Continuous removal of sulfate and metals from acidic mining-impacted waters at low temperature using a sulfate-reducing bacterial consortium

The objective of this work was to identify one bacterial consortium adapted to the cultivation in the presence of trivalent arsenic (AsIII). Samples were cultured in flasks containing modified Postgate C liquid medium (selective for sulfate-reducing bacteria, SRB). Six different As concentrations were used: 0.5, 1.0, 2.0, 4.0, 8.0 and 16 mg l-1. The growth of sulfate reducing microorganisms was indirectly observed by the formation of an iron sulfide black precipitate and also by the Eh measures.100 ml aliquots of cultured media were centrifuged and stored at-20°C for DNA extraction by phenol/chloroform method. Universal primers 968F-GC 1392R (Bacteria domain) were used for 16S ribosomal DNA amplification. Microbial diversity was evaluated by denaturing gradient gel electrophoresis (DGGE). After DGGE analysis 7 different bands were selected, cut, sequenced and analyzed using the Ribosomal Database Project Release. Consortium microorganisms identified were: Pantoea agglomerans, Enterobacter sp, Citrobacter sp, Cupriavidusmetallidurans, Ralstonia sp, Burkholderia cepacia and Bacillus sp. Thus the microbial consortium here identified is a good candidate for bioremediation of arsenic contaminated areas and effluents.

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Rapid pyritization in the presence of a sulfur/sulfate-reducing bacterial consortium

Scientific Reports ◽

10.1038/s41598-020-64990-6 ◽

2020 ◽

Vol 10 (1) ◽

Cited By ~ 4

Author(s):

Jasmine S. Berg ◽

Arnaud Duverger ◽

Laure Cordier ◽

Christel Laberty-Robert ◽

François Guyot ◽

...

Keyword(s):

Bacterial Consortium ◽

Sulfate Reducing

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The effect of Acetobacter sp. and a sulfate-reducing bacterial consortium from ethanol fuel environments on fatigue crack propagation in pipeline and storage tank steels

Corrosion Science ◽

10.1016/j.corsci.2013.10.036 ◽

2014 ◽

Vol 79 ◽

pp. 128-138 ◽

Cited By ~ 33

Author(s):

J.W. Sowards ◽

C.H.D. Williamson ◽

T.S. Weeks ◽

J.D. McColskey ◽

J.R. Spear

Keyword(s):

Fatigue Crack ◽

Crack Propagation ◽

Fatigue Crack Propagation ◽

Storage Tank ◽

Bacterial Consortium ◽

Ethanol Fuel ◽

Sulfate Reducing ◽

And Storage

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Comparison of Mechanisms of Alkane Metabolism under Sulfate-Reducing Conditions among Two Bacterial Isolates and a Bacterial Consortium

Applied and Environmental Microbiology ◽

10.1128/aem.02896-05 ◽

2006 ◽

Vol 72 (6) ◽

pp. 4274-4282 ◽

Cited By ~ 82

Author(s):

Amy V. Callaghan ◽

Lisa M. Gieg ◽

Kevin G. Kropp ◽

Joseph M. Suflita ◽

Lily Y. Young

Keyword(s):

Bacterial Consortium ◽

Gas Chromatography Mass Spectrometry ◽

Degradation Mechanisms ◽

Bacterial Isolates ◽

Alkane Degradation ◽

Tetradecanoic Acid ◽

Mass Spectral ◽

Sulfate Reducing ◽

Reducing Conditions ◽

Fumarate Addition

ABSTRACT Recent studies have demonstrated that fumarate addition and carboxylation are two possible mechanisms of anaerobic alkane degradation. In the present study, we surveyed metabolites formed during growth on hexadecane by the sulfate-reducing isolates AK-01 and Hxd3 and by a mixed sulfate-reducing consortium. The cultures were incubated with either protonated or fully deuterated hexadecane; the sulfate-reducing consortium was also incubated with [1,2-13C2]hexadecane. All cultures were extracted, silylated, and analyzed by gas chromatography-mass spectrometry. We detected a suite of metabolites that support a fumarate addition mechanism for hexadecane degradation by AK-01, including methylpentadecylsuccinic acid, 4-methyloctadecanoic acid, 4-methyloctadec-2,3-enoic acid, 2-methylhexadecanoic acid, and tetradecanoic acid. By using d 34-hexadecane, mass spectral evidence strongly supporting a carbon skeleton rearrangement of the first intermediate, methylpentadecylsuccinic acid, was demonstrated for AK-01. Evidence indicating hexadecane carboxylation was not found in AK-01 extracts but was observed in Hxd3 extracts. In the mixed sulfate-reducing culture, however, metabolites consistent with both fumarate addition and carboxylation mechanisms of hexadecane degradation were detected, which demonstrates that multiple alkane degradation pathways can occur simultaneously within distinct anaerobic communities. Collectively, these findings underscore that fumarate addition and carboxylation are important alkane degradation mechanisms that may be widespread among phylogenetically and/or physiologically distinct microorganisms.

