scholarly journals Comparison of cell stabilizing blood collection tubes for circulating plasma tumor DNA

2015 ◽  
Vol 48 (15) ◽  
pp. 993-998 ◽  
Author(s):  
Patricia Valda Toro ◽  
Bracha Erlanger ◽  
Julia A. Beaver ◽  
Rory L. Cochran ◽  
Dustin A. VanDenBerg ◽  
...  
Keyword(s):  
Blood Collection ◽  
Blood Collection Tubes ◽  
Tumor Dna ◽  
Plasma Tumor Dna

Comparative analysis of circulating tumor DNA stability In K3EDTA, Streck, and CellSave blood collection tubes

2016 ◽  
Vol 49 (18) ◽  
pp. 1354-1360 ◽  
Author(s):  
Qing Kang ◽  
N. Lynn Henry ◽  
Costanza Paoletti ◽  
Hui Jiang ◽  
Pankaj Vats ◽  
...  
Keyword(s):  
Comparative Analysis ◽  
Circulating Tumor Dna ◽  
Blood Collection ◽  
Dna Stability ◽  
Blood Collection Tubes ◽  
Tumor Dna

scholarly journals Circulating-tumor DNA from Two Types of Blood Collection Tubes for Monitoring Tumor Burden Validated with Esophageal Squamous Cell Carcinoma Patients

2020 ◽  
Author(s):  
Fumitaka Endo ◽  
Takeshi Iwaya ◽  
Yasushi Sasaki ◽  
Takehiro Chiba ◽  
Mizunori Yaegashi ◽  
...  
Keyword(s):  
Esophageal Cancer ◽  
Mononuclear Cell ◽  
Circulating Tumor Dna ◽  
Tumor Burden ◽  
Blood Collection ◽  
Plasma Dna ◽  
Peripheral Blood Mononuclear ◽  
Blood Collection Tubes ◽  
Tumor Dna

Objective: To evaluate long-term whole blood (WB) storage at room temperature (RT), plasma and peripheral blood mononuclear cell (PBMC) DNA was collected simultaneously using BD Vacutainer® CPT™ Mononuclear Cell Preparation Tubes (CPT) and Streck Cell-Free DNA Blood Collection Tubes (BCT®). Methods: Plasma DNA was isolated from both types of tubes at various time points at RT. DNA from PBMCs was extracted using CPT from WB stored in BCT. The extracted DNA was used to monitor esophageal cancer treatment. Results: BCT maintained steady levels of plasma DNA for up to nine days. After transfer of BCT-stored WB to CPT, PBMC-DNA were yielded with suitable quality for up to seven days. ctDNA from esophageal cancer patients carrying TP53 mutations reflected treatment efficacy. Conclusion: BCT/CPT combinatory procedure allows storage of blood samples for up to seven days at RT for valid clinical assays using ctDNA.

scholarly journals Comparison of Blood Collection Tubes from Three Different Manufacturers for the Collection of Cell-Free DNA for Liquid Biopsy Mutation Testing

2017 ◽  
Vol 19 (5) ◽  
pp. 801-804 ◽  
Author(s):  
Christina Alidousty ◽  
Danielle Brandes ◽  
Carina Heydt ◽  
Svenja Wagener ◽  
Maike Wittersheim ◽  
...  
Keyword(s):  
Liquid Biopsy ◽  
Mutation Testing ◽  
Blood Collection ◽  
Cell Free Dna ◽  
Free Dna ◽  
Blood Collection Tubes

scholarly journals Avoidable Blood Loss in Critical Care and Patient Blood Management: Scoping Review of Diagnostic Blood Loss

2022 ◽  
Vol 11 (2) ◽  
pp. 320
Author(s):  
Philipp Helmer ◽  
Sebastian Hottenrott ◽  
Andreas Steinisch ◽  
Daniel Röder ◽  
Jörg Schubert ◽  
...  
Keyword(s):  
Intensive Care ◽  
Blood Loss ◽  
Blood Volume ◽  
Scoping Review ◽  
Relevant Information ◽  
Blood Collection ◽  
Patient Blood Management ◽  
Blood Management ◽  
Intensive Care Patients ◽  
Blood Collection Tubes

