UV spectroscopic method for determination of phenytoin in bulk and injection forms

2019 ◽  
Author(s):  
Chem Int
Keyword(s):  
Quality Control ◽  
Spectrophotometric Method ◽  
Spectroscopic Method ◽  
Ich Guidelines ◽  
Precision And Accuracy ◽  
Routine Quality Control

Recent study was conducted to develop a simple UV spectrophotometric method to determine Phenytoin in bulk and injection form according to official requirement and validate as per ICH guidelines. λmax of Phenytoin was found 202 nm. Linearity existed perceived in the concentration assortment 2-8 μg/ml (r2 = 0.999) for the method. The method was validated pertaining to linearity, precision and accuracy studies, LOD and LOQ consistent with ICH guidelines. The existent method was establish to be simple, linear, precise, accurate as well as sensitive and can be applied for routine quality control enquiry for the analysis of Phenytoin in bulk and injection form.

scholarly journals Simultaneous UV Spectrophotometric Estimation of Amlodipine and Chlorthalidone in Bulk and Combined Tablet Dosage Form

2019 ◽  
pp. 468-472
Author(s):  
Sunil More ◽  
Ashpak Tamboli ◽  
Snehal Patil ◽  
Amol Vhanmane
Keyword(s):  
Spectrophotometric Method ◽  
Mobile Phase ◽  
Dosage Form ◽  
Spectroscopic Method ◽  
Simultaneous Equation ◽  
Ich Guidelines ◽  
Combined Estimation ◽  
Tablet Dosage ◽  
Different Levels

There is not a single analytical methods appeared in the literature for combined estimation of Amlodipine and Chlorthalidone in tablets dosage form. Attempts were made to develop a simple, precise and accurate Simultaneous UV spectroscopic method of Amlodipine and Chlorthalidone in bulk and Amlodac CH tablet dosage form by using simultaneous equation method. UV spectrophotometric method was developed and validated as per ICH guidelines using methanol as mobile phase. Amlodipine and Chlorthalidone individually follows the Beer-Lamberts law over concentration range 2.5-12.5μg/ml and 6.25-31.5μg/ml, Regression of coefficient was found to be r2=0.999 and r2=0.999 respectively. The percentage recovery was found in the range of 98% to 102% at three different levels. The proposed method was successfully applied for the determination of Amlodipine and Chlorthalidone in tablets dosage form as per ICH guidelines the result of the analysis were validated statistically and were found to be satisfactory.

scholarly journals DETERMINATION OF SIMULTANEOUS IRBESARTAN AND HYDROCHLOROTHIAZIDE BY ULTRAVIOLET SPECTROPHOTOMETRY WITH DUAL WAVELENGTH METHOD

1970 ◽  
Vol 7 (3) ◽  
pp. 1-4 ◽  
Author(s):  
Hafid Syahputra ◽  
M. Si. Apt Muchlisyam ◽  
M.S. Apt Masfria
Keyword(s):  
Blood Pressure ◽  
Quality Control ◽  
Spectrophotometric Method ◽  
Reduce Blood Pressure ◽  
Dual Wavelength ◽  
Good Precision ◽  
Ultraviolet Spectrophotometry ◽  
Validation Parameters ◽  
Precision And Accuracy

Objective: The present study was aimed to develop a spectrophotometric method by dual wavelength method in simultaneous analysis of irbesartan and hydrochlorothiazide on tablet preparations without separation. Irbesartan and hydrochlorothiazide are a group of anti-hypertensive drugs that are very effective and safe to use to reduce blood pressure and edema. These drugs often given in combination with these active ingredients can cause problems in quantitative analysis for the quality control of preparations. Methods: The study was carried out experimentally with a spectrophotometric method, one of which was dual wavelength method and then tested its validity based on validation parameters, namely linearity, accuracy, precision, LOD and LOQ and intraday and interday. Then, this method of irbesartan and hydrochlorothiazide in tablet preparations. Results: The results of the study were showed that the application of dual wavelength method on the concentration was carried out at λ 263.4 nm and 281 nm for irbesartan and at λ 243.4 nm and 247.6 nm for hydrochlorothiazide. The results obtained by irbesartan and hydrochlorothiazide on tablets were (108.04 ± 2.696) and (94.28 ± 4.48)% respectively, and with good precision and accuracy. Conclusions: The ultraviolet spectrophotometry method is dual wavelength method successfully applied for the determination of the concentration of irbesartan and hydrochlorothiazide in tablets.

