic Environments ◽  
Resistance To Antibiotics ◽  
Artificial Water

Aquatic environments have been affected by the increase in bacterial resistant to antibiotics. The aim of this review is to describe the studies carried out in relation to the bacterial population structure and antibiotic resistance genes in natural and artificial water systems. We performed a systematic review based on the PRISMA guideline (preferred reporting items for systematic reviews and meta-analyzes). Articles were collected from scientific databases between January 2010 and December 2020. Sixty-eight papers meeting the inclusion criteria, i.e., “reporting the water bacterial community composition”, “resistance to antibiotics”, and “antibiotic resistance genes (ARG)”, were evaluated according to pre-defined validity criteria. The results indicate that the predominant phyla were Firmicutes and Bacteroidetes in natural and artificial water systems. Gram-negative bacteria of the family Enterobacteraceae with resistance to antibiotics are commonly reported in drinking water and in natural water systems. The ARGs mainly reported were those that confer resistance to β-lactam antibiotics, aminoglycosides, fluoroquinolones, macrolides and tetracycline. The high influence of anthropogenic activity in the environment is evidenced. The antibiotic resistance genes that are mainly reported in the urban areas of the world are those that confer resistance to the antibiotics that are most used in clinical practice, which constitutes a problem for human and animal health.

scholarly journals Diversity of Integron- and Culture-Associated Antibiotic Resistance Genes in Freshwater Floc

2012 ◽  
Vol 78 (12) ◽  
pp. 4367-4372 ◽  
Author(s):  
Christopher N. Drudge ◽  
Amy V. C. Elliott ◽  
Janina M. Plach ◽  
Linda J. Ejim ◽  
Gerard D. Wright ◽  
...  
Keyword(s):  
Trace Elements ◽  
Antibiotic Resistance ◽  
Resistance Gene ◽  
Resistance Genes ◽  
Antibiotic Resistance Gene ◽  
Antibiotic Resistance Genes ◽  
Gene Cassette ◽  
Aquatic Environments ◽  
Resistance Gene Cassette ◽  
Suspended Floc

ABSTRACTClinically important antibiotic resistance genes were detected in culturable bacteria and class 1 integron gene cassettes recovered from suspended floc, a significant aquatic repository for microorganisms and trace elements, across freshwater systems variably impacted by anthropogenic activities. Antibiotic resistance gene cassettes in floc total community DNA differed appreciably in number and type from genes detected in bacteria cultured from floc. The number of floc antibiotic resistance gene cassette types detected across sites was positively correlated with total (the sum of Ag, As, Cu, and Pb) trace element concentrations in aqueous solution and in a component of floc readily accessible to bacteria. In particular, concentrations of Cu and Pb in the floc component were positively correlated with floc resistance gene cassette diversity. Collectively, these results identify suspended floc as an important reservoir, distinct from bulk water and bed sediment, for antibiotic resistance in aquatic environments ranging from heavily impacted urban sites to remote areas of nature reserves and indicate that trace elements, particularly Cu and Pb, are geochemical markers of resistance diversity in this environmental reservoir. The increase in contamination of global water supplies suggests that aquatic environments will become an even more important reservoir of clinically important antibiotic resistance in the future.

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