Effect of temperature and nitrogen concentration on the growth and lipid content of Nannochloropsis oculata and Chlorella vulgaris for biodiesel production

2009 ◽  
Vol 48 (6) ◽  
pp. 1146-1151 ◽  
Author(s):  
Attilio Converti ◽  
Alessandro A. Casazza ◽  
Erika Y. Ortiz ◽  
Patrizia Perego ◽  
Marco Del Borghi
Keyword(s):  
Chlorella Vulgaris ◽  
Lipid Content ◽  
Nitrogen Concentration ◽  
Nannochloropsis Oculata ◽  
Biodiesel Production ◽  
Effect Of Temperature

scholarly journals An attempt to Stimulate lipids for Biodiesel Production from locally Isolated Microalgae in Iraq

2013 ◽  
Vol 10 (1) ◽  
pp. 97-108 ◽  
Author(s):  
Baghdad Science Journal
Keyword(s):  
Chlorella Vulgaris ◽  
Lipid Content ◽  
Nitrogen Concentration ◽  
Dry Weight ◽  
Biodiesel Production ◽  
Nitrate Medium ◽  
Nitzschia Palea ◽  
High Lipid

Two locally isolated microalgae (Chlorella vulgaris Bejerinck and Nitzschia palea (Kützing) W. Smith) were used in the current study to test their ability to production biodiesel through stimulated in different nitrogen concentration treatments (0, 2, 4, 8 gl ), and effect of nitrogen concentration on the quantity of primary product (carbohydrate, protein ), also the quantity and quality of lipid. The results revealed that starvation of nitrogen led to high lipid yielding, in C. vulgaris and N. palea the lipid content increased from 6.6% to 40% and 40% to 60% of dry weight (DW) respectively.Also in C. vulgaris, the highest carbohydrate was 23% of DW from zero nitrate medium and the highest protein was 50% of DW in the treatment 8gl. While in N. palea the highest carbohydrate was 25% of DW in the treatment 4gl, and the highest protein was 15% of DW in 8gl treatment.

scholarly journals Effect of temperature and nitrogen concentration on the heterotrophic growth of Chlorella vulgaris

2016 ◽  
Vol 3 ◽  
Author(s):  
Marques Ana ◽  
Barros Ana ◽  
Correia Catarina ◽  
Silva Joana ◽  
Navalho João ◽  
...  
Keyword(s):  
Chlorella Vulgaris ◽  
Nitrogen Concentration ◽  
Effect Of Temperature ◽  
Heterotrophic Growth

Maximizing Biomass and Lipid Production in Heterotrophic Culture of Chlorella vulgaris: Techno-Economic Assessment

2019 ◽  
Vol 10 (2) ◽  
pp. 115-123 ◽  
Author(s):  
Mohammad H. Morowvat ◽  
Younes Ghasemi
Keyword(s):  
Chlorella Vulgaris ◽  
Lipid Content ◽  
Shake Flask ◽  
Biomass Productivity ◽  
Biodiesel Production ◽  
Potential Candidate ◽  
Heterotrophic Culture ◽  
Heterotrophic Cultivation ◽  
Dry Cell Weight ◽  
Autotrophic Culture

Background: Nowadays, chlorophycean microalgae have attained a broad-spectrum attention as a potential candidate for biomass and bioenergy production. Despite their appreciated benefits, one of major problems is their low biomass and lipid productivity. Here we investigated the heterotrophic culture in shake flasks and stirred tank bioreactor to improve the lipid and biomass production in a naturally isolated strain of Chlorella vulgaris. Methods: A naturally isolated C. vulgaris strain was cultivated in BG-11 medium in shake flask and bioreactor. Its biochemical composition and growth kinetic parameters were investigated. Results: The biomass productivity was improved (3.68 fold) under heterotrophic culture compared to basal autotrophic culture condition in shake flask experiment. The total lipid content increased to 44% of total Dry Cell Weight (DCW) during heterotrophic growth after 21 days. Moreover, a great Fatty Acid Methyl Esters (FAME) yield was observed under heterotrophic cultivation. Total biomass and lipid content of microalgae in bioreactor experiment increased to 4.95 and 2.18 g L-1 respectively, during 5 days of the experiment compared to its basic autotrophic culture. Conclusion: The techno-economic aspects of exploiting C. vulgaris as a biodiesel feedstock werealso evaluated. The results imply that heterotrophic cultivation could compensate the low biomass productivity in microalgae for green energy production. Ever growing rates of established patents on application of various genetic and bioengineering-based methods have made it possible to achieve higher lipid contents with reduced total costs for microalgal biodiesel production as well.

scholarly journals Nitrogen Deprivation in Fremyella diplosiphon Augments Lipid Production without Affecting Growth

Energies ◽  
2020 ◽  
Vol 13 (21) ◽  
pp. 5769
Author(s):  
Behnam Tabatabai ◽  
Afua Adusei ◽  
Alok Kumar Shrivastava ◽  
Prashant Kumar Singh ◽  
Viji Sitther
Keyword(s):  
Growth Rate ◽  
Lipid Content ◽  
Agricultural Land ◽  
Nitrogen Concentration ◽  
Lipid Production ◽  
Acid Methyl Ester ◽  
Biodiesel Production ◽  
Gravimetric Analysis ◽  
Nitrogen Deprivation ◽  
Fremyella Diplosiphon