scholarly journals Development and validation of UV spectrophotometric method for orbifloxacin assay and dissolution studies

2014 ◽  
Vol 50 (3) ◽  
pp. 457-465 ◽  
Author(s):  
Edith Cristina Laignier Cazedey ◽  
Hérida Regina Nunes Salgado
Keyword(s):  
Quality Control ◽  
Hydrochloric Acid ◽  
Spectrophotometric Method ◽  
Cost Effective ◽  
Pharmaceutical Formulation ◽  
Dissolution Studies ◽  
Linearity Range ◽  
Development And Validation ◽  
Routine Quality Control

New, simple and cost effective UV-spectrophotometric method was developed for the estimation of orbifloxacin in pharmaceutical formulation. Orbifloxacin was estimated at 290 nm in 0.5 M hydrochloric acid. Linearity range was found to be 1.0-6.0 μg mL-1. The method was tested and validated for various parameters according to main guidelines. The proposed method was successfully applied for the determination of orbifloxacin in tablets. The results demonstrated that the procedure is accurate, precise and reproducible, while being simple, economical and less time consuming. It can be suitably applied for the estimation of orbifloxacin in routine quality control and dissolution studies.

scholarly journals Quantification of Rifaximin in Tablets by Spectrophotometric Method Ecofriendly in Ultraviolet Region

Scientifica ◽  
2016 ◽  
Vol 2016 ◽  
pp. 1-9 ◽  
Author(s):  
Ana Carolina Kogawa ◽  
Hérida Regina Nunes Salgado
Keyword(s):  
Quality Control ◽  
Spectrophotometric Method ◽  
Correlation Coefficients ◽  
Environmentally Friendly ◽  
Analytical Techniques ◽  
Ultraviolet Region ◽  
Green Method ◽  
Detection And Quantification ◽  
Routine Quality Control

Rifaximin is an oral nonabsorbable antibiotic that acts locally in the gastrointestinal tract with minimal systemic adverse effects. It does not have spectrophotometric method ecofriendly in the ultraviolet region described in official compendiums and literature. The analytical techniques for determination of rifaximin reported in the literature require large amount of time to release results and are significantly onerous. Furthermore, they use toxic reagents both for the operator and environment and, therefore, cannot be considered environmentally friendly analytical techniques. The objective of this study was to develop and validate an ecofriendly spectrophotometric method in the ultraviolet region to quantify rifaximin in tablets. The method was validated, showing linearity, selectivity, precision, accuracy, and robustness. It was linear over the concentration range of 10–30 mg L−1with correlation coefficients greater than 0.9999 and limits of detection and quantification of 1.39 and 4.22 mg L−1, respectively. The validated method is useful and applied for the routine quality control of rifaximin, since it is simple with inexpensive conditions and fast in the release of results, optimizes analysts and equipment, and uses environmentally friendly solvents, being considered a green method, which does not prejudice either the operator or the environment.

Determination of Ammoniacal Nitirogen in the Presence of Urea

1980 ◽  
Vol 63 (6) ◽  
pp. 1248-1253
Author(s):  
T S Viswanathan ◽  
M Nagarajan ◽  
B Haribabu
Keyword(s):  
Quality Control ◽  
Spectrophotometric Method ◽  
Ammonium Phosphate ◽  
Ammoniacal Nitrogen ◽  
Distillation Method ◽  
Selective Electrode ◽  
Reduced Pressure ◽  
Electrode Method ◽  
Precision And Accuracy

Abstract There is no approved AOAC procedure for determining ammoniacal nitrogen in the presence of urea, which is required for the quality control of urea-ammonium phosphate-based fertilizers. A comparative study has been made of various methods available for this estimation. We found that the automated spectrophotometric method, the ion selective electrode method, and the reduced pressure distillation method all furnish results of comparable precision and accuracy. The MgO distillation method, even though not recommended for the above type of sample, still yields results of satisfactory accuracy when certain precautions are taken.

