ATR-FTIR spectroscopy for the routine quality control of exosome isolations

UV spectroscopic method for determination of phenytoin in bulk and injection forms

10.31221/osf.io/dtrvk ◽

2019 ◽

Author(s):

Chem Int

Keyword(s):

Quality Control ◽

Spectrophotometric Method ◽

Spectroscopic Method ◽

Ich Guidelines ◽

Precision And Accuracy ◽

Routine Quality Control

Recent study was conducted to develop a simple UV spectrophotometric method to determine Phenytoin in bulk and injection form according to official requirement and validate as per ICH guidelines. λmax of Phenytoin was found 202 nm. Linearity existed perceived in the concentration assortment 2-8 μg/ml (r2 = 0.999) for the method. The method was validated pertaining to linearity, precision and accuracy studies, LOD and LOQ consistent with ICH guidelines. The existent method was establish to be simple, linear, precise, accurate as well as sensitive and can be applied for routine quality control enquiry for the analysis of Phenytoin in bulk and injection form.

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DEVELOPMENT OF HPTLC METHOD FOR SIMULTANEOUS ESTIMATION OF CIPROFLOXACIN AND ORNIDAZOLE IN COMBINED DOSAGE FORM

INDIAN DRUGS ◽

10.53879/id.50.05.p0036 ◽

2013 ◽

Vol 50 (05) ◽

pp. 36-43

Author(s):

N. R Dighade ◽

◽

M. D Shende ◽

A. V Kasture

Keyword(s):

Quality Control ◽

High Resolution ◽

Thin Layer ◽

Mobile Phase ◽

High Performance ◽

Dosage Form ◽

Simultaneous Estimation ◽

Ich Guidelines ◽

Hptlc Method ◽

Routine Quality Control

A simple and accurate high performance thin layer chromatographic (HPLTC) method has been developed and validated as per ICH guidelines for estimations of Ciprofloxacin (CP) and Ornidazole (ORN) in combined dosage form. The mobile phase was acetonitrile: toluene: water and triethylamine (5.5:1.8:1.5:1.6 V/V) was found to be best which gave high resolution with Rf 0.16 and 0.84 for ciprofloxacin and ornidazole respectively. The linearity of ciprofloxacin and ornidazole was found to be in the range of 0.4 to 0.8 µg/mL and 0.4 to 0.8 µg/mL, respectively. The coefficient of correlation (r2 ) was found to be greater than 0.989 for both the components by this method. The tablet analyses result (n = 5) were found to be > 100.84 % by HPTLC for both the components. The proposed method was found to be simple, accurate and suitable for routine quality control of marketed formulations containing these drugs.

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Análisis inmunofenotípico de muestras normales de médula ósea: aplicaciones en el control de calidad en los laboratorios de citometría

Universitas Scientiarum ◽

10.11144/javeriana.sc15-3.iaon ◽

2010 ◽

Vol 15 (3) ◽

pp. 206 ◽

Cited By ~ 1

Author(s):

Diana Carolina Roa-Higuera ◽

Susana Fiorentino-Gómez ◽

Viviana Marcela Rodríguez-Pardo ◽

Alba Myriam Campos-Arenas ◽

Elvira Antonia Infante-Acosta ◽

...

Keyword(s):

