Using the column wall itself as resistive heater for fast temperature gradients in liquid chromatography

2015 ◽  
Vol 1420 ◽  
pp. 129-134 ◽  
Author(s):  
Ruben De Pauw ◽  
Matthias Pursch ◽  
Gert Desmet
Keyword(s):  
Liquid Chromatography ◽  
Temperature Gradients ◽  
Column Wall ◽  
Resistive Heater

scholarly journals Theoretical Analysis of Forced Segmented Temperature Gradients in Liquid Chromatography

Processes ◽  
2019 ◽  
Vol 7 (11) ◽  
pp. 846
Author(s):  
Adnan Hayat ◽  
Xinghai An ◽  
Shamsul Qamar ◽  
Gerald Warnecke ◽  
Andreas Seidel-Morgenstern
Keyword(s):  
Liquid Chromatography ◽  
Mobile Phase ◽  
Method Of Characteristics ◽  
Solute Concentration ◽  
Separation Process ◽  
Temperature Gradients ◽  
Performance Criteria ◽  
Concentration Profiles ◽  
Column Wall ◽  
Fixed Reference

An equilibrium model is applied to study the effect of forced temperature gradients introduced through heat exchange via specific segments of the wall of a chromatographic column operating with a liquid mobile phase. For illustration of the principle, the column is divided into two segments in such a manner that the first segment is kept at a fixed reference temperature, while the temperature of the second segment can be changed stepwise through fixed heating or cooling over the column wall to modulate the migration speeds of the solute concentration profiles. The method of characteristics is used to obtain the solution trajectories analytically. It is demonstrated that appropriate heating or cooling in the second segment can accelerate or decelerate the specific concentration profiles in order to improve certain performance criteria. The results obtained verify that the proposed analysis is well suited to evaluate the application of forced segmented temperature gradients. The suggested gradient procedure provides the potential to reduce the cycle time and, thus, improving the production rate of the chromatographic separation process compared to conventional isothermal (isocratic) operation.

Implementations of temperature gradients in temperature-responsive liquid chromatography

2021 ◽  
pp. 462425
Author(s):  
Mathijs Baert ◽  
Kristina Wicht ◽  
Ali Moussa ◽  
Gert Desmet ◽  
Ken Broeckhoven ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Temperature Gradients ◽  
Temperature Responsive

Advanced Liquid Chromatography of Polyolefins Using Simultaneous Solvent and Temperature Gradients

2020 ◽  
Vol 92 (10) ◽  
pp. 7325-7333 ◽  
Author(s):  
Anthony Ndiripo ◽  
Andreas Albrecht ◽  
Harald Pasch
Keyword(s):  
Liquid Chromatography ◽  
Temperature Gradients

Resolution of alkenylacylglycerol moieties of natural glycerophospholipids by gas–liquid chromatography on polar capillary columns

1984 ◽  
Vol 62 (6) ◽  
pp. 352-362 ◽  
Author(s):  
J. J. Myher ◽  
A. Kuksis
Keyword(s):  
Liquid Chromatography ◽  
Carbon Number ◽  
Glass Capillary Column ◽  
Gas Liquid Chromatography ◽  
Glass Capillary ◽  
Practical Procedure ◽  
Flame Ionization ◽  
Column Wall ◽  
Layer Chromatography

A sensitive and practical procedure has been developed for the determination of the sn-1-alk-1-enyl-2-acylglycerol moieties of natural glycerophospholipids using capillary gas–liquid chromatography with flame ionization detection. The method gives excellent resolution and quantitative estimates for molecular species according to carbon number and degree of unsaturation. The diradylglycerols are released from the glycerophospholipids by hydrolysis with phospholipase C and the alk-1-enylacylglycerols are resolved by thin-layer chromatography. Either the trimethylsilyl or the tertiary-butyldimethylsilyl ethers are then resolved at 250 °C by gas–liquid chromatography on a glass capillary column wall-coated with a polar cyanopropylsiloxane polymer using hydrogen as the carrier gas. The method has been applied to the analysis of the alk-1-enylacyl- and diacyl-glycerol moieties of the phosphatidylethanolamines of rat heart and kidney.

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