scholarly journals Real-time quantification of protein expression at the single-cell level via dynamic protein synthesis translocation reporters

2016 ◽  
Vol 7 (1) ◽  
Author(s):  
Delphine Aymoz ◽  
Victoria Wosika ◽  
Eric Durandau ◽  
Serge Pelet
Keyword(s):  
Protein Synthesis ◽  
Protein Expression ◽  
Single Cell ◽  
Real Time ◽  
Single Cell Level ◽  
Cell Level

Photothermal Control of Heat-Shock Protein Expression at the Single Cell Level

Small ◽  
2018 ◽  
Vol 14 (32) ◽  
pp. 1801910 ◽  
Author(s):  
Hadrien M. L. Robert ◽  
Julien Savatier ◽  
Stéphanie Vial ◽  
Jacob Verghese ◽  
Benoit Wattellier ◽  
...  
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Protein Expression ◽  
Single Cell ◽  
Single Cell Level ◽  
Cell Level ◽  
Heat Shock Protein Expression

scholarly journals A Targeted Multi-omic Analysis Approach Measures Protein Expression and Low-Abundance Transcripts on the Single-Cell Level

Cell Reports ◽  
2020 ◽  
Vol 31 (1) ◽  
pp. 107499 ◽  
Author(s):  
Florian Mair ◽  
Jami R. Erickson ◽  
Valentin Voillet ◽  
Yannick Simoni ◽  
Timothy Bi ◽  
...  
Keyword(s):  
Protein Expression ◽  
Single Cell ◽  
Analysis Approach ◽  
Single Cell Level ◽  
Cell Level

scholarly journals Protein Expression Analyses at the Single Cell Level

Molecules ◽  
2014 ◽  
Vol 19 (9) ◽  
pp. 13932-13947 ◽  
Author(s):  
Masae Ohno ◽  
Peter Karagiannis ◽  
Yuichi Taniguchi
Keyword(s):  
Protein Expression ◽  
Single Cell ◽  
Single Cell Level ◽  
Cell Level

Single cell-level detection and quantitation of leaky protein expression from any strongly regulated bacterial system

2015 ◽  
Vol 484 ◽  
pp. 180-182 ◽  
Author(s):  
Kanika Arora ◽  
Sachin S. Mangale ◽  
Purnananda Guptasarma
Keyword(s):  
Protein Expression ◽  
Single Cell ◽  
Single Cell Level ◽  
Cell Level ◽  
Bacterial System

Measurement of Molecular Mobility in Mammalian Cells

2004 ◽  
Author(s):  
Alptekin Aksan ◽  
Mehmet Toner
Keyword(s):  
Single Cell ◽  
Real Time ◽  
Chemical Reaction ◽  
Molecular Mobility ◽  
Mammalian Cells ◽  
Free Water ◽  
Single Cell Level ◽  
Extracellular Water ◽  
Cell Level

Preservation of mammalian cells requires establishing a reversible stasis condition by reducing the intra/extracellular molecular mobility ensuring reduced chemical reaction and deterioration rates. Molecular mobility may be reduced by various techniques. For example, in cryopreservation, mobility within and surrounding the cell is reduced through freezing the free water that constitutes 70–90% of the cell’s composition. In dried-state preservation applied successfully to preserve seeds, pharmacological materials and foodstuff (mimicking the anhydrobiosis phenomenon seen in nature), reduction in molecular mobility is established by removing intra/extracellular water. Certain carbohydrates (such as trehalose and sucrose) can be artificially uploaded into mammalian cells to replace the removed water and to form an intra/extracellular glass. In this research, a fluorescent rotor is utilized to determine the changes in intracellular molecular mobility during carbohydrate uploading of mammalian cells. It was shown that using this technique, it is feasible to make real-time mobility measurements at a single cell level.

scholarly journals Method for real-time monitoring of protein degradation at the single cell level

BioTechniques ◽  
2007 ◽  
Vol 42 (4) ◽  
pp. 446-450 ◽  
Author(s):  
Lijuan Zhang ◽  
Nadya G. Gurskaya ◽  
Ekaterina M. Merzlyak ◽  
Dmitry B. Staroverov ◽  
Nikolay N. Mudrik ◽  
...  
Keyword(s):  
Protein Degradation ◽  
Single Cell ◽  
Real Time ◽  
Single Cell Level ◽  
Real Time Monitoring ◽  
Cell Level
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