scholarly journals A Targeted Multi-omic Analysis Approach Measures Protein Expression and Low-Abundance Transcripts on the Single-Cell Level

Cell Reports ◽  
2020 ◽  
Vol 31 (1) ◽  
pp. 107499 ◽  
Author(s):  
Florian Mair ◽  
Jami R. Erickson ◽  
Valentin Voillet ◽  
Yannick Simoni ◽  
Timothy Bi ◽  
...  
Keyword(s):  
Protein Expression ◽  
Single Cell ◽  
Analysis Approach ◽  
Single Cell Level ◽  
Cell Level

scholarly journals A Targeted Multi-Omic Analysis Approach Measures Protein Expression and Low Abundance Transcripts on the Single Cell Level

2019 ◽  
Author(s):  
Florian Mair ◽  
Jami R. Erickson ◽  
Valentin Voillet ◽  
Yannick Simoni ◽  
Timothy Bi ◽  
...  
Keyword(s):  
Protein Expression ◽  
Single Cell ◽  
Analysis Approach ◽  
Single Cell Level ◽  
Cell Level

Photothermal Control of Heat-Shock Protein Expression at the Single Cell Level

Small ◽  
2018 ◽  
Vol 14 (32) ◽  
pp. 1801910 ◽  
Author(s):  
Hadrien M. L. Robert ◽  
Julien Savatier ◽  
Stéphanie Vial ◽  
Jacob Verghese ◽  
Benoit Wattellier ◽  
...  
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Protein Expression ◽  
Single Cell ◽  
Single Cell Level ◽  
Cell Level ◽  
Heat Shock Protein Expression

scholarly journals Protein Expression Analyses at the Single Cell Level

Molecules ◽  
2014 ◽  
Vol 19 (9) ◽  
pp. 13932-13947 ◽  
Author(s):  
Masae Ohno ◽  
Peter Karagiannis ◽  
Yuichi Taniguchi
Keyword(s):  
Protein Expression ◽  
Single Cell ◽  
Single Cell Level ◽  
Cell Level

Single cell-level detection and quantitation of leaky protein expression from any strongly regulated bacterial system

2015 ◽  
Vol 484 ◽  
pp. 180-182 ◽  
Author(s):  
Kanika Arora ◽  
Sachin S. Mangale ◽  
Purnananda Guptasarma
Keyword(s):  
Protein Expression ◽  
Single Cell ◽  
Single Cell Level ◽  
Cell Level ◽  
Bacterial System

scholarly journals Together is Better: mRNA Co‐Encapsulation in Lipoplexes is Required to Obtain Ratiometric Co‐Delivery and Protein Expression on the Single Cell Level

2021 ◽  
pp. 2102072
Author(s):  
Heyang Zhang ◽  
Jeroen Bussmann ◽  
Florian H. Huhnke ◽  
Joke Devoldere ◽  
An‐Katrien Minnaert ◽  
...  
Keyword(s):  
Protein Expression ◽  
Single Cell ◽  
Single Cell Level ◽  
Cell Level

scholarly journals A new triple fluorescence reporter system for discrimination of Apobec1 and Apobec3 C-to-U RNA editing activities and editing-dependent protein expression

2021 ◽  
Author(s):  
Barbara Schweissthal ◽  
Kea Brunken ◽  
Julia Brach ◽  
Leonie Emde ◽  
Florian Hetsch ◽  
...  
Keyword(s):  
Protein Expression ◽  
Single Cell ◽  
Rna Editing ◽  
Bulk Material ◽  
Splice Donor Site ◽  
Single Cell Level ◽  
Reporter System ◽  
Cell Level ◽  
Application Systems ◽  
New System

AbstractThe human body is composed of many different cell types which communicate with each other. In particular, the brain consists of billions of neurons and non-neuronal cells which are interconnected and require tight and precise regulation of cellular processes. RNA editing is a cellular process that diversifies gene function by enzymatic deamination of cytidine or adenine. This can result in changes of protein structure and function. Altered RNA editing is becoming increasingly associated with all kind of disease, but most approaches use advanced sequencing technologies to analyze bulk material. However, it is also becoming progressively evident that changes in RNA editing have to be analyzed and considered in a cell type specific way. We present here a triple fluorescence reporter system that discriminates between Apobec1- and Apobec3-dependent C-to-U RNA editing at the single cell level. In particular, the Apobec3 reporter enables C-to-U RNA editing inducible protein expression through generation of a RNA splice donor site. We used the new system here to analyze Apobec1- and Apobec3-dependent RNA editing in primary neuron culture. The results reveal a large heterogeneity of C-to-U RNA editing in neurons and glia cells, and they show that GABAergic neurons are not able to perform Apobec1-dependent RNA editing, but Apobec3-dependent editing. Altogether, the new system can be the foundation of therapeutic application systems that counteract changes in Apobec3-dependent RNA editing in disease while simultaneously monitoring Apobec1-dependent RNA editing at the single cell level.

Sign in / Sign up

Export Citation Format

Share Document