Application of high-performance liquid chromatography–electrospray ionization time-of-flight mass spectrometry for analysis and quality control of Radix Astragali and its preparations

Abstract The dried root of Asparagus cochinchinensis (RAC) has been used as an important traditional Chinese medicine for a long time in China. Steroidal saponins (SSs) are considered to be the main active ingredients of this herb. However, the isolation and structural determination of SSs from RAC are time-consuming and laborious. For this reason, the development of new methods for the separation and characterization of SSs is highly desirable. In this study, a new high-performance liquid chromatography coupled to electrospray ionization and quadrupole time-of-flight mass spectrometry (HPLC-ESI-QTOF-MS/MS) method with precursor ions and the corresponding fragment ions was developed for the identification of SSs in RAC. Finally, 30 SSs have been detected and identified, including 17 potential new compounds. This is the first systematic study of SSs in RAC by HPLC-ESI-QTOF-MS/MS method.

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Rapid analysis of short- and medium-chain chlorinated paraffins in wine by dispersive liquid–liquid micro-extraction coupled with high performance liquid chromatography-electrospray ionization quadrupole time-of-flight mass spectrometry

Food Chemistry ◽

10.1016/j.foodchem.2020.126583 ◽

2020 ◽

Vol 319 ◽

pp. 126583

Author(s):

Xi Zhou ◽

Huiqin Wu ◽

Xiaolan Huang ◽

Fang Hang ◽

Huitai Luo

Keyword(s):

Mass Spectrometry ◽

High Performance Liquid Chromatography ◽

Liquid Chromatography ◽

Electrospray Ionization ◽

High Performance ◽

Time Of Flight ◽

Rapid Analysis ◽

Medium Chain ◽

Flight Mass Spectrometry ◽

Chlorinated Paraffins

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Chemical profiling of Dioscorea septemloba by High Performance Liquid Chromatography Coupled to Electrospray Ionization and Quadrupole Time-of-Flight Mass Spectrometry

Journal of Chromatographic Science ◽

10.1093/chromsci/bmaa039 ◽

2020 ◽

Vol 58 (8) ◽

pp. 709-717

Author(s):

Yun Ling ◽

Lu Chen ◽

Dan Wang ◽

Xin Xiao ◽

Wenjing Xu ◽

...

Keyword(s):

Mass Spectrometry ◽

High Performance Liquid Chromatography ◽

Liquid Chromatography ◽

Electrospray Ionization ◽

High Performance ◽

Time Of Flight ◽

Negative Ion ◽

Chemical Compositions ◽

Flight Mass Spectrometry ◽

Promising Source

Abstract The rhizomes of Dioscorea septemloba (DS) is one of traditional Chinese medicine, which can be used for treatments of urethral and renal infections, as well as rheumatism. Nevertheless, detailed information on DS chemical compositions is still scarce. Therefore, the present work is aimed at investigating the phytochemical components of DS using high performance liquid chromatography coupled to electrospray ionization and quadrupole time-of-flight mass spectrometry in negative ion mode. The proposed method provided tentative identification of 32 components, 6 of which were determined to be new. The present study highlighted the importance of DS as a promising source of medicinal ingredients and provides valuable information on the new potential components in DS.

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