Background: Anemia remains one of the most common comorbidities in intensive care patients worldwide. The cause of anemia is often multifactorial and triggered by underlying disease, comorbidities, and iatrogenic factors, such as diagnostic phlebotomies. As anemia is associated with a worse outcome, especially in intensive care patients, unnecessary iatrogenic blood loss must be avoided. Therefore, this scoping review addresses the amount of blood loss during routine phlebotomies in adult (>17 years) intensive care patients and whether there are factors that need to be improved in terms of patient blood management (PBM). Methods: A systematic search of the Medline Database via PubMed was conducted according to PRISMA guidelines. The reported daily blood volume for diagnostics and other relevant information from eligible studies were charted. Results: A total of 2167 studies were identified in our search, of which 38 studies met the inclusion criteria (9 interventional studies and 29 observational studies). The majority of the studies were conducted in the US (37%) and Canada (13%). An increasing interest to reduce iatrogenic blood loss has been observed since 2015. Phlebotomized blood volume per patient per day was up to 377 mL. All interventional trials showed that the use of pediatric-sized blood collection tubes can significantly reduce the daily amount of blood drawn. Conclusion: Iatrogenic blood loss for diagnostic purposes contributes significantly to the development and exacerbation of hospital-acquired anemia. Therefore, a comprehensive PBM in intensive care is urgently needed to reduce avoidable blood loss, including blood-sparing techniques, regular advanced training, and small-volume blood collection tubes.

Preanalytical robustness of blood collection tubes with RNA stabilizers

2019 ◽  
Vol 57 (10) ◽  
pp. 1522-1529 ◽  
Author(s):  
Chiara Stellino ◽  
Gaël Hamot ◽  
Camille Bellora ◽  
Johanna Trouet ◽  
Fay Betsou
Keyword(s):  
Storage Temperature ◽  
Regulation Of Gene Expression ◽  
Analysis Data ◽  
Blood Collection ◽  
Becton Dickinson ◽  
Rna Integrity ◽  
Qrt Pcr ◽  
Rna Integrity Number ◽  
Significant Difference ◽  
Blood Collection Tubes

Abstract Background Efficient blood stabilization is essential to obtaining reliable and comparable RNA analysis data in preclinical operations. PAXgene (Qiagen, Becton Dickinson) and Tempus (Applied Biosystems, Life Technologies) blood collection tubes with RNA stabilizers both avoid preanalytical degradation of mRNA by endogenous nucleases and modifications in specific mRNA concentrations by unintentional up- or down-regulation of gene expression. Methods Sixteen different preanalytical conditions were tested in PAXgene and Tempus blood samples from seven donors: different mixing after collection, different fill volumes and different 24-h transport temperature conditions after collection. RNA was extracted by column-based methods. The quality of the extracted RNA was assessed by spectrophotometric quantification, A260/A280 purity ratio, RNA Integrity Number (Agilent Bioanalyzer), miRNA quantative real time polymerase chain reaction (qRT-PCR) on two target miRNAs (RNU-24 and miR-16), mRNA quality index by qRT-PCR on the 3′ and 5′ region of the GAPDH gene, and the PBMC preanalytical score, based on the relative expression levels of the IL8 and EDEM3 coding genes. Results When PAXgene RNA and Tempus blood collection tubes were used following the manufacturers’ instructions, there was no statistically or technically significant difference in the output RNA quality attributes. However, the integrity of the RNA extracted from Tempus collection tubes was more sensitive to fill volumes and effective inversion, than to storage temperature, while the integrity of RNA extracted from PAXgene collection tubes was more sensitive to effective inversion and storage temperature than to fill volumes. Conclusions Blood collection tubes with different RNA stabilizers present different robustness to common preanalytical variations.

Sign in / Sign up

Export Citation Format

Share Document