Two-Dimensional High-Performance Liquid Chromatography Using Monodisperse Polymer Beads Containing Segregated Chemistries Prepared by Pore Size Specific Functionalization. Single-Column Combinations of Size Exclusion or Ion Exchange with Reversed-Phase Chromatography

1994 ◽  
Vol 66 (23) ◽  
pp. 4308-4315 ◽  
Author(s):  
Vladimir. Smigol ◽  
Frantisek. Svec ◽  
Jean M. J. Frechet
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Ion Exchange ◽  
Pore Size ◽  
High Performance ◽  
Reversed Phase ◽  
Size Exclusion ◽  
Two Dimensional ◽  
Reversed Phase Chromatography ◽  
Single Column

Separation of Liquiritin by Two-Dimensional Liquid Chromatography

2012 ◽  
Vol 455-456 ◽  
pp. 1232-1238 ◽  
Author(s):  
Jing Xiang Cong ◽  
Shao Yan Wang ◽  
Hong Gao
Keyword(s):  
Liquid Chromatography ◽  
Reversed Phase ◽  
Normal Phase ◽  
Size Exclusion ◽  
Two Dimensional ◽  
Target Compound ◽  
Reversed Phase Chromatography ◽  
Complex Samples ◽  
Exclusion Chromatography ◽  
Licorice Extract

Two-dimensional liquid chromatography (2DLC) is an important technology for the separation and analysis of complex samples. Liquiritin, an important active component in licorice, was chosen as the target compound and it was separated by three kinds of off-line 2DLC, i.e. size exclusion chromatography × reversed phase chromatography, normal phase × reversed phase chromatography and reversed phase chromatography × reversed phase chromatography (SEC×RP, NP×RP and RP×RP). The chromatographic conditions were selected and the 2D systems were combined. The results show that it is feasible to separate Liquiritin from licorice extract using 2DLC. Among the 2D modes mentioned above, the highest purity of Liquiritin was obtained in the RP×RP mode, and the concentration of Liquiritin was increased most significantly in the NP×RP mode.

scholarly journals Separation of New Coumarin Glycosides from Toddalia asiatica Using Offline Two-Dimensional High-Performance Liquid Chromatography

Plants ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 428 ◽  
Author(s):  
Yan Li ◽  
Shi-Wei Sun ◽  
Xiao-Yi Zhang ◽  
Yang Liu ◽  
Xiao-Hong Liu ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Purity ◽  
High Performance ◽  
Stationary Phases ◽  
Reversed Phase ◽  
Two Dimensional ◽  
Toddalia Asiatica ◽  
Phase Column ◽  
Reversed Phase Column

Coumarins and flavonoids are the major constituents of Toddalia asiatica. The separation and purification of ingredients from T. asiatica is an important procedure to acquire high-purity compounds for subsequent pharmacological investigation to discover leading compounds. In the present work, an offline two-dimensional high-performance liquid chromatography (HPLC) method was successfully established for the separation of high-purity glycosides from T. asiatica. Based on the separation results obtained with two different chromatographic stationary phases, a phenyl-bonded silica-based reversed-phase column was employed as the first HPLC preparation, and three fractions were obtained from the sample. Then, the fractions were isolated and purified on an octadecyl-bonded silica-based reversed-phase column to obtain high-purity compounds in the second HPLC separation. As a result, three coumarin glycosides, including two undescribed and one known, along with one known flavonoid glycoside with more than 98% purity were isolated from the sample. The structures of the isolated compounds were elucidated on the basis of extensive spectroscopic evidence derived from optical rotation, mass spectrometry, and nuclear magnetic resonance experiments. Two-dimensional HPLC with different stationary phases has the potential to be an efficient method for the separation of high-purity compounds from T. asiatica.

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