Metabolic products such as lipids and proteins produced in cyanobacteria represent an excellent source of biomass and do not compete with agricultural land use unlike soybean and corn. Given their potential use as novel materials for biodiesel production, we aimed to explore the effect of cultivation period and nitrogen concentration on the growth rate and lipid content of Fremyella diplosiphon, a model cyanobacterium. In this study, F. diplosiphon grown in BG11/HEPES medium supplemented with 1.5 g L−1 sodium nitrate (NaNO3) for 7, 10, 15, and 20 days were compared to the untreated control in media amended with 0.25, 0.5, and 1.0 g L−1 NaNO3. Cultures were inoculated in liquid media and grown under continuous fluorescent light in an orbital incubator shaker, and extracted lipids subjected to gravimetric analysis and gas chromatography-mass spectroscopy to determine the best culture conditions for lipid production. Our results demonstrated that a reduction in nitrogen concentration had no significant effect on the growth rate across all cultivation periods; however, the accumulation of total lipid content was significantly influenced by nitrogen concentration. A maximum lipid production (40%) with no reduction in growth was observed in 10-day old cultures in a BG11/HEPES medium supplemented with 1.0 g L−1 NaNO3. Fatty acid methyl ester composition of transesterified lipids demonstrated high amounts of methyl palmitate (50–70%) followed by methyl octadecenoate (17–30%) in the accumulated lipids at all treatments. Trace quantities of methyl dodecanoate, methyl hexadecanoate, methyl octadecanoate, and methyl octadecadienoate (1–8%) were also observed in all tested samples, indicating that nitrogen deprivation in culture media increases lipid production without affecting growth.

scholarly journals Effect of Macronutrients and of Anaerobic Digestrate on The Heterotrophic Cultivation of Chlorella Vulgaris Grown With Biodiesel By-Products

2021 ◽  
Author(s):  
Nikolaos Gougoulias ◽  
George Papapolymerou ◽  
Anastasios Mpesios ◽  
Dorothea Kasiteropoulou ◽  
Maria N. Metsoviti ◽  
...  
Keyword(s):  
Organic Carbon ◽  
Protein Content ◽  
Chlorella Vulgaris ◽  
Lipid Content ◽  
Uptake Rate ◽  
Biodiesel Production ◽  
Carbon Substrate ◽  
Carbon Uptake ◽  
Atomic Nitrogen ◽  
Heterotrophic Cultivation

Abstract The aim of this work was to investigate the kinetics of the heterotrophic growth of Chlorella vulgaris as a means of producing bio-oil for biodiesel production. Glycerol was used as the sole organic carbon substrate. Anaerobic digestrate from a local plant was used to examine its effect on the kinetics and the protein and lipid content of the biomass. The effect of the initial carbon and nitrogen concentrations on the carbon uptake rate was studied independently. In one set of five experiments the organic carbon in the form of glycerol, varied from 0.27 g L-1 to 5.36 g L-1 while the concentration of atomic nitrogen was held constant and equal to 45.4 mg L-1. The Co/No ratio varied from 6 to 118.1. In a second set, also of five experiments, the organic carbon was held constant and equal to 3.3 g L-1 and atomic nitrogen varied from 22.7 mg L-1 to 450 mg L-1. The Co/No ratio varied from 7.3 to 145.4. In a third set of experiments anaerobic digestrate was added in increasing amounts into the culture media from 4% to 16%. It was found that the carbon uptake rate as well as the lipid and protein content depended on the Co/No ratio. Increasing ratios of Co/No lead to higher carbon uptake rates, higher lipid content and lower protein content. The initial nitrogen concentration was also found to affect the growth rate of C. vulgaris. The addition of anaerobic digestrate did not affect appreciably the protein and lipid content of the biomass while, addition of anaerobic digestrate up to 16% in the culture medium increased the carbon uptake rate up to about 24%.

scholarly journals Effect of Light Intensity and Quality on Growth Rate and Composition of Chlorella vulgaris

Plants ◽  
2019 ◽  
Vol 9 (1) ◽  
pp. 31 ◽  
Author(s):  
Maria N. Metsoviti ◽  
George Papapolymerou ◽  
Ioannis T. Karapanagiotidis ◽  
Nikolaos Katsoulas
Keyword(s):  
Growth Rate ◽  
Light Intensity ◽  
Chlorella Vulgaris ◽  
Lipid Content ◽  
Growth Rates ◽  
Solar Irradiance ◽  
White Led ◽  
Microalgal Biomass ◽  
Nm Range ◽  
Effect Of Light

In this research, the effect of solar irradiance on Chlorella vulgaris cultivated in open bioreactors under greenhouse conditions was investigated, as well as of ratio of light intensity in the 420–520 nm range to light in the 580–680 nm range (I420–520/I580–680) and of artificial irradiation provided by red and white LED lamps in a closed flat plate laboratory bioreactor on the growth rate and composition. The increase in solar irradiance led to faster growth rates (μexp) of C. vulgaris under both environmental conditions studied in the greenhouse (in June up to 0.33 d−1 and in September up to 0.29 d−1) and higher lipid content in microalgal biomass (in June up to 25.6% and in September up to 24.7%). In the experiments conducted in the closed bioreactor, as the ratio I420–520/I580–680 increased, the specific growth rate and the biomass, protein and lipid productivities increased as well. Additionally, the increase in light intensity with red and white LED lamps resulted in faster growth rates (the μexp increased up to 0.36 d−1) and higher lipid content (up to 22.2%), while the protein, fiber, ash and moisture content remained relatively constant. Overall, the trend in biomass, lipid, and protein productivities as a function of light intensity was similar in the two systems (greenhouse and bioreactor).

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