Deconvolution of overlapped peaks based on the exponentially modified Gaussian model in comprehensive two-dimensional gas chromatography

Abstract A liquid chromatographic procedure has been developed for analysis of cyclopiazonic acid in peanuts. The chromatographic system specifies a C18 or C8 column loaded with 4-dodecyldiethylenetriamine and an aqueous mobile phase containing 4-dodecyldiethylenetriamine, zinc acetate, ammonium acetate, 2-propanol, and acetonitrile. Cyclopiazonic acid was extracted from peanuts with methanol-chloroform (20 + 80), partitioned into aqueous sodium bicarbonate, acidified, and back-extracted into dichloromethane. The limit of detection (280 nm UV) was approximately 4 ng and detector response was linear to at least 1 μg pure cyclopiazonic acid. The recovery of cyclopiazonic acid from peanuts spiked at 68.9, 210, and 955 μ/kg was 85.9% (12.86% CV), 72.9% (6.43% CV), and 81.4% (0.40% CV), respectively. Calculation of the chromatographic peak parameters based on the exponentially modified Gaussian model indicated that the C18 column produced less peak skewing than did the C8 column.

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Faculty Opinions recommendation of Application of headspace solid-phase microextraction (HS-SPME) and comprehensive two-dimensional gas chromatography (GC x GC) for the chemical profiling of volatile oils in complex herbal mixtures.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1024700.292806 ◽

2005 ◽

Author(s):

Oliver Fiehn

Keyword(s):

Gas Chromatography ◽

Solid Phase Microextraction ◽

Solid Phase ◽

Two Dimensional ◽

Volatile Oils ◽

Headspace Solid Phase Microextraction ◽

Chemical Profiling ◽

Gc X Gc ◽

Herbal Mixtures

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Characterization of compounds in crude oils by gas purge micro-syringe extraction coupled to comprehensive two-dimensional gas chromatography

Chinese Journal of Chromatography ◽

10.3724/sp.j.1123.2014.07017 ◽

2014 ◽

Vol 32 (10) ◽

pp. 1144

Author(s):

Ting TONG ◽

Wanfeng ZHANG ◽

Donghao LI ◽

Jinhua ZHAO ◽

Zhenyang CHANG ◽

...

Keyword(s):

Gas Chromatography ◽

Crude Oils ◽

Two Dimensional ◽

Gas Purge

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Analysis of volatile oils of Ligusticum chuanxiong Hort. from different geographical origins by comprehensive two-dimensional gas chromatography/time-of-flight mass spectrometry

Chinese Journal of Chromatography ◽

10.3724/sp.j.1123.2010.00329 ◽

2010 ◽

Vol 28 (4) ◽

pp. 329-335 ◽

Cited By ~ 2

Author(s):

Nan WANG ◽

Yi ZHANG ◽

Xiang LI ◽

Yingpeng TONG ◽

Hongwei KONG ◽

...

Keyword(s):

Mass Spectrometry ◽

Gas Chromatography ◽

Time Of Flight ◽

Two Dimensional ◽

Volatile Oils ◽

Ligusticum Chuanxiong ◽

Flight Mass Spectrometry

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New Methods for Analyzing Water Pollutants

Water Science & Technology ◽

10.2166/wst.1982.0087 ◽

1982 ◽

Vol 14 (4-5) ◽

pp. 59-71 ◽

Cited By ~ 6

Author(s):

L H Keith ◽

R C Hall ◽

R C Hanisch ◽

R G Landolt ◽

J E Henderson

Keyword(s):

Amino Acids ◽

Gas Chromatography ◽

Two Dimensional ◽

Gas Chromatograph ◽

New Class ◽

New Methods ◽

System A ◽

Drinking Waters ◽

Computer Controlled ◽

Nitrogen Containing Compounds

Two new methods have been developed to analyze for organic pollutants in water. The first, two-dimensional gas chromatography, using post detector peak recycling (PDPR), involves the use of a computer-controlled gas Chromatograph to selectively trap compounds of interest and rechromatograph them on a second column, recycling them through the same detector again. The second employs a new detector system, a thermally modulated electron capture detector (TMECD). Both methods were used to demonstrate their utility by applying them to the analysis of a new class of potentially ubiquitous anthropoaqueous pollutants in drinking waters- -haloacetonitriles. These newly identified compounds are produced from certain amino acids and other nitrogen-containing compounds reacting with chlorine during the disinfection stage of treatment.

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