A rapid, micro-scale preliminary screening method for active components in Galangal with protective effect against hydrogen peroxide induced cell apoptosis through “thin layer chromatography” and “tetrazolium-based colorimetric assay” array correspondence

2015 ◽  
Vol 1395 ◽  
pp. 167-172
Author(s):  
Yuan Cheng ◽  
Yuanting Li ◽  
Jin Li ◽  
Yifeng Deng
Keyword(s):  
Hydrogen Peroxide ◽  
Thin Layer ◽  
Protective Effect ◽  
Cell Apoptosis ◽  
Colorimetric Assay ◽  
Screening Method ◽  
Active Components ◽  
Preliminary Screening ◽  
Micro Scale ◽  
Layer Chromatography

Screening Method for the Detection of Aflatoxins, Ochratoxin, Patulin, Sterigmatocystin, and Zearalenone in Cereals

1977 ◽  
Vol 60 (6) ◽  
pp. 1369-1371 ◽  
Author(s):  
B G Egon Josefsson ◽  
Tord E Möller
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Ochratoxin A ◽  
Screening Method ◽  
Gel Filtration ◽  
Limits Of Detection ◽  
Layer Chromatography

Abstract A screening method has been developed for the detection of aflatoxins, ochratoxin A, patulin, sterigmatocystin, and zearalenone in cereals. After extraction, the sample is cleaned up by gel filtration. The mycotoxins are separated by thin layer chromatography. The limits of detection are about 5 μg aflatoxins, 10 ochratoxin A, 50 μg patulin, 10 μg sterigmatocystin, and 35 μg zearalenone/kg.

Thin Layer Chromatographic Screening Method for Sulfadiazine Residues in Calf Tissues, Plasma, and Urine

1978 ◽  
Vol 61 (3) ◽  
pp. 545-549
Author(s):  
Joseph L Woolley ◽  
Oliver Murch ◽  
Carl W Sigel
Keyword(s):  
Thin Layer ◽  
Screening Method ◽  
Target Tissue ◽  
Highly Sensitive ◽  
Tissue Homogenates ◽  
Specific Tissue ◽  
Sulfonamide Residues ◽  
Kidney Liver ◽  
Layer Chromatography ◽  
Endogenous Substances

Abstract Because of the lack of specificity of the Bratton-Marshall procedure for assaying sulfonamides, a sensitive, specific tissue residue assay for sulfadiazine (SDZ) was developed. The methodology has been extended to provide a highly sensitive screen for sulfonamide residues, which employs 2-dimensional thin layer chromatography in conjunction with fluorescamine derivatization. The procedure described, which has been developed for SDZ in calf tissues, involves direct ethyl acetate extraction of tissue homogenates. Following evaporation of the organic phase, a portion of the residue is spotted on a 20x20 cm silica gel 60 plate, which is then developed in 2 dimensions with solvent systems devised to separate SDZ from endogenous substances as well as from 12 other sulfonamides that might be present in calf tissues. The presence of SDZ at a concentration of 0.1 ppm or its absence is easily demonstrated in calf kidney, liver, muscle, plasma, and urine. The basic method can be modified for a particular sulfonamide in a target tissue and can be used as a quantitative assay for sulfonamide residues.

scholarly journals Yeast-Based Fluorescent Sensors for the Simultaneous Detection of Estrogenic and Androgenic Compounds, Coupled with High-Performance Thin Layer Chromatography

Biosensors ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 169
Author(s):  
Liat Moscovici ◽  
Carolin Riegraf ◽  
Nidaa Abu-Rmailah ◽  
Hadas Atias ◽  
Dror Shakibai ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Simultaneous Detection ◽  
Endocrine Disrupting Compounds ◽  
Pollution Monitoring ◽  
Analytical Sensitivity ◽  
Active Components ◽  
Layer Chromatography

The persistence of endocrine disrupting compounds (EDCs) throughout wastewater treatment processes poses a significant health threat to humans and to the environment. The analysis of EDCs in wastewater remains a challenge for several reasons, including (a) the multitude of bioactive but partially unknown compounds, (b) the complexity of the wastewater matrix, and (c) the required analytical sensitivity. By coupling biological assays with high-performance thin-layer chromatography (HPTLC), different samples can be screened simultaneously, highlighting their active components; these may then be identified by chemical analysis. To allow the multiparallel detection of diverse endocrine disruption activities, we have constructed Saccharomyces cerevisiae-based bioreporter strains, responding to compounds with either estrogenic or androgenic activity, by the expression of green (EGFP), red (mRuby), or blue (mTagBFP2) fluorescent proteins. We demonstrate the analytical potential inherent in combining chromatographic compound separation with a direct fluorescent signal detection of EDC activities. The applicability of the system is further demonstrated by separating influent samples of wastewater treatment plants, and simultaneously quantifying estrogenic and androgenic activities of their components. The combination of a chemical separation technique with an optical yeast-based bioassay presents a potentially valuable addition to our arsenal of environmental pollution monitoring tools.

