scholarly journals Simultaneous methane abatement and PHB production by Methylocystis hirsuta in a novel gas-recycling bubble column bioreactor

2018 ◽  
Vol 334 ◽  
pp. 691-697 ◽  
Author(s):  
Teresa García-Pérez ◽  
Juan C. López ◽  
Fabiana Passos ◽  
Raquel Lebrero ◽  
Sergio Revah ◽  
...  
2019 ◽  
Vol 6 (3) ◽  
pp. 84 ◽  
Author(s):  
Moradi ◽  
Rashedi ◽  
Mofradnia ◽  
Khosravi-Darani ◽  
Ashouri ◽  
...  

In this study, the simulation of microorganism ability for the production of poly-β-hydroxybutyrate (PHB) from natural gas (as a carbon source) was carried out. Based on the Taguchi algorithm, the optimum situations for PHB production from natural gas in the columnar bubble reactor with 30 cm length and 1.5 cm diameter at a temperature of 32 °C was evaluated. So, the volume ratio of air to methane of 50:50 was calculated. The simulation was carried out by COMSOL software with two-dimensional symmetric mode. Mass transfer, momentum, density-time, and density-place were investigated. The maximum production of biomass concentration reached was 1.63 g/L, which shows a 10% difference in contrast to the number of experimental results. Furthermore, the consequence of inlet gas rate on concentration and gas hold up was investigated Andres the simulation results were confirmed to experimental results with less than 20% error.


2019 ◽  
Vol 33 (1) ◽  
pp. 69-77 ◽  
Author(s):  
Kianoush Kosravi-Darani ◽  
Fatemeh Yazdian ◽  
Fatemeh Babapour ◽  
A. R. Amirsadeghi

Reducing the total cost of poly(3-hydroxybutyrate) (PHB) production as an attractive substitute for conventional petrochemical plastics still remains an unsolved problem. The aim of this research was the screening of PHB-producing microorganisms and selection of the best suitable medium for microbial growth and PHB production from methane. A new isolated methanotroph for PHB production from natural gas was studied in different media. After selection of the suitable medium, the effect of five process variables (content of nitrogen source, disodium hydrogen phosphate, methane to air ratio, seed age, and pH) on PHB production was investigated in a bubble column bioreactor. Also, hydrodynamic and mass transfer factors (flow regime, mixing time, gas hold up, and kLa) were considered. At optimum operating conditions and engineering parameters in a bubble column, PHB content in the dried biomass reached 25 % w/w. The results showed that pH is the most important variable in the selected conditions.


2019 ◽  
Vol 7 (2) ◽  
pp. 102978 ◽  
Author(s):  
Fatemeh Ghoddosi ◽  
Hossein Golzar ◽  
Fatemeh Yazdian ◽  
Kianoush Khosravi-Darani ◽  
Ebrahim Vasheghani-Farahani

2014 ◽  
Vol 28 (12) ◽  
pp. 7552-7559 ◽  
Author(s):  
Jorge L. S. Sonego ◽  
Diego A. Lemos ◽  
Guilherme Y. Rodriguez ◽  
Antonio J. G. Cruz ◽  
Alberto C. Badino

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