scholarly journals The Performance of a Fixed-Bed Anaerobic Bioreactor Using Sulfate-Reducing Bacterial Consortium from Sikidang Crater Sediments

2019 ◽  
Vol 20 (1) ◽  
pp. 190
Author(s):  
Andriyanto Andriyanto ◽  
Wahyu Wilopo ◽  
Endah Retnaningrum
Keyword(s):  
Ph Value ◽  
Fixed Bed ◽  
Bacterial Consortium ◽  
Ph Profile ◽  
Anaerobic Bioreactor ◽  
X Ray ◽  
Sulfate Reducing ◽  
Reduction Efficiency ◽  
Bioreactor System ◽  
Reducing Bacteria

This research explored the performance of a fixed-bed anaerobic bioreactor system (FBR) using sulfate-reducing bacteria (SRB) from the sediment of Sikidang Crater in Indonesia. Indonesian natural zeolite was used as an inert medium in this bioreactor system. This bioreactor performance was analyzed based on its sulfate reduction efficiency, Cu removal, pH profile, SRB growth, and the changes in mineral composition of the zeolite surface. Based on a batch experiment, the FBR system was operated at 30 °C with a hydraulic retention time (HRT) of 7 days using a zeolite dose of 100 g/L. After its operation, a large amount of SRB (up to 1.5 × 105 cells/mm2) was entrapped and present in the zeolite. This bacterial consortium could reduce sulfate and copper by around 68% and 99.96%, respectively. In addition, the pH value of the bioreactor changed to neutral, which indicated a good performance of the operation. The result of the Energy-Dispersive X-ray (EDX) confirmed that copper removal was caused by the formation of copper-sulfide precipitation. Mapping also revealed that both copper and sulfur were precipitated at the same location.

Identification of a Bacterial Consortium with Biotechnological Potential for Arsenic Bioremediation

2013 ◽  
Vol 825 ◽  
pp. 540-543
Author(s):  
Mariana Moreira ◽  
Silvana de Queiroz Silva ◽  
Mônica Cristina Teixeira
Keyword(s):  
Burkholderia Cepacia ◽  
Iron Sulfide ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Dna Amplification ◽  
Bacterial Consortium ◽  
Universal Primers ◽  
Ribosomal Database Project ◽  
Sulfate Reducing ◽  
Gradient Gel Electrophoresis ◽  
Reducing Bacteria

The objective of this work was to identify one bacterial consortium adapted to the cultivation in the presence of trivalent arsenic (AsIII). Samples were cultured in flasks containing modified Postgate C liquid medium (selective for sulfate-reducing bacteria, SRB). Six different As concentrations were used: 0.5, 1.0, 2.0, 4.0, 8.0 and 16 mg l-1. The growth of sulfate reducing microorganisms was indirectly observed by the formation of an iron sulfide black precipitate and also by the Eh measures.100 ml aliquots of cultured media were centrifuged and stored at-20°C for DNA extraction by phenol/chloroform method. Universal primers 968F-GC 1392R (Bacteria domain) were used for 16S ribosomal DNA amplification. Microbial diversity was evaluated by denaturing gradient gel electrophoresis (DGGE). After DGGE analysis 7 different bands were selected, cut, sequenced and analyzed using the Ribosomal Database Project Release. Consortium microorganisms identified were: Pantoea agglomerans, Enterobacter sp, Citrobacter sp, Cupriavidusmetallidurans, Ralstonia sp, Burkholderia cepacia and Bacillus sp. Thus the microbial consortium here identified is a good candidate for bioremediation of arsenic contaminated areas and effluents.

Biofilm adaptation to sulfate reduction in anaerobic immobilized biomass reactors sujected to different COD/sulfate ratios

2006 ◽  
Vol 54 (2) ◽  
pp. 119-126 ◽  
Author(s):  
M.H.R.Z. Damianovic ◽  
I.K. Sakamoto ◽  
E. Foresti
Keyword(s):  
Molecular Biology ◽  
Sulfate Reduction ◽  
Polyurethane Foam ◽  
Horizontal Flow ◽  
Support Materials ◽  
Sulfate Reducing ◽  
Reduction Efficiency ◽  
Immobilized Biomass ◽  
High Sulfate ◽  
Reducing Bacteria

Various aspects of biofilm adaptation to sulfate reduction in horizontal-flow anaerobic immobilized biomass (HAIB) reactors subjected to increasing sulfate concentrations and different COD/sulfate ratios are presented and discussed. Four bench-scale HAIB reactors filled with vegetal carbon (R1 and R2) and polyurethane foam matrices (R3 and R4) were utilized. Influent sulfate concentrations ranging from 500 to 3000 mg/L were applied at COD/sulfate ratios ranging from 5.0 to 1.7. Reactors R1 and R4 were operated with higher sulfate loads than those applied to R2 and R3. For the same COD/sulfate ratio, the highest sulfate reduction efficiency (∼80%) was displayed by the vegetal carbon reactor (R2) subjected to low sulfate loads. According to the results of our molecular biology analyses, the different support materials provided different biomass colonization conditions. The lowest diversity of sulfate-reducing bacteria was found in the HAIB filled with polyurethane foam matrices operating with high sulfate loads.