scholarly journals Simultaneous Estimation and Validation of Artemether and Lumefantrine by UV Spectrophotometry in Tablet

2021 ◽  
Vol 11 (2) ◽  
pp. 16-22
Author(s):  
Megha Mishra ◽  
Anand Mundada
Keyword(s):  
Spectrophotometric Method ◽  
Area Under Curve ◽  
Dosage Form ◽  
Spectroscopic Method ◽  
Simultaneous Estimation ◽  
Uv Spectrophotometry ◽  
Ich Guidelines ◽  
Curve Method ◽  
Tablet Dosage

A UV spectrophotometric method has been developed for the simultaneous determination of Artemether and Lumefantrine. The spectroscopic method for estimation of Artemether and Lumefantrine employed Area under curve method for analysis using Ethanol as solvent. Artemether has absorbance maxima 253.2 nm and Lumefantrine has absorbance maxima 235.2 nm and both these drugs obey Beer's law in concentration range of 4.24 -67.84 μg/ml for Artemether and 4.68 -28.08 μg/ml for Lumefantrine. The recovery studies ascertained the accuracy of the purposed method and the results were validated as per ICH guidelines. The results were found satisfactory and reproducible. The method was applied successfully for the estimation of Artemether and Lumefantrine in tablet dosage form without the interference of common excipients. Keywords: Artemether, Lumefantrine; Area under curve; Simultaneous; Estimation

scholarly journals Validation of UV Spectrophotometric and HPLC Methods for Quantitative Determination of Chlorpyrifos

2013 ◽  
Vol 21 ◽  
pp. 58-63
Author(s):  
O.A. Zalat ◽  
Mohamed A. Elsayed ◽  
M.S. Fayed ◽  
M.K. Abd El Megid
Keyword(s):  
Quality Control ◽  
Statistical Analysis ◽  
Spectrophotometric Method ◽  
Mobile Phase ◽  
Reversed Phase ◽  
Hplc Method ◽  
Routine Quality Control ◽  
Phase Column ◽  
Reversed Phase Column

A specific and sensitive HPLC and UV spectrophotometric methodwere developed for determination and analysis of chlorpyrifos. Chromatographic separation was achieved on a 150 mm x 10 mm I.D. reversed phase column Zorbax SB C-18. usingdeionizedwater: acetonitrile in the ratio of 10:90 v/v respectively as mobile phase. The effluent was monitored at 290 and 230 nm. Two sharp peaks were obtained for the solvent and chlorpyrifos at 2.7 and 3.45 min respectively. UV spectrophotometric method was performed at 290 nm using Isopropanol as the solvent. Linear range was 0.025-3500 ppm (r2 = 0.9986 ±0.0009) for HPLC method and 2.229 to 200 ppm (r2 = 0.9988) for UV spectrophotometric method. Validation guidelines and statistical analysis showed that both the methods were precise, accurate, sensitive, and can be used for the routine quality control of chlorpyrifos in waste discharges

scholarly journals UV SPECTROPHOTOMETRIC METHOD FOR QUANTITATIVE DETERMINATION OF BILASTINE USING EXPERIMENTAL DESIGN FOR ROBUSTNESS

2017 ◽  
Vol 1 (2) ◽  
pp. 38-43 ◽  
Author(s):  
Andressa Tassinari da Silva ◽  
Gabriela Rossi Brabo ◽  
Isadora Dias Marques ◽  
Lisiane Bajerski ◽  
Marcelo Donadel Malesuik ◽  
...  
Keyword(s):  
Quality Control ◽  
Liquid Chromatography ◽  
Quantitative Determination ◽  
Spectrophotometric Method ◽  
Symptomatic Treatment ◽  
Chronic Idiopathic Urticaria ◽  
H1 Receptor ◽  
H1 Receptor Antagonist ◽  
Routine Quality Control