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Biodegradation of soil-applied polycyclic aromatic hydrocarbons by sulfate-reducing bacterial consortium

Journal of Environmental Science and Health Part A ◽

10.1080/10934520802515178 ◽

2009 ◽

Vol 44 (1) ◽

pp. 12-20 ◽

Cited By ~ 7

Author(s):

Mathava Kumar ◽

Pei-Chi Wu ◽

Jen-Chieh Tsai ◽

Jih-Gaw Lin

Keyword(s):

Polycyclic Aromatic Hydrocarbons ◽

Aromatic Hydrocarbons ◽

Bacterial Consortium ◽

Sulfate Reducing ◽

Polycyclic Aromatic

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The Performance of a Fixed-Bed Anaerobic Bioreactor Using Sulfate-Reducing Bacterial Consortium from Sikidang Crater Sediments

Indonesian Journal of Chemistry ◽

10.22146/ijc.45164 ◽

2019 ◽

Vol 20 (1) ◽

pp. 190

Author(s):

Andriyanto Andriyanto ◽

Wahyu Wilopo ◽

Endah Retnaningrum

Keyword(s):

Ph Value ◽

Fixed Bed ◽

Bacterial Consortium ◽

Ph Profile ◽

Anaerobic Bioreactor ◽

X Ray ◽

Sulfate Reducing ◽

Reduction Efficiency ◽

Bioreactor System ◽

Reducing Bacteria

This research explored the performance of a fixed-bed anaerobic bioreactor system (FBR) using sulfate-reducing bacteria (SRB) from the sediment of Sikidang Crater in Indonesia. Indonesian natural zeolite was used as an inert medium in this bioreactor system. This bioreactor performance was analyzed based on its sulfate reduction efficiency, Cu removal, pH profile, SRB growth, and the changes in mineral composition of the zeolite surface. Based on a batch experiment, the FBR system was operated at 30 °C with a hydraulic retention time (HRT) of 7 days using a zeolite dose of 100 g/L. After its operation, a large amount of SRB (up to 1.5 × 105 cells/mm2) was entrapped and present in the zeolite. This bacterial consortium could reduce sulfate and copper by around 68% and 99.96%, respectively. In addition, the pH value of the bioreactor changed to neutral, which indicated a good performance of the operation. The result of the Energy-Dispersive X-ray (EDX) confirmed that copper removal was caused by the formation of copper-sulfide precipitation. Mapping also revealed that both copper and sulfur were precipitated at the same location.

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Characterization of partial anaerobic metabolic pathway for 2,4,6-trinitrotoluene degradation by a sulfate-reducing bacterial consortium

Canadian Journal of Microbiology ◽

10.1139/m96-155 ◽

1996 ◽

Vol 42 (12) ◽

pp. 1203-1208 ◽

Cited By ~ 23

Author(s):

R. Boopathy ◽

J. F. Manning

Keyword(s):

Acetic Acid ◽

Butyric Acid ◽

Sole Source ◽

Bacterial Consortium ◽

Organic Substrates ◽

Sulfate Reducing ◽

Nitrobenzoic Acid ◽

Metabolic Capability ◽

Terminal Oxidation

The anaerobic degradative pathway for metabolism of 2,4,6-trinitrotoluene (TNT) by a consortium of Desulfovibrio spp. isolated from a creek sediment was studied. This consortium has the metabolic capability to degrade TNT to fatty acids. The growth of the consortium and the metabolism of TNT were greatly enhanced in the presence of an additional carbon source like pyruvate. The optimal concentration of pyruvate for the maximum rate of TNT degradation was 15–20 mM. Various intermediates of TNT metabolism were identified. The first step in the pathway was reduction of TNT to 4-amino-2,6-dinitrotoluene and 2-amino-4,6-dinitrotoluene, which were further reduced to 2,4-diamino,6-nitrotoluene. The next intermediate to appear in the culture medium was nitrobenzoic acid, followed by cyclohexanone, 2-methyl pentanoic acid, butyric acid, and acetic acid. A study using radiolabeled TNT showed that no CO2was produced from TNT during metabolism. The mass balance of the radiolabeled study showed that 49.6% of the TNT was converted to acetic acid, 28% was assimilated into biomass as trichloroacetic acid precipitable materials, and the rest was distributed as various TNT intermediates. Most Desulfovibrio spp. are incomplete oxidizers that are unable to carry out the terminal oxidation of organic substrates. The major end product of TNT metabolism was acetic acid. The bacteria grew on all the TNT intermediates tested as sole source of carbon, except on acetic acid, confirming that the Desulfovibrio spp. have the enzymes necessary for complete degradation of TNT to acetate.Key words: TNT, bioremediation, sulfate reducers, anaerobic process, butyric acid, Desulfovibrio, spp.

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