Control of retention mechanisms on an octadecyl-bonded silica column using ionic liquid-based mobile phase in analysis of cytostatic drugs by liquid chromatography

2021 ◽  
pp. 462257
Author(s):  
Natalia Treder ◽  
Ilona Olędzka ◽  
Anna Roszkowska ◽  
Tomasz Bączek ◽  
Alina Plenis
Keyword(s):  
Ionic Liquid ◽  
Liquid Chromatography ◽  
Mobile Phase ◽  
Cytostatic Drugs ◽  
Retention Mechanisms ◽  
Silica Column

scholarly journals A Liquid Chromatography Procedure for Analysis of Nicotine on Cellulose Acetate Filters

1985 ◽  
Vol 13 (1) ◽  
pp. 11-16 ◽  
Author(s):  
CR Green ◽  
FW Conrad ◽  
JKA Bridle ◽  
MF Borgerding
Keyword(s):  
Liquid Chromatography ◽  
Cellulose Acetate ◽  
Phosphate Buffer ◽  
Mobile Phase ◽  
Steam Distillation ◽  
Ultra Violet ◽  
Silica Column ◽  
Mobile Phases ◽  
Second Procedure ◽  
Filter Analysis

AbstractA procedure has been developed for filter analysis whereby the cellulose acetate is dissolved in acetonitrile to release any trapped nicotine. Dissolving the filter eliminates time consuming steam distillation or solvent extraction steps and assures that the recovery of nicotine is complete. After the filter is dissolved, the cellulose acetate is precipitated by addition of an amine-phosphate buffer and an aliquot of the filtered solution is analysed by high-pressure liquid chromatography (HPLC). Two methods of HPLC analysis are described. In both cases the separation is achieved on a cyano-bonded silica column and detection is by ultra-violet absorption at 254 nm. Different mobile phases are used in the two methods. In the first procedure, a diethylamine-phosphate buffer at pH 7.56 is used while in the second procedure, a dimethylamine-phosphate buffer at pH 3.00 is used. Analytical results are equivalent for both chromatographic methods, but the second procedure may offer extended analytical column life. Results of a study relating the structure of the amine in the mobile phase to nicotine retention are presented. The amount of nicotine trapped on cellulose acetate filters during smoking was determined with increasing intervals between smoking and analysis. These results demonstrate that nicotine is stable on filters and previous problems of analysis were caused by difficulty in removal from aged filters.

Hydrophilic Interaction Liquid Chromatography and Per Aqueous Liquid Chromatography in Fungicides Analysis

2012 ◽  
Vol 95 (5) ◽  
pp. 1362-1370 ◽  
Author(s):  
Sylwia Noga ◽  
Attila Felinger ◽  
Bogusław Buszewski
Keyword(s):  
Liquid Chromatography ◽  
Organic Solvent ◽  
Mobile Phase ◽  
Retention Mechanism ◽  
Operating Conditions ◽  
Biologically Active ◽  
Hydrophilic Interaction Liquid Chromatography ◽  
Hydrophilic Interaction ◽  
Active Compounds ◽  
Retention Mechanisms

Abstract The goal of the study was to investigate the retention mechanism of selected fungicides in hydrophilic interaction liquid chromatography (HILIC) and per aqueous liquid chromatography (PALC). Chromatographic measurements were made on four physicochemically diversified HILIC columns, which were evaluated for the analysis of nine biologically active compounds, such as strobilurins and triazoles. The effects of the operating conditions on separations were investigated, including the concentration of the organic solvent in the aqueous-organic (acetonitrile) mobile phase. The results were compared, and it was shown that two different retention mechanisms dominate in PALC at low acetonitrile concentrations and in HILIC at high acetonitrile concentrations.

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