scholarly journals Corrosion Behavior of the Ti–6Al–4V Alloy in Sulfate-Reducing Bacteria Solution

Coatings ◽  
2019 ◽  
Vol 10 (1) ◽  
pp. 24
Author(s):  
Xingwei Zheng ◽  
Xin Zhuang ◽  
Yanhua Lei ◽  
Zhenhua Chu ◽  
Jingxiang Xu ◽  
...  
Keyword(s):  
Corrosion Behavior ◽  
Pitting Corrosion ◽  
Photoelectron Spectroscopy ◽  
Sulfate Reducing Bacteria ◽  
Underlying Mechanism ◽  
Corrosion Mechanism ◽  
X Ray ◽  
Sulfate Reducing ◽  
Subsequent Period ◽  
Reducing Bacteria

The corrosion behavior of the Ti–6Al–4V alloy was investigated in a sulfate-reducing bacteria (SRB) solution. The results showed that sulfate-reducing bacteria has good affinity with the surface of the Ti–6Al–4V alloy after 5 days. A potentiodynamic polarization test demonstrated that the corrosion resistance of the Ti–6Al–4V alloy was initially improved but deteriorated quickly in the subsequent period. The corrosion mechanism of the Ti–6Al–4V alloy was revealed by analyzing its microstructure with the aid of scanning electron microscopy, X-ray photoelectron spectroscopy and X-ray fluorescence. The pitting corrosion was deemed to be a typical cause of the corrosion behavior of the alloy in the SRB solution. The underlying mechanism of the pitting corrosion was proposed for the alloy.

scholarly journals Trace Metal Imaging of Sulfate-Reducing Bacteria and Methanogenic Archaea at Single-Cell Resolution by Synchrotron X-Ray Fluorescence Imaging

2017 ◽  
Vol 35 (1) ◽  
pp. 81-89 ◽  
Author(s):  
Jennifer B. Glass ◽  
Si Chen ◽  
Katherine S. Dawson ◽  
Damian R. Horton ◽  
Stefan Vogt ◽  
...  
Keyword(s):  
Trace Metal ◽  
Single Cell ◽  
Fluorescence Imaging ◽  
Methanogenic Archaea ◽  
Sulfate Reducing Bacteria ◽  
X Ray ◽  
Sulfate Reducing ◽  
Reducing Bacteria

scholarly journals Effects of CdS NPs on Sulfate Bioreduction and Oxidative Stress to Desulfovibrio Desulfuricans

2021 ◽  
Author(s):  
Guoqing Cheng ◽  
Huili Ding ◽  
Guanglin Chen ◽  
Hongjie Shi ◽  
Xu Zhang ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Sulfate Reduction ◽  
Reduction Process ◽  
Desulfovibrio Desulfuricans ◽  
Extracellular Electron Transfer ◽  
Sulfate Reducing ◽  
Reduction Efficiency ◽  
Sulfate Reduction Process ◽  
Photosynthetic Microorganisms ◽  
Reducing Bacteria

Abstract Sulfate-containing wastewater has a serious threat to the environment and human health. Microbial technology has great potential for the treatment of sulfate-containing wastewater. It was found that nano-photocatalysts could be used as extracellular electron donors to promote the growth and metabolic activity of non-photosynthetic microorganisms. However, nano-photocatalysts could also induce oxidative stress and damage cells. In this paper, the mechanism and regulation strategy of cadmium sulfide nanoparticles（CdS NPs）on the growth of sulfate reducing bacteria and the sulfate reduction process were investigated. The results shows that the sulfate reduction efficiency could be increased by 6.43% through CdS NPs under light conditions. However, the growth of C09 was seriously inhibited by 55.00% due to the oxidative stress induced by CdS NPs on cells. The biomass and sulfate reduction efficiency could be enhanced by 6.84% and 5.85%, respectively, through external addition of humic acid (HA). At the same time, the mechanism of the CdS NPs strengthening the sulfate reduction process by sulfate bacteria was also studied. Which can provide important theoretical guidance and technical support for the development of microbial technology combined with extracellular electron transfer (EET) for the treatment of sulfate-containing wastewater.

scholarly journals Investigation of the effect of hydrocinnamic (3-phenylpropanoic) acid on the microbiological corrosion of steel

2020 ◽  
Author(s):  
А.А. Грибанькова ◽  
М.А. Агиевич ◽  
О.А. Евтуховская ◽  
В.В. Гурченко ◽  
С. Чжай ◽  
...  
Keyword(s):  
Electrochemical Impedance ◽  
Ph Value ◽  
Inhibitory Effect ◽  
Bacterial Cells ◽  
Microbiological Corrosion ◽  
Sulfate Reducing ◽  
Corrosion Of Steel ◽  
Reducing Bacteria ◽  
Number Of Bacteria ◽  
Selection Of