scholarly journals Thin-Layer Chromatographic Determination of Monensin in Feeds: Screening Method

1998 ◽  
Vol 81 (4) ◽  
pp. 844-847 ◽  
Author(s):  
Wynne W Landgraf ◽  
P Frank Ross
Keyword(s):  
Solid Phase Extraction ◽  
Thin Layer ◽  
Solid Phase ◽  
Screening Method ◽  
Chromatographic Determination ◽  
Phase Extraction ◽  
Color Development ◽  
Layer Chromatography ◽  
Positive Results

Abstract Monensin is extracted from feed with methanol and purified by solvent-partitioning solid-phase extraction. After solvent reduction, monensin is separated by thin-layer chromatography on silica gel and visualized by color development with vanillin. No false-positive results were obtained in validation studies by submitting or peer laboratories when blank samples were analyzed. Three of 20 samples spiked with 5 ppm monensin were reported as containing no monensin. All samples spiked with 10 ppm monensin were reported positive for monensin.

High efficiency screening of nine lipid-lowering adulterants in herbal dietary supplements using thin layer chromatography coupled with surface enhanced Raman spectroscopy

2017 ◽  
Vol 9 (10) ◽  
pp. 1595-1602 ◽  
Author(s):  
Qingxia Zhu ◽  
Mengyun Chen ◽  
Lu Han ◽  
Yongfang Yuan ◽  
Feng Lu
Keyword(s):  
Raman Spectroscopy ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Efficiency ◽  
Screening Method ◽  
Surface Enhanced Raman Spectroscopy ◽  
Lipid Lowering ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Layer Chromatography

A high efficiency screening method was developed to analyze lipid-lowering adulterants in complicated HDS systems.

Simple Colorimetric Estimation of Cyclopiazonic Acid in Contaminated Food and Feeds

1984 ◽  
Vol 67 (1) ◽  
pp. 38-40
Author(s):  
A Rathinavelu ◽  
Edayathimangalam Rajabhavani B Shanmugasundaram
Keyword(s):  
Thin Layer ◽  
Quantitative Determination ◽  
Thin Layer Chromatography ◽  
Minimal Medium ◽  
Colorimetric Assay ◽  
Cyclopiazonic Acid ◽  
Assay Procedure ◽  
Contaminated Food ◽  
Layer Chromatography

Abstract A method has been developed for quantitative determination of cyclopiazonic acid, a mycotoxin produced by a common food contaminant, Penicillium cyclopium. The organism was grown successively in synthetic minimal medium, rice, corn, and wheat for 15 days. The toxin was extracted with chloroform followed by separation by thin layer chromatography. A colorimetric assay procedure has been successfully developed for the analysis of cyclopiazonic acid present in infected rice, corn, and wheat. The sensitivity of the method was tested by using recovery experiments.

Rapid Screening Method for Deoxynivalenol in Agricultural Commodities by Fluorescent Minicolumn

1990 ◽  
Vol 73 (2) ◽  
pp. 266-270
Author(s):  
William C Gordon ◽  
Linda J Gordon
Keyword(s):  
Gas Chromatography ◽  
Thin Layer ◽  
Selective Adsorption ◽  
Screening Method ◽  
Rapid Screening ◽  
Blue Fluorescence ◽  
False Negatives ◽  
Rapid Screening Method ◽  
Gas Chromatography Mass Spectroscopy ◽  
Layer Chromatography

Abstract A rapid screening procedure based on the selective adsorption of deoxynivalenol (DON) from extracts of wheat and corn has been developed. DON is extracted from the sample with acetonitrile-water (85 + 15) and partially purified on a preparative minicolumn. Solvent is evaporated and the residue is dissolved in toluene-acetone (95 + 5) and chromatographed on a novel detector minicolumn which selectively adsorbs DON. A blue fluorescence is produced when the column is heated 5 min at 100°C. The procedure is capable of detecting DON at ≥ 500 ng/g. Forty-three wheat samples, contaminated with DON at 60-6300 ng/g, were assayed by gas chromatography-mass spectroscopy (GC-MS) of the heptafluorobutyryl derivative of DON and by the selective adsorption procedure. Comparison of results showed 9 1% agreement between data from the 2 methods. Selective adsorption assays were positive for all samples that were ≥ 500 ng/g by GC-MS (no false negatives) and were negative for 85 % of samples < 500 ng/g (4/27 false positives). These four samples contained > 200 ng/g by GC-MS. Samples of wheat (64), corn (23), soybeans (8), and sorghum (6) were extracted and extracts were assayed by thin-layer chromatography and the selective adsorption procedure. Selective adsorption assays agreed with TLC results.

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