Bone Marrow ◽

Quality Control ◽

Reference Values ◽

Cell Populations ◽

Normal Bone Marrow ◽

Normal Bone ◽

Cell Subpopulations ◽

Hematologic Neoplasia ◽

Routine Quality Control ◽

Normal Leukocyte

Objective. To describe a standardized flow cytometry protocol for the relative and absolute quantification of hematopoietic cell subpopulations from normal bone marrow, and to evaluate the expression of different lineage-specific cell markers with a reactivity associated to cell differentiation to be used as part of the routine quality control in cytometry laboratories. Materials and methods. The immunophenotypical analysis of different cell subpopulations was done with samples from normal bone marrow using a panel of monoclonal and polyclonal antibodies useful in the characterization of acute leukemias with four different fluorescences, by means of a protocol that combines cell labeling of membrane and cytoplasm antigens. Expression analysis was done in terms of mean fluorescence intensity (MFI). Fluorescent beads at a known concentration were added for calculating the absolute count of cells. Results. The antibody panel used allowed the identification and quantification of different normal leukocyte subpopulations of lymphatic and myeloid origin, including CD34+ stem cells and more differentiated cell populations in the granulocytic, monocytic, and erythroid cell lines. We established reference values for cell populations and cell marker expression ranges as part of routine quality control of cytometry laboratories. Conclusion. Immunophenotypic patterns identified as well as absolute and relative reference values for the different normal leukocyte populations from bone marrow can be used by cytometry laboratories as a basis for establishing reference parameters in phenotypic analyses of hematologic neoplasia. Key words: multiparametric flow cytometry, immunophenotype, hematologic neoplasia, normal bone marrow, reference values, quality control.

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Simplified approach to confidence limited in radioimmunassay.

Clinical Chemistry ◽

10.1093/clinchem/26.6.763 ◽

1980 ◽

Vol 26 (6) ◽

pp. 763-765 ◽

Cited By ~ 12

Author(s):

R C Baxter

Keyword(s):

Quality Control ◽

Mean Value ◽

Confidence Limits ◽

Simple Method ◽

Response Variable ◽

Simplified Approach ◽

Quadratic Curve ◽

Wide Range ◽

Advanced Computing ◽

Routine Quality Control

Abstract A simple method of calculating confidence limits for radioimmunoassay data is presented. The method involves the use of the within-assay variation in dose estimate of three routine quality-control specimens, measured in repeated assays, to estimate the confidence limits for results on unknown samples. Results for control specimens are combined by calculating the unique quadratic curve fitting a graph of within-assay standard deviation vs mean value for each control. This method requires no special data accumulation or advanced computing equipment. For cortisol, lutropin, and thyroxine radioimmunoassays, confidence limits calculated in this way have been compared with those calculated from the variance of the response variable “B/B0” in repeated standard curves. Both methods agree well with actual limits observed when plasma pools containing a wide range of hormone concentrations are assayed repeatedly.

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Routine Quality Control of Clinical Nuclear Medicine Instrumentation: A Brief Review

Journal of Nuclear Medicine ◽

10.2967/jnumed.107.050203 ◽

2008 ◽

Vol 49 (7) ◽

pp. 1114-1131 ◽

Cited By ~ 78

Author(s):

P. Zanzonico

Keyword(s):

Quality Control ◽

Nuclear Medicine ◽

Nuclear Medicine Instrumentation ◽

Routine Quality Control

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Use of Tris Buffers for Quality Control of Blood pH

Clinical Chemistry ◽

10.1093/clinchem/20.10.1337 ◽

1974 ◽

Vol 20 (10) ◽

pp. 1337-1340 ◽

Cited By ~ 3

Author(s):

Jack H Ladenson ◽

Carl H Smith ◽

David N Dietzler ◽

J E Davis

Keyword(s):

Quality Control ◽

Control Program ◽

Hydrogen Ion ◽

National Bureau ◽

Quality Control Program ◽

Blood Ph ◽

Relative Standard ◽

Ion Activity ◽

Primary Standards ◽

Routine Quality Control

Abstract Tris(hydroxymethyl)aminomethane buffers have been established as sensitive and convenient indicators of the reliability of blood pH analysis. These buffers were compatible for routine use with blood-pH equipment from major manufacturers, except for two instruments. A seven-month study demonstrated the necessity of a routine quality-control program, as well as the need for systematic cleaning and maintenance of pH equipment. Tris(hydroxymethyl)aminomethane buffers were found not to be suitable as primary standards because we could not obtain the stated values with commercial pH equipment that had been standardized with phosphate buffers from the National Bureau of Standards. The relative standard deviation for measurement of hydrogen ion activity, 4.1% , indicates a need for improvement in the design and operation of equipment for the measurement of blood pH.

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