В основе работы лежит исследование воздействия органического соединения (ОС) – гидрокоричной (3-фенилпропановой) кислоты на бактериальные клетки СРБ (сульфатредуцирующих бактерий) являющиеся стимуляторами коррозионных процессов. Подбор условий использования и количественная оценка эффективности ингибирующего (биоцидного) действия исследуемого ОС, которое может быть применено для защиты стали от коррозии в средах, содержащих сульфатредуцирующие бактерии. В ходе исследования было изучено влияние ОС на развитие сульфатредуцирующих бактерий и определены такие параметры, как изменение значения pH и окислительно-восстановительного потенциала образцов в среде, численности бактерий и содержания биогенного сероводорода в зависимости от концентрации используемого ОС. Сделаны основные выводы о ингибирующем коррозию и биоцидном действиях испытуемого органического соединения путем оценки скорости коррозии образцов стали и защитном эффекте в зависимости от выбранных концентраций органического соединения методом гравиметрии. Дополнительная оценка ингибирующего действия была проведена методом электрохимической импедансной спектроскопии и методом снятия поляризационных кривых испытуемых образцов. The study is based on the study of the effect of an organic compound (OC) – hydrocinnamic (3-phenylpropanoic) acid on the bacterial cells of SRB (sulfate-reducing bacteria), which are stimulants of corrosion processes. Selection of conditions of use and a quantitative assessment of the effectiveness of the inhibitory (biocidal) action of the studied OC, which can be used to protect steel from corrosion in environments containing SRB. In the course of the study, the effect of OC on the development of SRB was studied and such parameters as the change in the pH value and the redox potential of the samples in the medium, the number of bacteria and the content of biogenic hydrogen sulfide depending on the concentration of the used OC were determined. The main conclusions are drawn about the inhibitory corrosion and biocidal actions of the test OC by assessing the corrosion rate of steel samples and the protective effect depending on the selected concentrations of the OC by gravimetry. An additional assessment of the inhibitory effect was carried out by the method of electrochemical impedance spectroscopy and by the method of taking the polarization curves of the test samples.

Influence of Sulfate Reducing Bacteria on Corrosion of Steel Q235 during Natural Evaporation in Soils

2012 ◽  
Vol 610-613 ◽  
pp. 243-248
Author(s):  
Xin Wang ◽  
Jin Xu ◽  
Cheng Sun
Keyword(s):  
Electrochemical Impedance Spectroscopy ◽  
Electron Probe ◽  
Electrochemical Impedance ◽  
Sulfate Reducing Bacteria ◽  
Microbiological Analysis ◽  
X Ray ◽  
Sulfate Reducing ◽  
Corrosion Rates ◽  
Corrosion Of Steel ◽  
Reducing Bacteria

Corrosion behavior of steel Q235 was investigated during natural evaporation in soils with and without sulfate reducing bacteria (SRB) by microbiological analysis, electrochemical impedance spectroscopy (EIS), energy dispersive X-ray analysis (EDXA) and electron-probe X-ray microanalysis (EPMA). The results show that during natural evaporation, oxygen content increases, amounts of SRB decrease, and the corrosion rates of steel Q235 increase with decreasing humidity of soils with and without SRB. Increments of the corrosion rates are much bigger in soils with SRB than those without SRB.

Study on Correlations between Sulfate-Reducing Bacteria and Geochemical Factors in Sediments from Baihua Lake

2012 ◽  
Vol 599 ◽  
pp. 76-80
Author(s):  
Wei Shen ◽  
Ji Wei Hu ◽  
Xian Fei Huang ◽  
Jin Mei ◽  
Li Ya Fu ◽  
...  
Keyword(s):  
Ph Value ◽  
Sampling Site ◽  
Sulfate Reducing Bacteria ◽  
Guizhou Province ◽  
Distribution Characteristics ◽  
Sediment Samples ◽  
Sulfate Reducing ◽  
Geochemical Factors ◽  
Positive Correlations ◽  
Reducing Bacteria

This paper reports a study on the distribution characteristics of sulfate-reducing bacteria (SRB) in seventeen sediment samples collected from Baihua Lake (a man-made deep plateau reservoir in Guizhou Province, China), and analyzes the correlations between the SRB content and pH value, organic matter (OM), AVS, Fe, Hg, As, Cu, Ca, Mg in the sediments. The results showed that the sediment collected from the sampling site of Yapengzhai had the highest population of SRB, and the sediment from the sampling site of Longtan SRB presented the lowest population of SRB. Moreover, there were positive correlations between SRB and OM, AVS, Fe, Hg, As, Cu, Ca, Mg whereas the negative correlations existed between the SRB content and pH value.

Sign in / Sign up

Export Citation Format

Share Document