Apoptotic cell death increases with senescence in normal human dermal fibroblast cultures

The objective of this study was to produce antibacterial poly(ε-caprolactone) (PCL)-gelatin (GEL) electrospun nanofiber mats containing clove essential oil (CLV) using glacial acetic acid (GAA) as a “benign” (non-toxic) solvent. The addition of CLV increased the fiber diameter from 241 ± 96 to 305 ± 82 nm. Aside from this, the wettability of PCL-GEL nanofiber mats was increased by the addition of CLV. Fourier-transform infrared spectroscopy (FTIR) analysis confirmed the presence of CLV, and the actual content of CLV was determined by gas chromatography–mass spectrometry (GC-MS). Our investigations showed that CLV-loaded PCL-GEL nanofiber mats did not have cytotoxic effects on normal human dermal fibroblast (NHDF) cells. On the other hand, the fibers exhibited antibacterial activity against Staphylococcus aureus and Escherichia coli. Consequently, PCL-GEL/CLV nanofiber mats are potential candidates for antibiotic-free wound healing applications.

Download Full-text

Antioxidant and Enzyme Inhibitory Activities of Areca catechu, Boesenbergia rotunda, Piper betle and Orthosiphon aristatus for Potential Skin Anti-aging Properties

Current Topics in Nutraceutical Research ◽

10.37290/ctnr2641-452x.17:229-235 ◽

2018 ◽

Vol 17 (3) ◽

pp. 229-235

Author(s):

Z.A. Mat Yasin ◽

A.S. Khazali ◽

F. Ibrahim ◽

N. Nor Rashid ◽

R. Yusof

Keyword(s):

Plant Extracts ◽

Dermal Fibroblast ◽

Radical Scavenging ◽

Human Dermal Fibroblast ◽

Piper Betle ◽

Fibroblast Cells ◽

Normal Human Dermal Fibroblast ◽

Areca Catechu ◽

Aging Properties ◽

Normal Human

Medicinal plants have been traditionally used for various applications including skin care. In this study, Areca catechu, Boesenbergia rotunda, Piper betle and Orthosiphon aristatus were tested for antioxidant and anti-aging properties. These plant extracts were subjected to 1,1-diphenyl-picrylhydrazyl (DPPH) and 2,2ʹ-azinobis(3-ethylbenzothiazoline)-6-sulphonic acid (ABTS) radical scavenging activity analyses. The extracts were then assessed for collagenase, elastase and hyaluronidase inhibition and subjected to toxicity study in normal human dermal fibroblast cells. Lastly, bioactive compounds in these plant extracts were identified using high performance liquid chromatography. We found that Piper betle and Areca catechu possessed high total flavonoid and phenolic contents. These two extracts showed the best IC50 values for DPPH and ABTS radical scavenging activities and also demonstrated the highest elastase and collagenase inhibition when compared to the other two plant extracts. These crude plants extracts were also non-toxic to normal human dermal fibroblast cells. Our HPLC results identified several potential bioactive compounds in these plant extracts that could be crucial in mediating the observed effects. Based on our results, these plant extracts especially Piper betle and Areca catechu extracts possess significant antioxidant and anti-aging properties and could be utilized in the development of safe and cost-effective anti-aging treatments.

Download Full-text

Anti-aging effects of Piper cambodianum P. Fourn. extract on normal human dermal fibroblast cells and a wound-healing model in mice

Clinical Interventions in Aging ◽

10.2147/cia.s107734 ◽

2016 ◽

Vol Volume 11 ◽

pp. 1017-1026 ◽

Cited By ~ 4

Author(s):

Jisoo Park ◽

Youngeun Hong ◽

So Hee Kwon ◽

Jongsun Park ◽

Hyunji Lee

Keyword(s):

Wound Healing ◽

Dermal Fibroblast ◽

Human Dermal Fibroblast ◽

Fibroblast Cells ◽

Aging Effects ◽

Normal Human Dermal Fibroblast ◽

Normal Human ◽

Wound Healing Model ◽

Healing Model

Download Full-text

In Vitro Bioeffects of Polyelectrolyte Multilayer Microcapsules Post-Loaded with Water-Soluble Cationic Photosensitizer

Pharmaceutics ◽

10.3390/pharmaceutics12070610 ◽

2020 ◽

Vol 12 (7) ◽

pp. 610 ◽

Cited By ~ 2

Author(s):

Alexey V. Ermakov ◽

Roman A. Verkhovskii ◽

Irina V. Babushkina ◽

Daria B. Trushina ◽

Olga A. Inozemtseva ◽

...

Keyword(s):

Targeted Delivery ◽

Dermal Fibroblast ◽

Human Dermal Fibroblast ◽

Water Soluble ◽

Zinc Phthalocyanine ◽

Bacterial Strains ◽

Normal Human Dermal Fibroblast ◽

Drug Dosing ◽

Normal Human

Microencapsulation and targeted delivery of cytotoxic and antibacterial agents of photodynamic therapy (PDT) improve the treatment outcomes for infectious diseases and cancer. In many cases, the loss of activity, poor encapsulation efficiency, and inadequate drug dosing hamper the success of this strategy. Therefore, the development of novel and reliable microencapsulated drug formulations granting high efficacy is of paramount importance. Here we report the in vitro delivery of a water-soluble cationic PDT drug, zinc phthalocyanine choline derivative (Cholosens), by biodegradable microcapsules assembled from dextran sulfate (DS) and poly-l-arginine (PArg). A photosensitizer was loaded in pre-formed [DS/PArg]4 hollow microcapsules with or without exposure to heat. Loading efficacy and drug release were quantitatively studied depending on the capsule concentration to emphasize the interactions between the DS/PArg multilayer network and Cholosens. The loading data were used to determine the dosage for heated and intact capsules to measure their PDT activity in vitro. The capsules were tested using human cervical adenocarcinoma (HeLa) and normal human dermal fibroblast (NHDF) cell lines, and two bacterial strains, Gram-positive Staphylococcus aureus and Gram-negative Escherichia coli. Our results provide compelling evidence that encapsulated forms of Cholosens are efficient as PDT drugs for both eukaryotic cells and bacteria at specified capsule-to-cell ratios.

Download Full-text