Bilastine is a novel nonsedative H1-receptor antagonist, which may be used for the symptomatic treatment of chronic idiopathic urticaria (CU). This study describes the validation of an UV spectrophotometric method for quantitative determination of bilastine in tablets using 0.1 mol L-1 HCl as solvent. The method was specific, linear, precise, exact and robust at 210 nm, confirming that the method is fast and useful to the routine quality control of bilastine in tablets. The validate method was compared to liquid chromatography (HPLC), which was previously developed and validated to the same drug, and no significative difference between the methods using Student´s t test was found to bilastine quantitation.

scholarly journals UV Spectrophotometric Method for Determination of Cinitapride in Pure and its Solid Dosage Form

2009 ◽  
Vol 6 (s1) ◽  
pp. S21-S24 ◽  
Author(s):  
B. Thangabalan ◽  
A. Elphine Prabahar ◽  
R. Kalaichelvi ◽  
P. Vijayaraj Kumar
Keyword(s):  
Quality Control ◽  
Spectrophotometric Determination ◽  
Spectrophotometric Method ◽  
Analytical Method ◽  
Dosage Form ◽  
Spectrophotometric Assay ◽  
Solid Dosage ◽  
Quality Control Testing ◽  
Routine Quality Control

A new, rapid, precise, accurate and sensitive analytical method was developed for the UV spectrophotometric assay of cinitapride (CTP). The drug obeyed the Beer's law and showed good correlation. It showed absorption maxima at 260 nm in methanol. The linearity was observed between 5-40 µg mL-1. The results of analysis were validated by recovery studies. The recovery was more than 99%. The proposed method is the only method available for spectrophotometric determination of the drug. It is simple, precise, sensitive and reproducible and can be used for the routine quality control testing of the marketed formulations.

A Versatile Stability-indicating Liquid Chromatographic Method for the Simultaneous Determination of Atenolol, Hydrochlorothiazide and Chlorthalidone

2020 ◽  
Vol 16 (8) ◽  
pp. 1037-1051
Author(s):  
Ehab Farouk Elkady ◽  
Marwa Ahmed Fouad ◽  
Abdulgabar A. Ezzy Faquih
Keyword(s):  
Quality Control ◽  
Chromatographic Method ◽  
Degradation Products ◽  
Pharmaceutical Dosage Forms ◽  
Potassium Dihydrogen ◽  
Ich Guidelines ◽  
Liquid Chromatographic Method ◽  
Liquid Chromatographic ◽  
Potassium Dihydrogen Orthophosphate

Background: Atenolol is a selective beta 1 blocker that can be used alone or in combination with hydrochlorothiazide or with chlorthalidone for the treatment of hypertension and prevention from a heart attack. Objective: The main target of this work was to improve modern, easy, accurate and selective liquid chromatographic method (RP-HPLC) for the determination of these drugs in the presence of their degradation products. These methods can be used as analytical gadgets in quality control laboratories for a routine examination. Methods: In this method, the separation was accomplished through an Inertsil® ODS-3V C18 column (250 mm x 4.6 mm, 5 μm), the mobile phase used was 25 mM aqueous potassium dihydrogen orthophosphate solution adjusted to pH 6.8 by using 0.1M sodium hydroxide and acetonitrile (77 : 23, v/v), the flow rate used was 1 ml/min and detection was achieved at 235 nm using UV. Results: All peaks were sharp and well separated, the retention times were atenolol degradation (ATN Deg.) 2.311 min, atenolol (ATN) 2.580 min, hydrochlorothiazide degradation (HCT Deg.) 5.890 min, hydrochlorothiazide (HCT) 7.016 min, chlorthalidone degradation CTD Deg 8.018 min and chlorthalidone (CTD) 14.972 min. Linearity was obtained and the range of concentrations was 20- 160 μg/ml for atenolol, 10-80 μg/ml for hydrochlorothiazide and 10-80 μg/ml for chlorthalidone. According to ICH guidelines, method validation was accomplished, these methods include linearity, accuracy, selectivity, precision and robustness. Conclusion: The optimized method demonstrated to be specific, robust and accurate for the quality control of the cited drugs in pharmaceutical dosage forms.

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